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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-130152705-C-CCGGTAACTGCCCAGGAAG (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=130152705&ref=C&alt=CCGGTAACTGCCCAGGAAG&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 130152705,
      "ref": "C",
      "alt": "CCGGTAACTGCCCAGGAAG",
      "effect": "disruptive_inframe_insertion",
      "transcript": "NM_017751.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2316_2333dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg778_Thr779insPheLeuGlySerTyrArg",
          "transcript": "NM_017951.5",
          "protein_id": "NP_060421.3",
          "transcript_support_level": null,
          "aa_start": 778,
          "aa_end": null,
          "aa_length": 827,
          "cds_start": 2333,
          "cds_end": null,
          "cds_length": 2484,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000680298.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017951.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2316_2333dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg778_Thr779insPheLeuGlySerTyrArg",
          "transcript": "ENST00000680298.1",
          "protein_id": "ENSP00000506463.1",
          "transcript_support_level": null,
          "aa_start": 778,
          "aa_end": null,
          "aa_length": 827,
          "cds_start": 2333,
          "cds_end": null,
          "cds_length": 2484,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017951.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680298.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2433_2450dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg817_Thr818insPheLeuGlySerTyrArg",
          "transcript": "ENST00000409031.5",
          "protein_id": "ENSP00000386531.1",
          "transcript_support_level": 1,
          "aa_start": 817,
          "aa_end": null,
          "aa_length": 866,
          "cds_start": 2450,
          "cds_end": null,
          "cds_length": 2601,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409031.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "n.*2020_*2037dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": null,
          "transcript": "ENST00000454468.5",
          "protein_id": "ENSP00000407591.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000454468.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "n.1256_1273dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": null,
          "transcript": "ENST00000491128.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000491128.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "n.*2020_*2037dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": null,
          "transcript": "ENST00000454468.5",
          "protein_id": "ENSP00000407591.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000454468.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2367_2384dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg795_Thr796insPheLeuGlySerTyrArg",
          "transcript": "ENST00000680679.1",
          "protein_id": "ENSP00000505716.1",
          "transcript_support_level": null,
          "aa_start": 795,
          "aa_end": null,
          "aa_length": 844,
          "cds_start": 2384,
          "cds_end": null,
          "cds_length": 2535,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680679.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2346_2363dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg788_Thr789insPheLeuGlySerTyrArg",
          "transcript": "NM_017751.4",
          "protein_id": "NP_060221.2",
          "transcript_support_level": null,
          "aa_start": 788,
          "aa_end": null,
          "aa_length": 837,
          "cds_start": 2363,
          "cds_end": null,
          "cds_length": 2514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017751.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2346_2363dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg788_Thr789insPheLeuGlySerTyrArg",
          "transcript": "ENST00000351288.10",
          "protein_id": "ENSP00000259217.8",
          "transcript_support_level": 2,
          "aa_start": 788,
          "aa_end": null,
          "aa_length": 837,
          "cds_start": 2363,
          "cds_end": null,
          "cds_length": 2514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000351288.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2316_2333dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg778_Thr779insPheLeuGlySerTyrArg",
          "transcript": "ENST00000883567.1",
          "protein_id": "ENSP00000553626.1",
          "transcript_support_level": null,
          "aa_start": 778,
          "aa_end": null,
          "aa_length": 827,
          "cds_start": 2333,
          "cds_end": null,
          "cds_length": 2484,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883567.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2316_2333dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg778_Thr779insPheLeuGlySerTyrArg",
          "transcript": "ENST00000951021.1",
          "protein_id": "ENSP00000621080.1",
          "transcript_support_level": null,
          "aa_start": 778,
          "aa_end": null,
          "aa_length": 827,
          "cds_start": 2333,
          "cds_end": null,
          "cds_length": 2484,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951021.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2298_2315dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg772_Thr773insPheLeuGlySerTyrArg",
          "transcript": "ENST00000883569.1",
          "protein_id": "ENSP00000553628.1",
          "transcript_support_level": null,
          "aa_start": 772,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 2315,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883569.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2187_2204dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg735_Thr736insPheLeuGlySerTyrArg",
          "transcript": "ENST00000883570.1",
          "protein_id": "ENSP00000553629.1",
          "transcript_support_level": null,
          "aa_start": 735,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 2204,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883570.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2127_2144dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg715_Thr716insPheLeuGlySerTyrArg",
          "transcript": "NM_001171083.2",
          "protein_id": "NP_001164554.1",
          "transcript_support_level": null,
          "aa_start": 715,
          "aa_end": null,
          "aa_length": 764,
          "cds_start": 2144,
          "cds_end": null,
          "cds_length": 2295,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001171083.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2127_2144dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg715_Thr716insPheLeuGlySerTyrArg",
          "transcript": "ENST00000431183.6",
          "protein_id": "ENSP00000405187.2",
          "transcript_support_level": 2,
          "aa_start": 715,
          "aa_end": null,
          "aa_length": 764,
          "cds_start": 2144,
          "cds_end": null,
          "cds_length": 2295,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000431183.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2100_2117dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg706_Thr707insPheLeuGlySerTyrArg",
          "transcript": "ENST00000883568.1",
          "protein_id": "ENSP00000553627.1",
          "transcript_support_level": null,
          "aa_start": 706,
          "aa_end": null,
          "aa_length": 755,
          "cds_start": 2117,
          "cds_end": null,
          "cds_length": 2268,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883568.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.2055_2072dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg691_Thr692insPheLeuGlySerTyrArg",
          "transcript": "ENST00000439886.5",
          "protein_id": "ENSP00000401648.1",
          "transcript_support_level": 2,
          "aa_start": 691,
          "aa_end": null,
          "aa_length": 740,
          "cds_start": 2072,
          "cds_end": null,
          "cds_length": 2223,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000439886.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.1119_1136dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg379_Thr380insPheLeuGlySerTyrArg",
          "transcript": "ENST00000680401.1",
          "protein_id": "ENSP00000506367.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 428,
          "cds_start": 1136,
          "cds_end": null,
          "cds_length": 1287,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680401.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "RFLGSYR",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "disruptive_inframe_insertion"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
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      "gene_hgnc_id": 32949,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": null,
      "splice_prediction_selected": null,
      "splice_source_selected": null,
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 4.139,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": null,
      "spliceai_max_prediction": null,
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM4",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_017751.4",
          "gene_symbol": "SMPD4",
          "hgnc_id": 32949,
          "effects": [
            "disruptive_inframe_insertion"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2346_2363dupCTTCCTGGGCAGTTACCG",
          "hgvs_p": "p.Arg788_Thr789insPheLeuGlySerTyrArg"
        }
      ],
      "clinvar_disease": " and structural brain anomalies, arthrogryposis,Neurodevelopmental disorder with microcephaly,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "phenotype_combined": "Neurodevelopmental disorder with microcephaly, arthrogryposis, and structural brain anomalies|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}