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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-130931121-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=130931121&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "ARHGEF4",
          "hgnc_id": 684,
          "hgvs_c": "c.3722C>G",
          "hgvs_p": "p.Pro1241Arg",
          "inheritance_mode": "AR",
          "pathogenic_score": 2,
          "score": 0,
          "transcript": "NM_001367493.1",
          "verdict": "Uncertain_significance"
        }
      ],
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_score": 0,
      "allele_count_reference_population": 2,
      "alphamissense_prediction": null,
      "alphamissense_score": 0.0739,
      "alt": "G",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Benign",
      "bayesdelnoaf_score": -0.33,
      "chr": "2",
      "clinvar_classification": "",
      "clinvar_disease": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "computational_prediction_selected": "Benign",
      "computational_score_selected": 0.14698266983032227,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 1876,
          "aa_ref": "P",
          "aa_start": 1241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 6735,
          "cdna_start": 3762,
          "cds_end": null,
          "cds_length": 5631,
          "cds_start": 3722,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "NM_001367493.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.3722C>G",
          "hgvs_p": "p.Pro1241Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000409359.7",
          "protein_coding": true,
          "protein_id": "NP_001354422.1",
          "strand": true,
          "transcript": "NM_001367493.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 1876,
          "aa_ref": "P",
          "aa_start": 1241,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 6735,
          "cdna_start": 3762,
          "cds_end": null,
          "cds_length": 5631,
          "cds_start": 3722,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000409359.7",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.3722C>G",
          "hgvs_p": "p.Pro1241Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_001367493.1",
          "protein_coding": true,
          "protein_id": "ENSP00000386794.3",
          "strand": true,
          "transcript": "ENST00000409359.7",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 694,
          "aa_ref": "P",
          "aa_start": 79,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3800,
          "cdna_start": 238,
          "cds_end": null,
          "cds_length": 2085,
          "cds_start": 236,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000392953.8",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.236C>G",
          "hgvs_p": "p.Pro79Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000376680.5",
          "strand": true,
          "transcript": "ENST00000392953.8",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 1865,
          "aa_ref": "P",
          "aa_start": 1241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 6699,
          "cdna_start": 3761,
          "cds_end": null,
          "cds_length": 5598,
          "cds_start": 3722,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000918336.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.3722C>G",
          "hgvs_p": "p.Pro1241Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000588395.1",
          "strand": true,
          "transcript": "ENST00000918336.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 1827,
          "aa_ref": "P",
          "aa_start": 1241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 6587,
          "cdna_start": 3761,
          "cds_end": null,
          "cds_length": 5484,
          "cds_start": 3722,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000918335.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.3722C>G",
          "hgvs_p": "p.Pro1241Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000588394.1",
          "strand": true,
          "transcript": "ENST00000918335.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 1576,
          "aa_ref": "P",
          "aa_start": 1241,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 5813,
          "cdna_start": 3740,
          "cds_end": null,
          "cds_length": 4731,
          "cds_start": 3722,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000918338.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.3722C>G",
          "hgvs_p": "p.Pro1241Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000588397.1",
          "strand": true,
          "transcript": "ENST00000918338.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 705,
          "aa_ref": "P",
          "aa_start": 70,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3222,
          "cdna_start": 249,
          "cds_end": null,
          "cds_length": 2118,
          "cds_start": 209,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 13,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "NM_001375900.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.209C>G",
          "hgvs_p": "p.Pro70Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001362829.1",
          "strand": true,
          "transcript": "NM_001375900.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 690,
          "aa_ref": "P",
          "aa_start": 55,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 6400,
          "cdna_start": 3427,
          "cds_end": null,
          "cds_length": 2073,
          "cds_start": 164,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 15,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "NM_015320.4",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.164C>G",
          "hgvs_p": "p.Pro55Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_056135.2",
          "strand": true,
          "transcript": "NM_015320.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 690,
          "aa_ref": "P",
          "aa_start": 55,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3666,
          "cdna_start": 683,
          "cds_end": null,
          "cds_length": 2073,
          "cds_start": 164,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000326016.10",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.164C>G",
          "hgvs_p": "p.Pro55Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000316845.5",
          "strand": true,
          "transcript": "ENST00000326016.10",
          "transcript_support_level": 2
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 656,
          "aa_ref": "P",
          "aa_start": 70,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3065,
          "cdna_start": 239,
          "cds_end": null,
          "cds_length": 1971,
          "cds_start": 209,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 13,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000918337.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.209C>G",
          "hgvs_p": "p.Pro70Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000588396.1",
          "strand": true,
          "transcript": "ENST00000918337.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 653,
          "aa_ref": "P",
          "aa_start": 18,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3247,
          "cdna_start": 274,
          "cds_end": null,
          "cds_length": 1962,
          "cds_start": 53,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 16,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "NM_001375901.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.53C>G",
          "hgvs_p": "p.Pro18Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001362830.1",
          "strand": true,
          "transcript": "NM_001375901.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 630,
          "aa_ref": "P",
          "aa_start": 55,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3486,
          "cdna_start": 683,
          "cds_end": null,
          "cds_length": 1893,
          "cds_start": 164,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 13,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000611048.4",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.164C>G",
          "hgvs_p": "p.Pro55Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000483548.1",
          "strand": true,
          "transcript": "ENST00000611048.4",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "R",
          "aa_end": null,
          "aa_length": 372,
          "aa_ref": "P",
          "aa_start": 235,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1638,
          "cdna_start": 704,
          "cds_end": null,
          "cds_length": 1119,
          "cds_start": 704,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 4,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000438985.5",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.704C>G",
          "hgvs_p": "p.Pro235Arg",
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          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
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          "transcript_support_level": 2
        },
        {
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          "aa_end": null,
          "aa_length": 192,
          "aa_ref": "P",
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          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2172,
          "cdna_start": 683,
          "cds_end": null,
          "cds_length": 579,
          "cds_start": 164,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 5,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000428230.6",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "c.164C>G",
          "hgvs_p": "p.Pro55Arg",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000398455.2",
          "strand": true,
          "transcript": "ENST00000428230.6",
          "transcript_support_level": 5
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 4788,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000526381.2",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "n.3930C>G",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000526381.2",
          "transcript_support_level": 2
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "nonsense_mediated_decay",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3656,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 14,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000636987.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "n.*255C>G",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": "ENSP00000490717.1",
          "strand": true,
          "transcript": "ENST00000636987.1",
          "transcript_support_level": 2
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "nonsense_mediated_decay",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3656,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_count": 14,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "ENST00000636987.1",
          "gene_hgnc_id": 684,
          "gene_symbol": "ARHGEF4",
          "hgvs_c": "n.*255C>G",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": "ENSP00000490717.1",
          "strand": true,
          "transcript": "ENST00000636987.1",
          "transcript_support_level": 2
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs1383049756",
      "effect": "missense_variant",
      "frequency_reference_population": 0.0000013681388,
      "gene_hgnc_id": 684,
      "gene_symbol": "ARHGEF4",
      "gnomad_exomes_ac": 2,
      "gnomad_exomes_af": 0.00000136814,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": null,
      "gnomad_genomes_af": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": null,
      "phenotype_combined": null,
      "phylop100way_prediction": "Benign",
      "phylop100way_score": 0.833,
      "pos": 130931121,
      "ref": "C",
      "revel_prediction": "Benign",
      "revel_score": 0.091,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0.019999999552965164,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "transcript": "NM_001367493.1"
    }
  ]
}
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