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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-164493166-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=164493166&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 164493166,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_004490.3",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1493A>C",
          "hgvs_p": "p.Glu498Ala",
          "transcript": "NM_004490.3",
          "protein_id": "NP_004481.2",
          "transcript_support_level": null,
          "aa_start": 498,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1493,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": 1666,
          "cdna_end": null,
          "cdna_length": 2415,
          "mane_select": "ENST00000263915.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004490.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1493A>C",
          "hgvs_p": "p.Glu498Ala",
          "transcript": "ENST00000263915.8",
          "protein_id": "ENSP00000263915.3",
          "transcript_support_level": 1,
          "aa_start": 498,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1493,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": 1666,
          "cdna_end": null,
          "cdna_length": 2415,
          "mane_select": "NM_004490.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263915.8"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1652A>C",
          "hgvs_p": "p.Glu551Ala",
          "transcript": "ENST00000943514.1",
          "protein_id": "ENSP00000613573.1",
          "transcript_support_level": null,
          "aa_start": 551,
          "aa_end": null,
          "aa_length": 593,
          "cds_start": 1652,
          "cds_end": null,
          "cds_length": 1782,
          "cdna_start": 1786,
          "cdna_end": null,
          "cdna_length": 2129,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943514.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1640A>C",
          "hgvs_p": "p.Glu547Ala",
          "transcript": "ENST00000943511.1",
          "protein_id": "ENSP00000613570.1",
          "transcript_support_level": null,
          "aa_start": 547,
          "aa_end": null,
          "aa_length": 589,
          "cds_start": 1640,
          "cds_end": null,
          "cds_length": 1770,
          "cdna_start": 1809,
          "cdna_end": null,
          "cdna_length": 2159,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943511.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1493A>C",
          "hgvs_p": "p.Glu498Ala",
          "transcript": "ENST00000894024.1",
          "protein_id": "ENSP00000564083.1",
          "transcript_support_level": null,
          "aa_start": 498,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1493,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": 1762,
          "cdna_end": null,
          "cdna_length": 2106,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894024.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1439A>C",
          "hgvs_p": "p.Glu480Ala",
          "transcript": "ENST00000911822.1",
          "protein_id": "ENSP00000581881.1",
          "transcript_support_level": null,
          "aa_start": 480,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1439,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": 1580,
          "cdna_end": null,
          "cdna_length": 1923,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911822.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1418A>C",
          "hgvs_p": "p.Glu473Ala",
          "transcript": "ENST00000894027.1",
          "protein_id": "ENSP00000564086.1",
          "transcript_support_level": null,
          "aa_start": 473,
          "aa_end": null,
          "aa_length": 515,
          "cds_start": 1418,
          "cds_end": null,
          "cds_length": 1548,
          "cdna_start": 1560,
          "cdna_end": null,
          "cdna_length": 1903,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894027.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1412A>C",
          "hgvs_p": "p.Glu471Ala",
          "transcript": "ENST00000894025.1",
          "protein_id": "ENSP00000564084.1",
          "transcript_support_level": null,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 1412,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": 1648,
          "cdna_end": null,
          "cdna_length": 1991,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894025.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1397A>C",
          "hgvs_p": "p.Glu466Ala",
          "transcript": "ENST00000894026.1",
          "protein_id": "ENSP00000564085.1",
          "transcript_support_level": null,
          "aa_start": 466,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1397,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1566,
          "cdna_end": null,
          "cdna_length": 1908,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894026.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1358A>C",
          "hgvs_p": "p.Glu453Ala",
          "transcript": "ENST00000943512.1",
          "protein_id": "ENSP00000613571.1",
          "transcript_support_level": null,
          "aa_start": 453,
          "aa_end": null,
          "aa_length": 495,
          "cds_start": 1358,
          "cds_end": null,
          "cds_length": 1488,
          "cdna_start": 1501,
          "cdna_end": null,
          "cdna_length": 1844,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943512.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1316A>C",
          "hgvs_p": "p.Glu439Ala",
          "transcript": "ENST00000911821.1",
          "protein_id": "ENSP00000581880.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 481,
          "cds_start": 1316,
          "cds_end": null,
          "cds_length": 1446,
          "cdna_start": 1471,
          "cdna_end": null,
          "cdna_length": 1821,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911821.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1286A>C",
          "hgvs_p": "p.Glu429Ala",
          "transcript": "ENST00000943513.1",
          "protein_id": "ENSP00000613572.1",
          "transcript_support_level": null,
          "aa_start": 429,
          "aa_end": null,
          "aa_length": 471,
          "cds_start": 1286,
          "cds_end": null,
          "cds_length": 1416,
          "cdna_start": 1428,
          "cdna_end": null,
          "cdna_length": 1771,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943513.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1232A>C",
          "hgvs_p": "p.Glu411Ala",
          "transcript": "NM_001303422.2",
          "protein_id": "NP_001290351.1",
          "transcript_support_level": null,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": 1350,
          "cdna_end": null,
          "cdna_length": 2099,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001303422.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1232A>C",
          "hgvs_p": "p.Glu411Ala",
          "transcript": "ENST00000696453.2",
          "protein_id": "ENSP00000512640.1",
          "transcript_support_level": null,
          "aa_start": 411,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 1232,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": 1350,
          "cdna_end": null,
          "cdna_length": 2099,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000696453.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.893A>C",
          "hgvs_p": "p.Glu298Ala",
          "transcript": "XM_047444013.1",
          "protein_id": "XP_047299969.1",
          "transcript_support_level": null,
          "aa_start": 298,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 893,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": 1226,
          "cdna_end": null,
          "cdna_length": 1975,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047444013.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "n.1629A>C",
          "hgvs_p": null,
          "transcript": "ENST00000488342.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1983,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000488342.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "n.62A>C",
          "hgvs_p": null,
          "transcript": "ENST00000497306.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 406,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000497306.1"
        }
      ],
      "gene_symbol": "GRB14",
      "gene_hgnc_id": 4565,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.18670403957366943,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.124,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.123,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.51,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.148,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004490.3",
          "gene_symbol": "GRB14",
          "hgnc_id": 4565,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1493A>C",
          "hgvs_p": "p.Glu498Ala"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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