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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-127639361-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=127639361&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 127639361,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_017980.5",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.946C>G",
          "hgvs_p": "p.Leu316Val",
          "transcript": "NM_001161403.3",
          "protein_id": "NP_001154875.1",
          "transcript_support_level": null,
          "aa_start": 316,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 946,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000355119.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001161403.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.946C>G",
          "hgvs_p": "p.Leu316Val",
          "transcript": "ENST00000355119.9",
          "protein_id": "ENSP00000347240.4",
          "transcript_support_level": 1,
          "aa_start": 316,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 946,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001161403.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355119.9"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1018C>G",
          "hgvs_p": "p.Leu340Val",
          "transcript": "ENST00000324938.9",
          "protein_id": "ENSP00000326888.5",
          "transcript_support_level": 1,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 1018,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000324938.9"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.931C>G",
          "hgvs_p": "p.Leu311Val",
          "transcript": "ENST00000409455.5",
          "protein_id": "ENSP00000386383.1",
          "transcript_support_level": 1,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 931,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409455.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.931C>G",
          "hgvs_p": "p.Leu311Val",
          "transcript": "ENST00000410011.5",
          "protein_id": "ENSP00000387002.1",
          "transcript_support_level": 1,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 931,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000410011.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.490C>G",
          "hgvs_p": "p.Leu164Val",
          "transcript": "ENST00000409754.5",
          "protein_id": "ENSP00000386345.1",
          "transcript_support_level": 1,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 189,
          "cds_start": 490,
          "cds_end": null,
          "cds_length": 570,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409754.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1465C>G",
          "hgvs_p": "p.Leu489Val",
          "transcript": "ENST00000855737.1",
          "protein_id": "ENSP00000525796.1",
          "transcript_support_level": null,
          "aa_start": 489,
          "aa_end": null,
          "aa_length": 514,
          "cds_start": 1465,
          "cds_end": null,
          "cds_length": 1545,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855737.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1354C>G",
          "hgvs_p": "p.Leu452Val",
          "transcript": "ENST00000855740.1",
          "protein_id": "ENSP00000525799.1",
          "transcript_support_level": null,
          "aa_start": 452,
          "aa_end": null,
          "aa_length": 477,
          "cds_start": 1354,
          "cds_end": null,
          "cds_length": 1434,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855740.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1342C>G",
          "hgvs_p": "p.Leu448Val",
          "transcript": "ENST00000855742.1",
          "protein_id": "ENSP00000525801.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 1342,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855742.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1183C>G",
          "hgvs_p": "p.Leu395Val",
          "transcript": "ENST00000957032.1",
          "protein_id": "ENSP00000627091.1",
          "transcript_support_level": null,
          "aa_start": 395,
          "aa_end": null,
          "aa_length": 420,
          "cds_start": 1183,
          "cds_end": null,
          "cds_length": 1263,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957032.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1120C>G",
          "hgvs_p": "p.Leu374Val",
          "transcript": "ENST00000957031.1",
          "protein_id": "ENSP00000627090.1",
          "transcript_support_level": null,
          "aa_start": 374,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 1120,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957031.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1024C>G",
          "hgvs_p": "p.Leu342Val",
          "transcript": "ENST00000855738.1",
          "protein_id": "ENSP00000525797.1",
          "transcript_support_level": null,
          "aa_start": 342,
          "aa_end": null,
          "aa_length": 367,
          "cds_start": 1024,
          "cds_end": null,
          "cds_length": 1104,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855738.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1018C>G",
          "hgvs_p": "p.Leu340Val",
          "transcript": "NM_017980.5",
          "protein_id": "NP_060450.2",
          "transcript_support_level": null,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 1018,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017980.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1012C>G",
          "hgvs_p": "p.Leu338Val",
          "transcript": "NM_001136037.4",
          "protein_id": "NP_001129509.2",
          "transcript_support_level": null,
          "aa_start": 338,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 1012,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001136037.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1012C>G",
          "hgvs_p": "p.Leu338Val",
          "transcript": "ENST00000545738.6",
          "protein_id": "ENSP00000443794.2",
          "transcript_support_level": 5,
          "aa_start": 338,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 1012,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000545738.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.1006C>G",
          "hgvs_p": "p.Leu336Val",
          "transcript": "ENST00000855739.1",
          "protein_id": "ENSP00000525798.1",
          "transcript_support_level": null,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 1006,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855739.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.958C>G",
          "hgvs_p": "p.Leu320Val",
          "transcript": "ENST00000855741.1",
          "protein_id": "ENSP00000525800.1",
          "transcript_support_level": null,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 958,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855741.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.931C>G",
          "hgvs_p": "p.Leu311Val",
          "transcript": "NM_001161404.2",
          "protein_id": "NP_001154876.1",
          "transcript_support_level": null,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 931,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001161404.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.931C>G",
          "hgvs_p": "p.Leu311Val",
          "transcript": "ENST00000409808.6",
          "protein_id": "ENSP00000386637.2",
          "transcript_support_level": 5,
          "aa_start": 311,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 931,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409808.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LIMS2",
          "gene_hgnc_id": 16084,
          "hgvs_c": "c.829C>G",
          "hgvs_p": "p.Leu277Val",
          "transcript": "ENST00000855743.1",
          "protein_id": "ENSP00000525802.1",
          "transcript_support_level": null,
          "aa_start": 277,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 829,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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        {
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          "exon_count": 2,
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          "hgvs_c": "n.202C>G",
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          "biotype": "pseudogene",
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        {
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          "protein_coding": false,
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          "consequences": [
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          "exon_count": 6,
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          "gene_symbol": "LIMS2",
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          "hgvs_c": "n.*156C>G",
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          "transcript": "ENST00000413578.5",
          "protein_id": "ENSP00000388611.1",
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000413578.5"
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      ],
      "gene_symbol": "LIMS2",
      "gene_hgnc_id": 16084,
      "dbsnp": "rs1454700502",
      "frequency_reference_population": 0.0000012392665,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 6.84126e-7,
      "gnomad_genomes_af": 0.00000657298,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.26992267370224,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.146,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.5898,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.25,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.875,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_017980.5",
          "gene_symbol": "LIMS2",
          "hgnc_id": 16084,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.1018C>G",
          "hgvs_p": "p.Leu340Val"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}