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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-178559329-CCTTT-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=178559329&ref=CCTTT&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 178559329,
      "ref": "CCTTT",
      "alt": "C",
      "effect": "frameshift_variant",
      "transcript": "ENST00000589042.5",
      "consequences": [
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 326,
          "exon_rank_end": null,
          "exon_count": 363,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86799_86802delAAAG",
          "hgvs_p": "p.Gly28936fs",
          "transcript": "NM_001267550.2",
          "protein_id": "NP_001254479.2",
          "transcript_support_level": null,
          "aa_start": 28933,
          "aa_end": null,
          "aa_length": 35991,
          "cds_start": 86799,
          "cds_end": null,
          "cds_length": 107976,
          "cdna_start": 87027,
          "cdna_end": null,
          "cdna_length": 109224,
          "mane_select": "ENST00000589042.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 326,
          "exon_rank_end": null,
          "exon_count": 363,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86799_86802delAAAG",
          "hgvs_p": "p.Gly28936fs",
          "transcript": "ENST00000589042.5",
          "protein_id": "ENSP00000467141.1",
          "transcript_support_level": 5,
          "aa_start": 28933,
          "aa_end": null,
          "aa_length": 35991,
          "cds_start": 86799,
          "cds_end": null,
          "cds_length": 107976,
          "cdna_start": 87027,
          "cdna_end": null,
          "cdna_length": 109224,
          "mane_select": "NM_001267550.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 324,
          "exon_rank_end": null,
          "exon_count": 361,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86643_86646delAAAG",
          "hgvs_p": "p.Gly28884fs",
          "transcript": "ENST00000446966.2",
          "protein_id": "ENSP00000408004.2",
          "transcript_support_level": 1,
          "aa_start": 28881,
          "aa_end": null,
          "aa_length": 35939,
          "cds_start": 86643,
          "cds_end": null,
          "cds_length": 107820,
          "cdna_start": 86871,
          "cdna_end": null,
          "cdna_length": 109068,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 324,
          "exon_rank_end": null,
          "exon_count": 361,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86523_86526delAAAG",
          "hgvs_p": "p.Gly28844fs",
          "transcript": "ENST00000436599.2",
          "protein_id": "ENSP00000405517.2",
          "transcript_support_level": 1,
          "aa_start": 28841,
          "aa_end": null,
          "aa_length": 35899,
          "cds_start": 86523,
          "cds_end": null,
          "cds_length": 107700,
          "cdna_start": 86751,
          "cdna_end": null,
          "cdna_length": 108948,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 319,
          "exon_rank_end": null,
          "exon_count": 356,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86241_86244delAAAG",
          "hgvs_p": "p.Gly28750fs",
          "transcript": "ENST00000426232.6",
          "protein_id": "ENSP00000392336.2",
          "transcript_support_level": 1,
          "aa_start": 28747,
          "aa_end": null,
          "aa_length": 35805,
          "cds_start": 86241,
          "cds_end": null,
          "cds_length": 107418,
          "cdna_start": 86469,
          "cdna_end": null,
          "cdna_length": 108666,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "TTN-AS1",
          "gene_hgnc_id": 44124,
          "hgvs_c": "n.2043+16969_2043+16972delCTTT",
          "hgvs_p": null,
          "transcript": "ENST00000419746.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4592,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 328,
          "exon_rank_end": null,
          "exon_count": 365,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86799_86802delAAAG",
          "hgvs_p": "p.Gly28936fs",
          "transcript": "ENST00000412264.2",
          "protein_id": "ENSP00000394672.2",
          "transcript_support_level": 3,
          "aa_start": 28933,
          "aa_end": null,
          "aa_length": 35991,
          "cds_start": 86799,
          "cds_end": null,
          "cds_length": 107976,
          "cdna_start": 87291,
          "cdna_end": null,
          "cdna_length": 109488,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 325,
          "exon_rank_end": null,
          "exon_count": 362,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86715_86718delAAAG",
          "hgvs_p": "p.Gly28908fs",
          "transcript": "ENST00000425332.3",
          "protein_id": "ENSP00000396805.3",
          "transcript_support_level": 5,
          "aa_start": 28905,
          "aa_end": null,
          "aa_length": 35963,
          "cds_start": 86715,
          "cds_end": null,
          "cds_length": 107892,
          "cdna_start": 86943,
          "cdna_end": null,
          "cdna_length": 109140,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "TK",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 323,
          "exon_rank_end": null,
          "exon_count": 360,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.86385_86388delAAAG",
          "hgvs_p": "p.Gly28798fs",
          "transcript": "ENST00000715174.1",
          "protein_id": "ENSP00000520370.1",
          "transcript_support_level": null,
          "aa_start": 28795,
          "aa_end": null,
          "aa_length": 35853,
          "cds_start": 86385,
          "cds_end": null,
          "cds_length": 107562,
          "cdna_start": 86613,
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          "cdna_length": 108810,
          "mane_select": null,
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          "biotype": null,
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 276,
          "exon_rank_end": null,
          "exon_count": 313,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.81876_81879delAAAG",
          "hgvs_p": "p.Gly27295fs",
          "transcript": "NM_001256850.1",
          "protein_id": "NP_001243779.1",
          "transcript_support_level": null,
          "aa_start": 27292,
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          "aa_length": 34350,
          "cds_start": 81876,
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          "cdna_start": 82104,
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          "mane_select": null,
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        },
        {
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          "exon_rank": 276,
          "exon_rank_end": null,
          "exon_count": 313,
          "intron_rank": null,
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          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.81876_81879delAAAG",
          "hgvs_p": "p.Gly27295fs",
          "transcript": "ENST00000591111.5",
          "protein_id": "ENSP00000465570.1",
          "transcript_support_level": 5,
          "aa_start": 27292,
          "aa_end": null,
          "aa_length": 34350,
          "cds_start": 81876,
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        },
        {
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          "consequences": [
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          "exon_rank": 275,
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          "exon_count": 312,
          "intron_rank": null,
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          "gene_symbol": "TTN",
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          "hgvs_c": "c.79095_79098delAAAG",
          "hgvs_p": "p.Gly26368fs",
          "transcript": "NM_133378.4",
          "protein_id": "NP_596869.4",
          "transcript_support_level": null,
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        {
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          "exon_count": 312,
          "intron_rank": null,
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          "gene_symbol": "TTN",
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          "hgvs_c": "c.79095_79098delAAAG",
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          "transcript": "ENST00000342992.11",
          "protein_id": "ENSP00000343764.6",
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        {
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          "exon_rank_end": null,
          "exon_count": 192,
          "intron_rank": null,
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          "gene_symbol": "TTN",
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          "hgvs_c": "c.60180_60183delAAAG",
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          "transcript": "NM_133437.4",
          "protein_id": "NP_597681.4",
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        {
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          "exon_count": 192,
          "intron_rank": null,
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          "gene_symbol": "TTN",
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          "hgvs_c": "c.59979_59982delAAAG",
          "hgvs_p": "p.Gly19996fs",
          "transcript": "NM_133432.3",
          "protein_id": "NP_597676.3",
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          "transcript": "ENST00000359218.11",
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        {
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          "consequences": [
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          "exon_count": 359,
          "intron_rank": null,
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          "gene_symbol": "TTN",
          "gene_hgnc_id": 12403,
          "hgvs_c": "c.85692_85695delAAAG",
          "hgvs_p": "p.Gly28567fs",
          "transcript": "XM_017004819.1",
          "protein_id": "XP_016860308.1",
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        {
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        },
        {
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          "hgvs_c": "n.447-11970_447-11967delCTTT",
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        {
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          "exon_count": 17,
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          "gene_symbol": "TTN-AS1",
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          "hgvs_c": "n.2043+16969_2043+16972delCTTT",
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          "cdna_length": 4592,
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        }
      ],
      "gene_symbol": "TTN",
      "gene_hgnc_id": 12403,
      "dbsnp": "rs727504856",
      "frequency_reference_population": 6.939847e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.93985e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 5.052,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000589042.5",
          "gene_symbol": "TTN",
          "hgnc_id": 12403,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.86799_86802delAAAG",
          "hgvs_p": "p.Gly28936fs"
        },
        {
          "score": 10,
          "benign_score": 0,
          "pathogenic_score": 10,
          "criteria": [
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000419746.5",
          "gene_symbol": "TTN-AS1",
          "hgnc_id": 44124,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.2043+16969_2043+16972delCTTT",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Autosomal recessive limb-girdle muscular dystrophy type 2J,Cardiovascular phenotype,Dilated cardiomyopathy 1G,Primary dilated cardiomyopathy,Primary familial dilated cardiomyopathy,TTN-related disorder,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:5 LP:3",
      "phenotype_combined": "Primary dilated cardiomyopathy|not provided|Dilated cardiomyopathy 1G;Autosomal recessive limb-girdle muscular dystrophy type 2J|Primary familial dilated cardiomyopathy|Cardiovascular phenotype|TTN-related disorder",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}