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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-8302540-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=8302540&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 8302540,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_016579.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.772C>T",
          "hgvs_p": "p.Arg258Cys",
          "transcript": "NM_016579.4",
          "protein_id": "NP_057663.1",
          "transcript_support_level": null,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 282,
          "cds_start": 772,
          "cds_end": null,
          "cds_length": 849,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000301458.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016579.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.772C>T",
          "hgvs_p": "p.Arg258Cys",
          "transcript": "ENST00000301458.10",
          "protein_id": "ENSP00000301458.4",
          "transcript_support_level": 1,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 282,
          "cds_start": 772,
          "cds_end": null,
          "cds_length": 849,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_016579.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000301458.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "n.*1060C>T",
          "hgvs_p": null,
          "transcript": "ENST00000596002.5",
          "protein_id": "ENSP00000471773.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000596002.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "n.*1060C>T",
          "hgvs_p": null,
          "transcript": "ENST00000596002.5",
          "protein_id": "ENSP00000471773.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000596002.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.1084C>T",
          "hgvs_p": "p.Arg362Cys",
          "transcript": "ENST00000963189.1",
          "protein_id": "ENSP00000633248.1",
          "transcript_support_level": null,
          "aa_start": 362,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": 1084,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963189.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.817C>T",
          "hgvs_p": "p.Arg273Cys",
          "transcript": "ENST00000932616.1",
          "protein_id": "ENSP00000602675.1",
          "transcript_support_level": null,
          "aa_start": 273,
          "aa_end": null,
          "aa_length": 297,
          "cds_start": 817,
          "cds_end": null,
          "cds_length": 894,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000932616.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.796C>T",
          "hgvs_p": "p.Arg266Cys",
          "transcript": "ENST00000874639.1",
          "protein_id": "ENSP00000544698.1",
          "transcript_support_level": null,
          "aa_start": 266,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 796,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874639.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.679C>T",
          "hgvs_p": "p.Arg227Cys",
          "transcript": "ENST00000932619.1",
          "protein_id": "ENSP00000602678.1",
          "transcript_support_level": null,
          "aa_start": 227,
          "aa_end": null,
          "aa_length": 251,
          "cds_start": 679,
          "cds_end": null,
          "cds_length": 756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000932619.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.646C>T",
          "hgvs_p": "p.Arg216Cys",
          "transcript": "NM_001165895.2",
          "protein_id": "NP_001159367.1",
          "transcript_support_level": null,
          "aa_start": 216,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": 646,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001165895.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.646C>T",
          "hgvs_p": "p.Arg216Cys",
          "transcript": "ENST00000537716.6",
          "protein_id": "ENSP00000437697.1",
          "transcript_support_level": 2,
          "aa_start": 216,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": 646,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000537716.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.640C>T",
          "hgvs_p": "p.Arg214Cys",
          "transcript": "ENST00000874640.1",
          "protein_id": "ENSP00000544699.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 238,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 717,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874640.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.637C>T",
          "hgvs_p": "p.Arg213Cys",
          "transcript": "ENST00000874638.1",
          "protein_id": "ENSP00000544697.1",
          "transcript_support_level": null,
          "aa_start": 213,
          "aa_end": null,
          "aa_length": 237,
          "cds_start": 637,
          "cds_end": null,
          "cds_length": 714,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874638.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.538C>T",
          "hgvs_p": "p.Arg180Cys",
          "transcript": "ENST00000932617.1",
          "protein_id": "ENSP00000602676.1",
          "transcript_support_level": null,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000932617.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.514C>T",
          "hgvs_p": "p.Arg172Cys",
          "transcript": "ENST00000932621.1",
          "protein_id": "ENSP00000602680.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 196,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 591,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000932621.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.511C>T",
          "hgvs_p": "p.Arg171Cys",
          "transcript": "ENST00000932620.1",
          "protein_id": "ENSP00000602679.1",
          "transcript_support_level": null,
          "aa_start": 171,
          "aa_end": null,
          "aa_length": 195,
          "cds_start": 511,
          "cds_end": null,
          "cds_length": 588,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000932620.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.412C>T",
          "hgvs_p": "p.Arg138Cys",
          "transcript": "ENST00000874637.1",
          "protein_id": "ENSP00000544696.1",
          "transcript_support_level": null,
          "aa_start": 138,
          "aa_end": null,
          "aa_length": 162,
          "cds_start": 412,
          "cds_end": null,
          "cds_length": 489,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874637.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "c.208C>T",
          "hgvs_p": "p.Arg70Cys",
          "transcript": "ENST00000932618.1",
          "protein_id": "ENSP00000602677.1",
          "transcript_support_level": null,
          "aa_start": 70,
          "aa_end": null,
          "aa_length": 94,
          "cds_start": 208,
          "cds_end": null,
          "cds_length": 285,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000932618.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "n.*372C>T",
          "hgvs_p": null,
          "transcript": "ENST00000599573.1",
          "protein_id": "ENSP00000471551.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000599573.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD320",
          "gene_hgnc_id": 16692,
          "hgvs_c": "n.*372C>T",
          "hgvs_p": null,
          "transcript": "ENST00000599573.1",
          "protein_id": "ENSP00000471551.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000599573.1"
        }
      ],
      "gene_symbol": "CD320",
      "gene_hgnc_id": 16692,
      "dbsnp": "rs146190802",
      "frequency_reference_population": 0.009123897,
      "hom_count_reference_population": 1302,
      "allele_count_reference_population": 14724,
      "gnomad_exomes_af": 0.00948816,
      "gnomad_genomes_af": 0.00562786,
      "gnomad_exomes_ac": 13867,
      "gnomad_genomes_ac": 857,
      "gnomad_exomes_homalt": 1206,
      "gnomad_genomes_homalt": 96,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0018918812274932861,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.171,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2688,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.22,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.29,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_016579.4",
          "gene_symbol": "CD320",
          "hgnc_id": 16692,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "Unknown,AR",
          "hgvs_c": "c.772C>T",
          "hgvs_p": "p.Arg258Cys"
        }
      ],
      "clinvar_disease": "Methylmalonic acidemia due to transcobalamin receptor defect,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:3",
      "phenotype_combined": "not specified|Methylmalonic acidemia due to transcobalamin receptor defect|not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}