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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-144517341-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=144517341&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 144517341,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_014795.4",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "NM_014795.4",
          "protein_id": "NP_055610.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 3645,
          "cdna_start": 260,
          "cdna_end": null,
          "cdna_length": 9265,
          "mane_select": "ENST00000627532.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014795.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000627532.3",
          "protein_id": "ENSP00000487174.1",
          "transcript_support_level": 1,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 3645,
          "cdna_start": 260,
          "cdna_end": null,
          "cdna_length": 9265,
          "mane_select": "NM_014795.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000627532.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000558170.6",
          "protein_id": "ENSP00000454157.1",
          "transcript_support_level": 1,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 3645,
          "cdna_start": 211,
          "cdna_end": null,
          "cdna_length": 4012,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000558170.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000303660.8",
          "protein_id": "ENSP00000302501.4",
          "transcript_support_level": 1,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1213,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 3642,
          "cdna_start": 147,
          "cdna_end": null,
          "cdna_length": 3913,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000303660.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.97C>G",
          "hgvs_p": "p.Pro33Ala",
          "transcript": "ENST00000427902.5",
          "protein_id": "ENSP00000395496.2",
          "transcript_support_level": 1,
          "aa_start": 33,
          "aa_end": null,
          "aa_length": 730,
          "cds_start": 97,
          "cds_end": null,
          "cds_length": 2194,
          "cdna_start": 139,
          "cdna_end": null,
          "cdna_length": 2236,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000427902.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.94C>G",
          "hgvs_p": "p.Pro32Ala",
          "transcript": "ENST00000392861.6",
          "protein_id": "ENSP00000376601.3",
          "transcript_support_level": 1,
          "aa_start": 32,
          "aa_end": null,
          "aa_length": 466,
          "cds_start": 94,
          "cds_end": null,
          "cds_length": 1401,
          "cdna_start": 207,
          "cdna_end": null,
          "cdna_length": 1514,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000392861.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.97C>G",
          "hgvs_p": "p.Pro33Ala",
          "transcript": "ENST00000470879.5",
          "protein_id": "ENSP00000475329.2",
          "transcript_support_level": 1,
          "aa_start": 33,
          "aa_end": null,
          "aa_length": 87,
          "cds_start": 97,
          "cds_end": null,
          "cds_length": 264,
          "cdna_start": 176,
          "cdna_end": null,
          "cdna_length": 2648,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000470879.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "n.260C>G",
          "hgvs_p": null,
          "transcript": "ENST00000472146.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3837,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000472146.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "n.10C>G",
          "hgvs_p": null,
          "transcript": "ENST00000484313.3",
          "protein_id": "ENSP00000486341.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 845,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000484313.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000636471.1",
          "protein_id": "ENSP00000490317.1",
          "transcript_support_level": 5,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1239,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 3720,
          "cdna_start": 503,
          "cdna_end": null,
          "cdna_length": 9583,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636471.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000409487.7",
          "protein_id": "ENSP00000386854.2",
          "transcript_support_level": 5,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
          "cds_end": null,
          "cds_length": 3645,
          "cdna_start": 293,
          "cdna_end": null,
          "cdna_length": 5361,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409487.7"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000886176.1",
          "protein_id": "ENSP00000556235.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
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          "cds_length": 3645,
          "cdna_start": 122,
          "cdna_end": null,
          "cdna_length": 5192,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886176.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000886177.1",
          "protein_id": "ENSP00000556236.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
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          "cds_length": 3645,
          "cdna_start": 297,
          "cdna_end": null,
          "cdna_length": 5367,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886177.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000886179.1",
          "protein_id": "ENSP00000556238.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
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          "cds_length": 3645,
          "cdna_start": 260,
          "cdna_end": null,
          "cdna_length": 5330,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000886179.1"
        },
        {
          "aa_ref": "P",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 2,
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          "intron_rank": null,
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          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000965901.1",
          "protein_id": "ENSP00000635960.1",
          "transcript_support_level": null,
          "aa_start": 4,
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          "cds_start": 10,
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          "cds_length": 3645,
          "cdna_start": 133,
          "cdna_end": null,
          "cdna_length": 5196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965901.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000965902.1",
          "protein_id": "ENSP00000635961.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1214,
          "cds_start": 10,
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          "cds_length": 3645,
          "cdna_start": 135,
          "cdna_end": null,
          "cdna_length": 5203,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000965902.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000636026.2",
          "protein_id": "ENSP00000490776.1",
          "transcript_support_level": 5,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1202,
          "cds_start": 10,
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          "cds_length": 3609,
          "cdna_start": 539,
          "cdna_end": null,
          "cdna_length": 5172,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
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          "strand": false,
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          ],
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "NM_001171653.2",
          "protein_id": "NP_001165124.1",
          "transcript_support_level": null,
          "aa_start": 4,
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          "aa_length": 1190,
          "cds_start": 10,
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          "cds_length": 3573,
          "cdna_start": 260,
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          "cdna_length": 9193,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001171653.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
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          "protein_coding": true,
          "strand": false,
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          ],
          "exon_rank": 2,
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000539609.7",
          "protein_id": "ENSP00000443792.2",
          "transcript_support_level": 2,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1190,
          "cds_start": 10,
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          "cdna_start": 260,
          "cdna_end": null,
          "cdna_length": 4061,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000539609.7"
        },
        {
          "aa_ref": "P",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala",
          "transcript": "ENST00000886178.1",
          "protein_id": "ENSP00000556237.1",
          "transcript_support_level": null,
          "aa_start": 4,
          "aa_end": null,
          "aa_length": 1190,
          "cds_start": 10,
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          "cds_length": 3573,
          "cdna_start": 241,
          "cdna_end": null,
          "cdna_length": 5239,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886178.1"
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            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZEB2",
          "gene_hgnc_id": 14881,
          "hgvs_c": "n.260C>G",
          "hgvs_p": null,
          "transcript": "NR_033258.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2736,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_033258.2"
        }
      ],
      "gene_symbol": "ZEB2",
      "gene_hgnc_id": 14881,
      "dbsnp": "rs146394306",
      "frequency_reference_population": 0.00013819926,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 223,
      "gnomad_exomes_af": 0.000136857,
      "gnomad_genomes_af": 0.000151087,
      "gnomad_exomes_ac": 200,
      "gnomad_genomes_ac": 23,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.07305613160133362,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.175,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0846,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.23,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.364,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -14,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6_Very_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -14,
          "benign_score": 14,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_014795.4",
          "gene_symbol": "ZEB2",
          "hgnc_id": 14881,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.10C>G",
          "hgvs_p": "p.Pro4Ala"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases,Mowat-Wilson syndrome,ZEB2-related disorder,not provided",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:3 B:3",
      "phenotype_combined": "Mowat-Wilson syndrome|not provided|Inborn genetic diseases|ZEB2-related disorder",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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