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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-164497258-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=164497258&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 164497258,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_004490.3",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1247C>T",
          "hgvs_p": "p.Thr416Ile",
          "transcript": "NM_004490.3",
          "protein_id": "NP_004481.2",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1247,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": 1420,
          "cdna_end": null,
          "cdna_length": 2415,
          "mane_select": "ENST00000263915.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004490.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1247C>T",
          "hgvs_p": "p.Thr416Ile",
          "transcript": "ENST00000263915.8",
          "protein_id": "ENSP00000263915.3",
          "transcript_support_level": 1,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1247,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": 1420,
          "cdna_end": null,
          "cdna_length": 2415,
          "mane_select": "NM_004490.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263915.8"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1112C>T",
          "hgvs_p": "p.Thr371Ile",
          "transcript": "ENST00000446413.6",
          "protein_id": "ENSP00000416786.2",
          "transcript_support_level": 1,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1247,
          "cdna_start": 1300,
          "cdna_end": null,
          "cdna_length": 1435,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000446413.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1406C>T",
          "hgvs_p": "p.Thr469Ile",
          "transcript": "ENST00000943514.1",
          "protein_id": "ENSP00000613573.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 593,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 1782,
          "cdna_start": 1540,
          "cdna_end": null,
          "cdna_length": 2129,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943514.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1394C>T",
          "hgvs_p": "p.Thr465Ile",
          "transcript": "ENST00000943511.1",
          "protein_id": "ENSP00000613570.1",
          "transcript_support_level": null,
          "aa_start": 465,
          "aa_end": null,
          "aa_length": 589,
          "cds_start": 1394,
          "cds_end": null,
          "cds_length": 1770,
          "cdna_start": 1563,
          "cdna_end": null,
          "cdna_length": 2159,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943511.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1247C>T",
          "hgvs_p": "p.Thr416Ile",
          "transcript": "ENST00000894024.1",
          "protein_id": "ENSP00000564083.1",
          "transcript_support_level": null,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1247,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": 1516,
          "cdna_end": null,
          "cdna_length": 2106,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894024.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1172C>T",
          "hgvs_p": "p.Thr391Ile",
          "transcript": "ENST00000894027.1",
          "protein_id": "ENSP00000564086.1",
          "transcript_support_level": null,
          "aa_start": 391,
          "aa_end": null,
          "aa_length": 515,
          "cds_start": 1172,
          "cds_end": null,
          "cds_length": 1548,
          "cdna_start": 1314,
          "cdna_end": null,
          "cdna_length": 1903,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894027.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1166C>T",
          "hgvs_p": "p.Thr389Ile",
          "transcript": "ENST00000894025.1",
          "protein_id": "ENSP00000564084.1",
          "transcript_support_level": null,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 1166,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": 1402,
          "cdna_end": null,
          "cdna_length": 1991,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894025.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1151C>T",
          "hgvs_p": "p.Thr384Ile",
          "transcript": "ENST00000894026.1",
          "protein_id": "ENSP00000564085.1",
          "transcript_support_level": null,
          "aa_start": 384,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1151,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 1320,
          "cdna_end": null,
          "cdna_length": 1908,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000894026.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1112C>T",
          "hgvs_p": "p.Thr371Ile",
          "transcript": "ENST00000943512.1",
          "protein_id": "ENSP00000613571.1",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 495,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1488,
          "cdna_start": 1255,
          "cdna_end": null,
          "cdna_length": 1844,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943512.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1070C>T",
          "hgvs_p": "p.Thr357Ile",
          "transcript": "ENST00000911821.1",
          "protein_id": "ENSP00000581880.1",
          "transcript_support_level": null,
          "aa_start": 357,
          "aa_end": null,
          "aa_length": 481,
          "cds_start": 1070,
          "cds_end": null,
          "cds_length": 1446,
          "cdna_start": 1225,
          "cdna_end": null,
          "cdna_length": 1821,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911821.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1040C>T",
          "hgvs_p": "p.Thr347Ile",
          "transcript": "ENST00000943513.1",
          "protein_id": "ENSP00000613572.1",
          "transcript_support_level": null,
          "aa_start": 347,
          "aa_end": null,
          "aa_length": 471,
          "cds_start": 1040,
          "cds_end": null,
          "cds_length": 1416,
          "cdna_start": 1182,
          "cdna_end": null,
          "cdna_length": 1771,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000943513.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.986C>T",
          "hgvs_p": "p.Thr329Ile",
          "transcript": "NM_001303422.2",
          "protein_id": "NP_001290351.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": 1104,
          "cdna_end": null,
          "cdna_length": 2099,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001303422.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.986C>T",
          "hgvs_p": "p.Thr329Ile",
          "transcript": "ENST00000696453.2",
          "protein_id": "ENSP00000512640.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": 1104,
          "cdna_end": null,
          "cdna_length": 2099,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000696453.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.647C>T",
          "hgvs_p": "p.Thr216Ile",
          "transcript": "XM_047444013.1",
          "protein_id": "XP_047299969.1",
          "transcript_support_level": null,
          "aa_start": 216,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 647,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": 980,
          "cdna_end": null,
          "cdna_length": 1975,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047444013.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 10,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "c.1222-29C>T",
          "hgvs_p": null,
          "transcript": "ENST00000911822.1",
          "protein_id": "ENSP00000581881.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1923,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000911822.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "n.1383C>T",
          "hgvs_p": null,
          "transcript": "ENST00000488342.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1983,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000488342.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRB14",
          "gene_hgnc_id": 4565,
          "hgvs_c": "n.-206C>T",
          "hgvs_p": null,
          "transcript": "ENST00000497306.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 406,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000497306.1"
        }
      ],
      "gene_symbol": "GRB14",
      "gene_hgnc_id": 4565,
      "dbsnp": "rs901611467",
      "frequency_reference_population": 0.0000030988995,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000205295,
      "gnomad_genomes_af": 0.0000131435,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.05279681086540222,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.07,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0886,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.6,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.494,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_004490.3",
          "gene_symbol": "GRB14",
          "hgnc_id": 4565,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.1247C>T",
          "hgvs_p": "p.Thr416Ile"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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