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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-130182354-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=130182354&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 130182354,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001330282.2",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "NM_025029.5",
          "protein_id": "NP_079305.2",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": 93,
          "cdna_end": null,
          "cdna_length": 599,
          "mane_select": "ENST00000281871.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_025029.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "ENST00000281871.11",
          "protein_id": "ENSP00000281871.7",
          "transcript_support_level": 1,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": 93,
          "cdna_end": null,
          "cdna_length": 599,
          "mane_select": "NM_025029.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000281871.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.-753C>A",
          "hgvs_p": null,
          "transcript": "ENST00000409031.5",
          "protein_id": "ENSP00000386531.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 866,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2601,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4896,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409031.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "NM_001330282.2",
          "protein_id": "NP_001317211.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 657,
          "cdna_start": 93,
          "cdna_end": null,
          "cdna_length": 779,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330282.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "ENST00000409255.1",
          "protein_id": "ENSP00000386419.1",
          "transcript_support_level": 5,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 657,
          "cdna_start": 98,
          "cdna_end": null,
          "cdna_length": 769,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409255.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "ENST00000948926.1",
          "protein_id": "ENSP00000618985.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 201,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 606,
          "cdna_start": 82,
          "cdna_end": null,
          "cdna_length": 702,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948926.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "ENST00000916359.1",
          "protein_id": "ENSP00000586418.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 175,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 528,
          "cdna_start": 88,
          "cdna_end": null,
          "cdna_length": 631,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916359.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "ENST00000651060.1",
          "protein_id": "ENSP00000498754.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 131,
          "cdna_end": null,
          "cdna_length": 603,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000651060.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "ENST00000916358.1",
          "protein_id": "ENSP00000586417.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 141,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 426,
          "cdna_start": 93,
          "cdna_end": null,
          "cdna_length": 545,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916358.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "NM_001330284.2",
          "protein_id": "NP_001317213.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 130,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 393,
          "cdna_start": 93,
          "cdna_end": null,
          "cdna_length": 450,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330284.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.42G>T",
          "hgvs_p": "p.Lys14Asn",
          "transcript": "ENST00000455239.1",
          "protein_id": "ENSP00000404629.1",
          "transcript_support_level": 2,
          "aa_start": 14,
          "aa_end": null,
          "aa_length": 120,
          "cds_start": 42,
          "cds_end": null,
          "cds_length": 363,
          "cdna_start": 42,
          "cdna_end": null,
          "cdna_length": 401,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000455239.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "XM_047445914.1",
          "protein_id": "XP_047301870.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 153,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 462,
          "cdna_start": 93,
          "cdna_end": null,
          "cdna_length": 1080,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047445914.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn",
          "transcript": "XM_047445916.1",
          "protein_id": "XP_047301872.1",
          "transcript_support_level": null,
          "aa_start": 24,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": 72,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": 93,
          "cdna_end": null,
          "cdna_length": 1558,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_047445916.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.-136C>A",
          "hgvs_p": null,
          "transcript": "ENST00000883567.1",
          "protein_id": "ENSP00000553626.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_start": null,
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          "cdna_start": null,
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        },
        {
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          ],
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          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.60-273G>T",
          "hgvs_p": null,
          "transcript": "ENST00000425361.5",
          "protein_id": "ENSP00000398749.1",
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          "aa_start": null,
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          "cds_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000425361.5"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.60-273G>T",
          "hgvs_p": null,
          "transcript": "ENST00000457492.5",
          "protein_id": "ENSP00000387805.1",
          "transcript_support_level": 5,
          "aa_start": null,
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          "cds_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": null,
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          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "c.37-273G>T",
          "hgvs_p": null,
          "transcript": "XM_047445915.1",
          "protein_id": "XP_047301871.1",
          "transcript_support_level": null,
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        },
        {
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          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "n.-201G>T",
          "hgvs_p": null,
          "transcript": "ENST00000480182.1",
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          "transcript_support_level": 1,
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          "mane_select": null,
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          "biotype": "retained_intron",
          "feature": "ENST00000480182.1"
        },
        {
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          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": null,
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          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMPD4",
          "gene_hgnc_id": 32949,
          "hgvs_c": "c.-451C>A",
          "hgvs_p": null,
          "transcript": "ENST00000951021.1",
          "protein_id": "ENSP00000621080.1",
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          "cds_start": null,
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          "cdna_length": 3834,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000951021.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MZT2B",
          "gene_hgnc_id": 25886,
          "hgvs_c": "n.-61G>T",
          "hgvs_p": null,
          "transcript": "ENST00000485869.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 583,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000485869.1"
        }
      ],
      "gene_symbol": "MZT2B",
      "gene_hgnc_id": 25886,
      "dbsnp": "rs1296290910",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.4034600853919983,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.174,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.8753,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.41,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.857,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001330282.2",
          "gene_symbol": "MZT2B",
          "hgnc_id": 25886,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.72G>T",
          "hgvs_p": "p.Lys24Asn"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000409031.5",
          "gene_symbol": "SMPD4",
          "hgnc_id": 32949,
          "effects": [
            "5_prime_UTR_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-753C>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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