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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-135846356-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=135846356&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 135846356,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_005915.6",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.2090A>G",
          "hgvs_p": "p.Asn697Ser",
          "transcript": "NM_005915.6",
          "protein_id": "NP_005906.2",
          "transcript_support_level": null,
          "aa_start": 697,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 2090,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": 2168,
          "cdna_end": null,
          "cdna_length": 3753,
          "mane_select": "ENST00000264156.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005915.6"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.2090A>G",
          "hgvs_p": "p.Asn697Ser",
          "transcript": "ENST00000264156.3",
          "protein_id": "ENSP00000264156.2",
          "transcript_support_level": 1,
          "aa_start": 697,
          "aa_end": null,
          "aa_length": 821,
          "cds_start": 2090,
          "cds_end": null,
          "cds_length": 2466,
          "cdna_start": 2168,
          "cdna_end": null,
          "cdna_length": 3753,
          "mane_select": "NM_005915.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000264156.3"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.2090A>G",
          "hgvs_p": "p.Asn697Ser",
          "transcript": "ENST00000884967.1",
          "protein_id": "ENSP00000555026.1",
          "transcript_support_level": null,
          "aa_start": 697,
          "aa_end": null,
          "aa_length": 820,
          "cds_start": 2090,
          "cds_end": null,
          "cds_length": 2463,
          "cdna_start": 2158,
          "cdna_end": null,
          "cdna_length": 2923,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884967.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.2087A>G",
          "hgvs_p": "p.Asn696Ser",
          "transcript": "ENST00000939153.1",
          "protein_id": "ENSP00000609212.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 820,
          "cds_start": 2087,
          "cds_end": null,
          "cds_length": 2463,
          "cdna_start": 2196,
          "cdna_end": null,
          "cdna_length": 2965,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939153.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.2078A>G",
          "hgvs_p": "p.Asn693Ser",
          "transcript": "ENST00000943623.1",
          "protein_id": "ENSP00000613682.1",
          "transcript_support_level": null,
          "aa_start": 693,
          "aa_end": null,
          "aa_length": 817,
          "cds_start": 2078,
          "cds_end": null,
          "cds_length": 2454,
          "cdna_start": 2148,
          "cdna_end": null,
          "cdna_length": 2912,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943623.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.1979A>G",
          "hgvs_p": "p.Asn660Ser",
          "transcript": "ENST00000939152.1",
          "protein_id": "ENSP00000609211.1",
          "transcript_support_level": null,
          "aa_start": 660,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 1979,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": 2093,
          "cdna_end": null,
          "cdna_length": 2861,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939152.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.1943A>G",
          "hgvs_p": "p.Asn648Ser",
          "transcript": "ENST00000884968.1",
          "protein_id": "ENSP00000555027.1",
          "transcript_support_level": null,
          "aa_start": 648,
          "aa_end": null,
          "aa_length": 772,
          "cds_start": 1943,
          "cds_end": null,
          "cds_length": 2319,
          "cdna_start": 2011,
          "cdna_end": null,
          "cdna_length": 2776,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884968.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.1934A>G",
          "hgvs_p": "p.Asn645Ser",
          "transcript": "ENST00000939155.1",
          "protein_id": "ENSP00000609214.1",
          "transcript_support_level": null,
          "aa_start": 645,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": 1934,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": 2016,
          "cdna_end": null,
          "cdna_length": 2785,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939155.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.1928A>G",
          "hgvs_p": "p.Asn643Ser",
          "transcript": "ENST00000939156.1",
          "protein_id": "ENSP00000609215.1",
          "transcript_support_level": null,
          "aa_start": 643,
          "aa_end": null,
          "aa_length": 767,
          "cds_start": 1928,
          "cds_end": null,
          "cds_length": 2304,
          "cdna_start": 1975,
          "cdna_end": null,
          "cdna_length": 2739,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939156.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.1793A>G",
          "hgvs_p": "p.Asn598Ser",
          "transcript": "ENST00000943622.1",
          "protein_id": "ENSP00000613681.1",
          "transcript_support_level": null,
          "aa_start": 598,
          "aa_end": null,
          "aa_length": 722,
          "cds_start": 1793,
          "cds_end": null,
          "cds_length": 2169,
          "cdna_start": 1893,
          "cdna_end": null,
          "cdna_length": 2659,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943622.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": 14,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "c.2054-1672A>G",
          "hgvs_p": null,
          "transcript": "ENST00000939154.1",
          "protein_id": "ENSP00000609213.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 769,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2310,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2788,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939154.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCM6",
          "gene_hgnc_id": 6949,
          "hgvs_c": "n.516A>G",
          "hgvs_p": null,
          "transcript": "ENST00000492091.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 712,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000492091.1"
        }
      ],
      "gene_symbol": "MCM6",
      "gene_hgnc_id": 6949,
      "dbsnp": "rs560524153",
      "frequency_reference_population": 0.000018586716,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 30,
      "gnomad_exomes_af": 0.0000184695,
      "gnomad_genomes_af": 0.0000197125,
      "gnomad_exomes_ac": 27,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0749674141407013,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.105,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0686,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.71,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.642,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_005915.6",
          "gene_symbol": "MCM6",
          "hgnc_id": 6949,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2090A>G",
          "hgvs_p": "p.Asn697Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
For research and educational, non-commercial use only. Not for clinical or diagnostic use. GeneBe does not provide medical advice. Data use for AI modeling is prohibited: if used, the cost is $0.001 per byte of downloaded uncompressed data.