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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-165992050-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=165992050&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 165992050,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001165963.4",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5225A>G",
          "hgvs_p": "p.Asp1742Gly",
          "transcript": "NM_001165963.4",
          "protein_id": "NP_001159435.1",
          "transcript_support_level": null,
          "aa_start": 1742,
          "aa_end": null,
          "aa_length": 2009,
          "cds_start": 5225,
          "cds_end": null,
          "cds_length": 6030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000674923.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001165963.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5225A>G",
          "hgvs_p": "p.Asp1742Gly",
          "transcript": "ENST00000674923.1",
          "protein_id": "ENSP00000501589.1",
          "transcript_support_level": null,
          "aa_start": 1742,
          "aa_end": null,
          "aa_length": 2009,
          "cds_start": 5225,
          "cds_end": null,
          "cds_length": 6030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001165963.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674923.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5225A>G",
          "hgvs_p": "p.Asp1742Gly",
          "transcript": "ENST00000303395.9",
          "protein_id": "ENSP00000303540.4",
          "transcript_support_level": 5,
          "aa_start": 1742,
          "aa_end": null,
          "aa_length": 2009,
          "cds_start": 5225,
          "cds_end": null,
          "cds_length": 6030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000303395.9"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "ENST00000375405.7",
          "protein_id": "ENSP00000364554.3",
          "transcript_support_level": 5,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375405.7"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5141A>G",
          "hgvs_p": "p.Asp1714Gly",
          "transcript": "ENST00000409050.2",
          "protein_id": "ENSP00000386312.1",
          "transcript_support_level": 5,
          "aa_start": 1714,
          "aa_end": null,
          "aa_length": 1981,
          "cds_start": 5141,
          "cds_end": null,
          "cds_length": 5946,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409050.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5225A>G",
          "hgvs_p": "p.Asp1742Gly",
          "transcript": "NM_001202435.3",
          "protein_id": "NP_001189364.1",
          "transcript_support_level": null,
          "aa_start": 1742,
          "aa_end": null,
          "aa_length": 2009,
          "cds_start": 5225,
          "cds_end": null,
          "cds_length": 6030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001202435.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5225A>G",
          "hgvs_p": "p.Asp1742Gly",
          "transcript": "NM_001353948.2",
          "protein_id": "NP_001340877.1",
          "transcript_support_level": null,
          "aa_start": 1742,
          "aa_end": null,
          "aa_length": 2009,
          "cds_start": 5225,
          "cds_end": null,
          "cds_length": 6030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353948.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "NM_001353949.2",
          "protein_id": "NP_001340878.1",
          "transcript_support_level": null,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353949.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "NM_001353950.2",
          "protein_id": "NP_001340879.1",
          "transcript_support_level": null,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353950.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "NM_001353951.2",
          "protein_id": "NP_001340880.1",
          "transcript_support_level": null,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353951.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "NM_001353952.2",
          "protein_id": "NP_001340881.1",
          "transcript_support_level": null,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001353952.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "NM_006920.6",
          "protein_id": "NP_008851.3",
          "transcript_support_level": null,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_006920.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "ENST00000635750.1",
          "protein_id": "ENSP00000490799.1",
          "transcript_support_level": 5,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000635750.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "ENST00000637988.1",
          "protein_id": "ENSP00000490780.1",
          "transcript_support_level": 5,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000637988.1"
        },
        {
          "aa_ref": "D",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5192A>G",
          "hgvs_p": "p.Asp1731Gly",
          "transcript": "ENST00000640036.1",
          "protein_id": "ENSP00000491573.1",
          "transcript_support_level": 5,
          "aa_start": 1731,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 5192,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000640036.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5189A>G",
          "hgvs_p": "p.Asp1730Gly",
          "transcript": "NM_001353954.2",
          "protein_id": "NP_001340883.1",
          "transcript_support_level": null,
          "aa_start": 1730,
          "aa_end": null,
          "aa_length": 1997,
          "cds_start": 5189,
          "cds_end": null,
          "cds_length": 5994,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "NM_001353954.2"
        },
        {
          "aa_ref": "D",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5189A>G",
          "hgvs_p": "p.Asp1730Gly",
          "transcript": "NM_001353955.2",
          "protein_id": "NP_001340884.1",
          "transcript_support_level": null,
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          "aa_end": null,
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          "biotype": "protein_coding",
          "feature": "NM_001353955.2"
        },
        {
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          "protein_coding": true,
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          "consequences": [
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          ],
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          "exon_count": 28,
          "intron_rank": null,
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          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5189A>G",
          "hgvs_p": "p.Asp1730Gly",
          "transcript": "ENST00000641575.1",
          "protein_id": "ENSP00000492917.1",
          "transcript_support_level": null,
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          "aa_length": 1997,
          "cds_start": 5189,
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        },
        {
          "aa_ref": "D",
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          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5141A>G",
          "hgvs_p": "p.Asp1714Gly",
          "transcript": "NM_001165964.3",
          "protein_id": "NP_001159436.1",
          "transcript_support_level": null,
          "aa_start": 1714,
          "aa_end": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001165964.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "G",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SCN1A",
          "gene_hgnc_id": 10585,
          "hgvs_c": "c.5141A>G",
          "hgvs_p": "p.Asp1714Gly",
          "transcript": "NM_001353957.2",
          "protein_id": "NP_001340886.1",
          "transcript_support_level": null,
          "aa_start": 1714,
          "aa_end": null,
          "aa_length": 1981,
          "cds_start": 5141,
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          "gene_symbol": "SCN1A-AS1",
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          "hgvs_c": "n.406-23563T>C",
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          "transcript": "ENST00000671284.1",
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        {
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          "biotype": "pseudogene",
          "feature": "NR_110598.1"
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      ],
      "gene_symbol": "SCN1A",
      "gene_hgnc_id": 10585,
      "dbsnp": "rs121918812",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8862313032150269,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.931,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.8523,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.5,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 6.125,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 9,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 9,
          "benign_score": 0,
          "pathogenic_score": 9,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP2",
            "PP3_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001165963.4",
          "gene_symbol": "SCN1A",
          "hgnc_id": 10585,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,Unknown",
          "hgvs_c": "c.5225A>G",
          "hgvs_p": "p.Asp1742Gly"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000597623.4",
          "gene_symbol": "SCN1A-AS1",
          "hgnc_id": 54069,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.202-23563T>C",
          "hgvs_p": null
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_110598.1",
          "gene_symbol": "LOC102724058",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.176-23563T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " type 1,Generalized epilepsy with febrile seizures plus",
      "clinvar_classification": "not provided",
      "clinvar_review_status": "no classification provided",
      "clinvar_submissions_summary": "O:1",
      "phenotype_combined": "Generalized epilepsy with febrile seizures plus, type 1",
      "pathogenicity_classification_combined": "not provided",
      "custom_annotations": null
    }
  ],
  "message": null
}