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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-151485773-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=151485773&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 151485773,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001271208.2",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 182,
          "exon_rank_end": null,
          "exon_count": 182,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25565T>C",
          "hgvs_p": "p.Val8522Ala",
          "transcript": "NM_001164507.2",
          "protein_id": "NP_001157979.2",
          "transcript_support_level": null,
          "aa_start": 8522,
          "aa_end": null,
          "aa_length": 8525,
          "cds_start": 25565,
          "cds_end": null,
          "cds_length": 25578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": "ENST00000427231.7",
          "biotype": "protein_coding",
          "feature": "NM_001164507.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 182,
          "exon_rank_end": null,
          "exon_count": 182,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25565T>C",
          "hgvs_p": "p.Val8522Ala",
          "transcript": "NM_001164508.2",
          "protein_id": "NP_001157980.2",
          "transcript_support_level": null,
          "aa_start": 8522,
          "aa_end": null,
          "aa_length": 8525,
          "cds_start": 25565,
          "cds_end": null,
          "cds_length": 25578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000397345.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001164508.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 182,
          "exon_rank_end": null,
          "exon_count": 182,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25565T>C",
          "hgvs_p": "p.Val8522Ala",
          "transcript": "ENST00000397345.8",
          "protein_id": "ENSP00000380505.3",
          "transcript_support_level": 5,
          "aa_start": 8522,
          "aa_end": null,
          "aa_length": 8525,
          "cds_start": 25565,
          "cds_end": null,
          "cds_length": 25578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001164508.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000397345.8"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 182,
          "exon_rank_end": null,
          "exon_count": 182,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25565T>C",
          "hgvs_p": "p.Val8522Ala",
          "transcript": "ENST00000427231.7",
          "protein_id": "ENSP00000416578.2",
          "transcript_support_level": 5,
          "aa_start": 8522,
          "aa_end": null,
          "aa_length": 8525,
          "cds_start": 25565,
          "cds_end": null,
          "cds_length": 25578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": "NM_001164507.2",
          "biotype": "protein_coding",
          "feature": "ENST00000427231.7"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 183,
          "exon_rank_end": null,
          "exon_count": 183,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25670T>C",
          "hgvs_p": "p.Val8557Ala",
          "transcript": "NM_001271208.2",
          "protein_id": "NP_001258137.2",
          "transcript_support_level": null,
          "aa_start": 8557,
          "aa_end": null,
          "aa_length": 8560,
          "cds_start": 25670,
          "cds_end": null,
          "cds_length": 25683,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001271208.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 150,
          "exon_rank_end": null,
          "exon_count": 150,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.19997T>C",
          "hgvs_p": "p.Val6666Ala",
          "transcript": "NM_004543.5",
          "protein_id": "NP_004534.3",
          "transcript_support_level": null,
          "aa_start": 6666,
          "aa_end": null,
          "aa_length": 6669,
          "cds_start": 19997,
          "cds_end": null,
          "cds_length": 20010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004543.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 150,
          "exon_rank_end": null,
          "exon_count": 150,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.19997T>C",
          "hgvs_p": "p.Val6666Ala",
          "transcript": "ENST00000409198.5",
          "protein_id": "ENSP00000386259.1",
          "transcript_support_level": 5,
          "aa_start": 6666,
          "aa_end": null,
          "aa_length": 6669,
          "cds_start": 19997,
          "cds_end": null,
          "cds_length": 20010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409198.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 74,
          "exon_rank_end": null,
          "exon_count": 74,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.9011T>C",
          "hgvs_p": "p.Val3004Ala",
          "transcript": "ENST00000413693.5",
          "protein_id": "ENSP00000410961.1",
          "transcript_support_level": 5,
          "aa_start": 3004,
          "aa_end": null,
          "aa_length": 3007,
          "cds_start": 9011,
          "cds_end": null,
          "cds_length": 9024,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000413693.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 62,
          "exon_rank_end": null,
          "exon_count": 62,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.7268T>C",
          "hgvs_p": "p.Val2423Ala",
          "transcript": "ENST00000690043.1",
          "protein_id": "ENSP00000509961.1",
          "transcript_support_level": null,
          "aa_start": 2423,
          "aa_end": null,
          "aa_length": 2426,
          "cds_start": 7268,
          "cds_end": null,
          "cds_length": 7281,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000690043.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 36,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.3872T>C",
          "hgvs_p": "p.Val1291Ala",
          "transcript": "ENST00000434685.6",
          "protein_id": "ENSP00000389074.2",
          "transcript_support_level": 5,
          "aa_start": 1291,
          "aa_end": null,
          "aa_length": 1294,
          "cds_start": 3872,
          "cds_end": null,
          "cds_length": 3885,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000434685.6"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.2162T>C",
          "hgvs_p": "p.Val721Ala",
          "transcript": "ENST00000688578.1",
          "protein_id": "ENSP00000509628.1",
          "transcript_support_level": null,
          "aa_start": 721,
          "aa_end": null,
          "aa_length": 724,
          "cds_start": 2162,
          "cds_end": null,
          "cds_length": 2175,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000688578.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.1964T>C",
          "hgvs_p": "p.Val655Ala",
          "transcript": "ENST00000397337.6",
          "protein_id": "ENSP00000380498.2",
          "transcript_support_level": 5,
          "aa_start": 655,
          "aa_end": null,
          "aa_length": 658,
          "cds_start": 1964,
          "cds_end": null,
          "cds_length": 1977,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000397337.6"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 181,
          "exon_rank_end": null,
          "exon_count": 181,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25472T>C",
          "hgvs_p": "p.Val8491Ala",
          "transcript": "XM_005246590.3",
          "protein_id": "XP_005246647.1",
          "transcript_support_level": null,
          "aa_start": 8491,
          "aa_end": null,
          "aa_length": 8494,
          "cds_start": 25472,
          "cds_end": null,
          "cds_length": 25485,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005246590.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 181,
          "exon_rank_end": null,
          "exon_count": 181,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25472T>C",
          "hgvs_p": "p.Val8491Ala",
          "transcript": "XM_005246591.3",
          "protein_id": "XP_005246648.1",
          "transcript_support_level": null,
          "aa_start": 8491,
          "aa_end": null,
          "aa_length": 8494,
          "cds_start": 25472,
          "cds_end": null,
          "cds_length": 25485,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 181,
          "exon_rank_end": null,
          "exon_count": 181,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25472T>C",
          "hgvs_p": "p.Val8491Ala",
          "transcript": "XM_005246592.3",
          "protein_id": "XP_005246649.1",
          "transcript_support_level": null,
          "aa_start": 8491,
          "aa_end": null,
          "aa_length": 8494,
          "cds_start": 25472,
          "cds_end": null,
          "cds_length": 25485,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005246592.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 181,
          "exon_rank_end": null,
          "exon_count": 181,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25472T>C",
          "hgvs_p": "p.Val8491Ala",
          "transcript": "XM_005246593.3",
          "protein_id": "XP_005246650.1",
          "transcript_support_level": null,
          "aa_start": 8491,
          "aa_end": null,
          "aa_length": 8494,
          "cds_start": 25472,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_005246593.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 181,
          "exon_rank_end": null,
          "exon_count": 181,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25472T>C",
          "hgvs_p": "p.Val8491Ala",
          "transcript": "XM_005246594.3",
          "protein_id": "XP_005246651.1",
          "transcript_support_level": null,
          "aa_start": 8491,
          "aa_end": null,
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          "cds_start": 25472,
          "cds_end": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005246594.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 181,
          "exon_rank_end": null,
          "exon_count": 181,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25454T>C",
          "hgvs_p": "p.Val8485Ala",
          "transcript": "XM_017004177.2",
          "protein_id": "XP_016859666.1",
          "transcript_support_level": null,
          "aa_start": 8485,
          "aa_end": null,
          "aa_length": 8488,
          "cds_start": 25454,
          "cds_end": null,
          "cds_length": 25467,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017004177.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 180,
          "exon_rank_end": null,
          "exon_count": 180,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25379T>C",
          "hgvs_p": "p.Val8460Ala",
          "transcript": "XM_005246596.3",
          "protein_id": "XP_005246653.1",
          "transcript_support_level": null,
          "aa_start": 8460,
          "aa_end": null,
          "aa_length": 8463,
          "cds_start": 25379,
          "cds_end": null,
          "cds_length": 25392,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005246596.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 180,
          "exon_rank_end": null,
          "exon_count": 180,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NEB",
          "gene_hgnc_id": 7720,
          "hgvs_c": "c.25379T>C",
          "hgvs_p": "p.Val8460Ala",
          "transcript": "XM_005246597.3",
          "protein_id": "XP_005246654.1",
          "transcript_support_level": null,
          "aa_start": 8460,
          "aa_end": null,
          "aa_length": 8463,
          "cds_start": 25379,
          "cds_end": null,
          "cds_length": 25392,
          "cdna_start": null,
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      "dbsnp": "rs558019875",
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      "gnomad_exomes_homalt": 0,
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      "computational_score_selected": 0.8342077136039734,
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      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.564,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4386,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.24,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.325,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3,BP6",
      "acmg_by_gene": [
        {
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          "verdict": "Uncertain_significance",
          "transcript": "NM_001271208.2",
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        {
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          "verdict": "Uncertain_significance",
          "transcript": "ENST00000454583.6",
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          "inheritance_mode": "AR",
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      "clinvar_disease": "Inborn genetic diseases,Nemaline myopathy 2",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:1",
      "phenotype_combined": "Nemaline myopathy 2|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
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  ],
  "message": null
}