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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-171522589-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=171522589&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 171522589,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_024843.4",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Ser15Leu",
          "transcript": "NM_024843.4",
          "protein_id": "NP_079119.3",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 286,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 861,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000321348.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024843.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Ser15Leu",
          "transcript": "ENST00000321348.9",
          "protein_id": "ENSP00000319141.4",
          "transcript_support_level": 1,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 286,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 861,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024843.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000321348.9"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Ser15Leu",
          "transcript": "ENST00000375252.3",
          "protein_id": "ENSP00000364401.3",
          "transcript_support_level": 1,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 157,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 474,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375252.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Ser15Leu",
          "transcript": "ENST00000858692.1",
          "protein_id": "ENSP00000528751.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 285,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 858,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858692.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Ser15Leu",
          "transcript": "NM_001127383.2",
          "protein_id": "NP_001120855.1",
          "transcript_support_level": null,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 157,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 474,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001127383.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "c.19+298C>T",
          "hgvs_p": null,
          "transcript": "NM_001256909.2",
          "protein_id": "NP_001243838.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256909.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "c.19+298C>T",
          "hgvs_p": null,
          "transcript": "ENST00000409484.5",
          "protein_id": "ENSP00000386739.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409484.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "n.242C>T",
          "hgvs_p": null,
          "transcript": "ENST00000468308.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000468308.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "n.80C>T",
          "hgvs_p": null,
          "transcript": "ENST00000474182.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000474182.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYBRD1",
          "gene_hgnc_id": 20797,
          "hgvs_c": "n.79C>T",
          "hgvs_p": null,
          "transcript": "ENST00000494587.1",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000494587.1"
        }
      ],
      "gene_symbol": "CYBRD1",
      "gene_hgnc_id": 20797,
      "dbsnp": "rs146713767",
      "frequency_reference_population": 0.0015283081,
      "hom_count_reference_population": 4,
      "allele_count_reference_population": 2463,
      "gnomad_exomes_af": 0.00158843,
      "gnomad_genomes_af": 0.000952156,
      "gnomad_exomes_ac": 2318,
      "gnomad_genomes_ac": 145,
      "gnomad_exomes_homalt": 3,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.00937393307685852,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.083,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1456,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.34,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.015,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -9,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6,BS2",
      "acmg_by_gene": [
        {
          "score": -9,
          "benign_score": 9,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_024843.4",
          "gene_symbol": "CYBRD1",
          "hgnc_id": 20797,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Ser15Leu"
        }
      ],
      "clinvar_disease": "CYBRD1-related disorder",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "CYBRD1-related disorder",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}