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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-168968473-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=168968473&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 168968473,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000650372.1",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.2029A>G",
          "hgvs_p": "p.Met677Val",
          "transcript": "NM_003742.4",
          "protein_id": "NP_003733.2",
          "transcript_support_level": null,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 1321,
          "cds_start": 2029,
          "cds_end": null,
          "cds_length": 3966,
          "cdna_start": 2156,
          "cdna_end": null,
          "cdna_length": 6934,
          "mane_select": "ENST00000650372.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.2029A>G",
          "hgvs_p": "p.Met677Val",
          "transcript": "ENST00000650372.1",
          "protein_id": "ENSP00000497931.1",
          "transcript_support_level": null,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 1321,
          "cds_start": 2029,
          "cds_end": null,
          "cds_length": 3966,
          "cdna_start": 2156,
          "cdna_end": null,
          "cdna_length": 6934,
          "mane_select": "NM_003742.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.346A>G",
          "hgvs_p": "p.Met116Val",
          "transcript": "ENST00000649448.1",
          "protein_id": "ENSP00000497165.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 780,
          "cds_start": 346,
          "cds_end": null,
          "cds_length": 2343,
          "cdna_start": 346,
          "cdna_end": null,
          "cdna_length": 2983,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.2131A>G",
          "hgvs_p": "p.Met711Val",
          "transcript": "XM_011512078.3",
          "protein_id": "XP_011510380.1",
          "transcript_support_level": null,
          "aa_start": 711,
          "aa_end": null,
          "aa_length": 1355,
          "cds_start": 2131,
          "cds_end": null,
          "cds_length": 4068,
          "cdna_start": 2258,
          "cdna_end": null,
          "cdna_length": 4974,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.2071A>G",
          "hgvs_p": "p.Met691Val",
          "transcript": "XM_006712817.4",
          "protein_id": "XP_006712880.1",
          "transcript_support_level": null,
          "aa_start": 691,
          "aa_end": null,
          "aa_length": 1335,
          "cds_start": 2071,
          "cds_end": null,
          "cds_length": 4008,
          "cdna_start": 2198,
          "cdna_end": null,
          "cdna_length": 4181,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.2131A>G",
          "hgvs_p": "p.Met711Val",
          "transcript": "XM_017005165.2",
          "protein_id": "XP_016860654.1",
          "transcript_support_level": null,
          "aa_start": 711,
          "aa_end": null,
          "aa_length": 1314,
          "cds_start": 2131,
          "cds_end": null,
          "cds_length": 3945,
          "cdna_start": 2258,
          "cdna_end": null,
          "cdna_length": 4287,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.1360A>G",
          "hgvs_p": "p.Met454Val",
          "transcript": "XM_017005166.2",
          "protein_id": "XP_016860655.1",
          "transcript_support_level": null,
          "aa_start": 454,
          "aa_end": null,
          "aa_length": 1098,
          "cds_start": 1360,
          "cds_end": null,
          "cds_length": 3297,
          "cdna_start": 1453,
          "cdna_end": null,
          "cdna_length": 3436,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.2131A>G",
          "hgvs_p": "p.Met711Val",
          "transcript": "XM_011512080.3",
          "protein_id": "XP_011510382.1",
          "transcript_support_level": null,
          "aa_start": 711,
          "aa_end": null,
          "aa_length": 936,
          "cds_start": 2131,
          "cds_end": null,
          "cds_length": 2811,
          "cdna_start": 2258,
          "cdna_end": null,
          "cdna_length": 3053,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "c.814A>G",
          "hgvs_p": "p.Met272Val",
          "transcript": "XM_017005167.2",
          "protein_id": "XP_016860656.1",
          "transcript_support_level": null,
          "aa_start": 272,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 814,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": 1010,
          "cdna_end": null,
          "cdna_length": 2993,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "n.*499A>G",
          "hgvs_p": null,
          "transcript": "ENST00000439188.1",
          "protein_id": "ENSP00000416058.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2822,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ABCB11",
          "gene_hgnc_id": 42,
          "hgvs_c": "n.*499A>G",
          "hgvs_p": null,
          "transcript": "ENST00000439188.1",
          "protein_id": "ENSP00000416058.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2822,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "ABCB11",
      "gene_hgnc_id": 42,
      "dbsnp": "rs11568364",
      "frequency_reference_population": 0.023484617,
      "hom_count_reference_population": 1425,
      "allele_count_reference_population": 37834,
      "gnomad_exomes_af": 0.0198946,
      "gnomad_genomes_af": 0.0579397,
      "gnomad_exomes_ac": 29026,
      "gnomad_genomes_ac": 8808,
      "gnomad_exomes_homalt": 799,
      "gnomad_genomes_homalt": 626,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0014809668064117432,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.20000000298023224,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.211,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0538,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.57,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.037,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.2,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000650372.1",
          "gene_symbol": "ABCB11",
          "hgnc_id": 42,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2029A>G",
          "hgvs_p": "p.Met677Val"
        }
      ],
      "clinvar_disease": "Progressive familial intrahepatic cholestasis type 2,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2 B:4",
      "phenotype_combined": "not specified|Progressive familial intrahepatic cholestasis type 2|not provided",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}