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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-112553197-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=112553197&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 112553197,
      "ref": "A",
      "alt": "C",
      "effect": "intron_variant",
      "transcript": "ENST00000263331.10",
      "consequences": [
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.1158+381A>C",
          "hgvs_p": null,
          "transcript": "NM_019014.6",
          "protein_id": "NP_061887.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1135,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7527,
          "mane_select": "ENST00000263331.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.1158+381A>C",
          "hgvs_p": null,
          "transcript": "ENST00000263331.10",
          "protein_id": "ENSP00000263331.5",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1135,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3408,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7527,
          "mane_select": "NM_019014.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.1158+381A>C",
          "hgvs_p": null,
          "transcript": "ENST00000409894.7",
          "protein_id": "ENSP00000387143.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 952,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2859,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4457,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "n.*1028+381A>C",
          "hgvs_p": null,
          "transcript": "ENST00000333990.10",
          "protein_id": "ENSP00000334589.6",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4376,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "n.177+10526A>C",
          "hgvs_p": null,
          "transcript": "ENST00000448770.5",
          "protein_id": "ENSP00000390274.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2494,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.1158+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001371969.1",
          "protein_id": "NP_001358898.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1181,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3546,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7665,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.1272+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001282772.2",
          "protein_id": "NP_001269701.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1173,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3522,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7727,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.1272+381A>C",
          "hgvs_p": null,
          "transcript": "ENST00000541869.5",
          "protein_id": "ENSP00000444136.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1173,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3522,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5059,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.990+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001137604.3",
          "protein_id": "NP_001131076.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1079,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3240,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7359,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.990+381A>C",
          "hgvs_p": null,
          "transcript": "ENST00000417433.6",
          "protein_id": "ENSP00000405358.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 1079,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 3240,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3836,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.741+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001282777.2",
          "protein_id": "NP_001269706.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 996,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2991,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7619,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.741+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001282779.2",
          "protein_id": "NP_001269708.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 996,
          "cds_start": -4,
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          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.741+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001371970.1",
          "protein_id": "NP_001358899.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 996,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2991,
          "cdna_start": null,
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          "cdna_length": 7785,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.1158+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001282774.2",
          "protein_id": "NP_001269703.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 952,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2859,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6978,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.525+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001282776.2",
          "protein_id": "NP_001269705.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 924,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2775,
          "cdna_start": null,
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          "cdna_length": 7079,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.525+381A>C",
          "hgvs_p": null,
          "transcript": "NM_001371971.1",
          "protein_id": "NP_001358900.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 924,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2775,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 7394,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "c.525+381A>C",
          "hgvs_p": null,
          "transcript": "ENST00000537335.5",
          "protein_id": "ENSP00000437914.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 924,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2775,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4961,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POLR1B",
          "gene_hgnc_id": 20454,
          "hgvs_c": "n.-188A>C",
          "hgvs_p": null,
          "transcript": "ENST00000498054.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 636,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "POLR1B",
      "gene_hgnc_id": 20454,
      "dbsnp": "rs9308672",
      "frequency_reference_population": 0.0000065747086,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": 0.00000657471,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.9200000166893005,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.92,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.416,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000263331.10",
          "gene_symbol": "POLR1B",
          "hgnc_id": 20454,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1158+381A>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}