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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-165924678-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=165924678&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 165924678,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_024753.5",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "NM_024753.5",
          "protein_id": "NP_079029.3",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1316,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3951,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000243344.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024753.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "ENST00000243344.8",
          "protein_id": "ENSP00000243344.7",
          "transcript_support_level": 1,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1316,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3951,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024753.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000243344.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "ENST00000679840.1",
          "protein_id": "ENSP00000505248.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1323,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3972,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679840.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "ENST00000679799.1",
          "protein_id": "ENSP00000505208.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1274,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3825,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000679799.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "ENST00000959804.1",
          "protein_id": "ENSP00000629863.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1240,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000959804.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "ENST00000680448.1",
          "protein_id": "ENSP00000505921.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1056,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3171,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000680448.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "ENST00000681606.1",
          "protein_id": "ENSP00000505354.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1004,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3015,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000681606.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "XM_017004967.2",
          "protein_id": "XP_016860456.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1314,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017004967.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.733C>T",
          "hgvs_p": "p.Pro245Ser",
          "transcript": "XM_047445870.1",
          "protein_id": "XP_047301826.1",
          "transcript_support_level": null,
          "aa_start": 245,
          "aa_end": null,
          "aa_length": 1098,
          "cds_start": 733,
          "cds_end": null,
          "cds_length": 3297,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047445870.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.637C>T",
          "hgvs_p": "p.Pro213Ser",
          "transcript": "XM_011511871.4",
          "protein_id": "XP_011510173.1",
          "transcript_support_level": null,
          "aa_start": 213,
          "aa_end": null,
          "aa_length": 1066,
          "cds_start": 637,
          "cds_end": null,
          "cds_length": 3201,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011511871.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "XM_006712761.2",
          "protein_id": "XP_006712824.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 1056,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 3171,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006712761.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser",
          "transcript": "XM_011511872.3",
          "protein_id": "XP_011510174.1",
          "transcript_support_level": null,
          "aa_start": 463,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 1387,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011511872.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "n.1387C>T",
          "hgvs_p": null,
          "transcript": "ENST00000652557.1",
          "protein_id": "ENSP00000498617.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000652557.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "n.1387C>T",
          "hgvs_p": null,
          "transcript": "ENST00000679356.1",
          "protein_id": "ENSP00000506245.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000679356.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "n.1500C>T",
          "hgvs_p": null,
          "transcript": "ENST00000679671.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000679671.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "n.1276C>T",
          "hgvs_p": null,
          "transcript": "ENST00000679676.1",
          "protein_id": "ENSP00000505492.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000679676.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "n.*429C>T",
          "hgvs_p": null,
          "transcript": "ENST00000679931.1",
          "protein_id": "ENSP00000505632.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000679931.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "n.1387C>T",
          "hgvs_p": null,
          "transcript": "ENST00000679967.1",
          "protein_id": "ENSP00000506607.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000679967.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTC21B",
          "gene_hgnc_id": 25660,
          "hgvs_c": "n.646C>T",
          "hgvs_p": null,
          "transcript": "ENST00000680225.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000680225.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 31,
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      "dbsnp": "rs16851307",
      "frequency_reference_population": 0.002397918,
      "hom_count_reference_population": 93,
      "allele_count_reference_population": 3868,
      "gnomad_exomes_af": 0.00128342,
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      "gnomad_exomes_ac": 1875,
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      "gnomad_exomes_homalt": 35,
      "gnomad_genomes_homalt": 58,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.005339890718460083,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.9399999976158142,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.284,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2185,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.37,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.31,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.16,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.995240014161059,
      "dbscsnv_ada_prediction": "Pathogenic",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -15,
      "acmg_classification": "Benign",
      "acmg_criteria": "PP3,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -15,
          "benign_score": 16,
          "pathogenic_score": 1,
          "criteria": [
            "PP3",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_024753.5",
          "gene_symbol": "TTC21B",
          "hgnc_id": 25660,
          "effects": [
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            "splice_region_variant"
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          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1387C>T",
          "hgvs_p": "p.Pro463Ser"
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      ],
      "clinvar_disease": "Asphyxiating thoracic dystrophy 4,Connective tissue disorder,Jeune thoracic dystrophy,Nephronophthisis,Nephronophthisis 12,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:10",
      "phenotype_combined": "not specified|Nephronophthisis 12|not provided|Asphyxiating thoracic dystrophy 4|Nephronophthisis;Jeune thoracic dystrophy|Connective tissue disorder",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}