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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-182928136-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=182928136&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 182928136,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_205842.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3161G>A",
          "hgvs_p": "p.Arg1054His",
          "transcript": "NM_013436.5",
          "protein_id": "NP_038464.1",
          "transcript_support_level": null,
          "aa_start": 1054,
          "aa_end": null,
          "aa_length": 1128,
          "cds_start": 3161,
          "cds_end": null,
          "cds_length": 3387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000361354.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_013436.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3161G>A",
          "hgvs_p": "p.Arg1054His",
          "transcript": "ENST00000361354.9",
          "protein_id": "ENSP00000355348.3",
          "transcript_support_level": 1,
          "aa_start": 1054,
          "aa_end": null,
          "aa_length": 1128,
          "cds_start": 3161,
          "cds_end": null,
          "cds_length": 3387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_013436.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361354.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3179G>A",
          "hgvs_p": "p.Arg1060His",
          "transcript": "ENST00000360982.2",
          "protein_id": "ENSP00000354251.2",
          "transcript_support_level": 1,
          "aa_start": 1060,
          "aa_end": null,
          "aa_length": 1134,
          "cds_start": 3179,
          "cds_end": null,
          "cds_length": 3405,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360982.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3179G>A",
          "hgvs_p": "p.Arg1060His",
          "transcript": "NM_205842.3",
          "protein_id": "NP_995314.1",
          "transcript_support_level": null,
          "aa_start": 1060,
          "aa_end": null,
          "aa_length": 1134,
          "cds_start": 3179,
          "cds_end": null,
          "cds_length": 3405,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_205842.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3176G>A",
          "hgvs_p": "p.Arg1059His",
          "transcript": "ENST00000888539.1",
          "protein_id": "ENSP00000558598.1",
          "transcript_support_level": null,
          "aa_start": 1059,
          "aa_end": null,
          "aa_length": 1133,
          "cds_start": 3176,
          "cds_end": null,
          "cds_length": 3402,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888539.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3173G>A",
          "hgvs_p": "p.Arg1058His",
          "transcript": "NM_001437267.1",
          "protein_id": "NP_001424196.1",
          "transcript_support_level": null,
          "aa_start": 1058,
          "aa_end": null,
          "aa_length": 1132,
          "cds_start": 3173,
          "cds_end": null,
          "cds_length": 3399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001437267.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3173G>A",
          "hgvs_p": "p.Arg1058His",
          "transcript": "ENST00000703824.1",
          "protein_id": "ENSP00000515489.1",
          "transcript_support_level": null,
          "aa_start": 1058,
          "aa_end": null,
          "aa_length": 1132,
          "cds_start": 3173,
          "cds_end": null,
          "cds_length": 3399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000703824.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3155G>A",
          "hgvs_p": "p.Arg1052His",
          "transcript": "NM_001437266.1",
          "protein_id": "NP_001424195.1",
          "transcript_support_level": null,
          "aa_start": 1052,
          "aa_end": null,
          "aa_length": 1126,
          "cds_start": 3155,
          "cds_end": null,
          "cds_length": 3381,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001437266.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3155G>A",
          "hgvs_p": "p.Arg1052His",
          "transcript": "ENST00000703825.1",
          "protein_id": "ENSP00000515490.1",
          "transcript_support_level": null,
          "aa_start": 1052,
          "aa_end": null,
          "aa_length": 1126,
          "cds_start": 3155,
          "cds_end": null,
          "cds_length": 3381,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000703825.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3155G>A",
          "hgvs_p": "p.Arg1052His",
          "transcript": "ENST00000939370.1",
          "protein_id": "ENSP00000609429.1",
          "transcript_support_level": null,
          "aa_start": 1052,
          "aa_end": null,
          "aa_length": 1126,
          "cds_start": 3155,
          "cds_end": null,
          "cds_length": 3381,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939370.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3155G>A",
          "hgvs_p": "p.Arg1052His",
          "transcript": "ENST00000939372.1",
          "protein_id": "ENSP00000609431.1",
          "transcript_support_level": null,
          "aa_start": 1052,
          "aa_end": null,
          "aa_length": 1126,
          "cds_start": 3155,
          "cds_end": null,
          "cds_length": 3381,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939372.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 29,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3140G>A",
          "hgvs_p": "p.Arg1047His",
          "transcript": "ENST00000966159.1",
          "protein_id": "ENSP00000636218.1",
          "transcript_support_level": null,
          "aa_start": 1047,
          "aa_end": null,
          "aa_length": 1121,
          "cds_start": 3140,
          "cds_end": null,
          "cds_length": 3366,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966159.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3104G>A",
          "hgvs_p": "p.Arg1035His",
          "transcript": "ENST00000888538.1",
          "protein_id": "ENSP00000558597.1",
          "transcript_support_level": null,
          "aa_start": 1035,
          "aa_end": null,
          "aa_length": 1109,
          "cds_start": 3104,
          "cds_end": null,
          "cds_length": 3330,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888538.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3098G>A",
          "hgvs_p": "p.Arg1033His",
          "transcript": "ENST00000966167.1",
          "protein_id": "ENSP00000636226.1",
          "transcript_support_level": null,
          "aa_start": 1033,
          "aa_end": null,
          "aa_length": 1107,
          "cds_start": 3098,
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          "cds_length": 3324,
          "cdna_start": null,
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          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966167.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3062G>A",
          "hgvs_p": "p.Arg1021His",
          "transcript": "ENST00000888537.1",
          "protein_id": "ENSP00000558596.1",
          "transcript_support_level": null,
          "aa_start": 1021,
          "aa_end": null,
          "aa_length": 1095,
          "cds_start": 3062,
          "cds_end": null,
          "cds_length": 3288,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888537.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3050G>A",
          "hgvs_p": "p.Arg1017His",
          "transcript": "ENST00000888541.1",
          "protein_id": "ENSP00000558600.1",
          "transcript_support_level": null,
          "aa_start": 1017,
          "aa_end": null,
          "aa_length": 1091,
          "cds_start": 3050,
          "cds_end": null,
          "cds_length": 3276,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000888541.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3035G>A",
          "hgvs_p": "p.Arg1012His",
          "transcript": "ENST00000966160.1",
          "protein_id": "ENSP00000636219.1",
          "transcript_support_level": null,
          "aa_start": 1012,
          "aa_end": null,
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          "cds_start": 3035,
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          "cds_length": 3261,
          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
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          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3023G>A",
          "hgvs_p": "p.Arg1008His",
          "transcript": "ENST00000888540.1",
          "protein_id": "ENSP00000558599.1",
          "transcript_support_level": null,
          "aa_start": 1008,
          "aa_end": null,
          "aa_length": 1082,
          "cds_start": 3023,
          "cds_end": null,
          "cds_length": 3249,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000888540.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
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          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.3017G>A",
          "hgvs_p": "p.Arg1006His",
          "transcript": "ENST00000966162.1",
          "protein_id": "ENSP00000636221.1",
          "transcript_support_level": null,
          "aa_start": 1006,
          "aa_end": null,
          "aa_length": 1080,
          "cds_start": 3017,
          "cds_end": null,
          "cds_length": 3243,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966162.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NCKAP1",
          "gene_hgnc_id": 7666,
          "hgvs_c": "c.2942G>A",
          "hgvs_p": "p.Arg981His",
          "transcript": "ENST00000888536.1",
          "protein_id": "ENSP00000558595.1",
          "transcript_support_level": null,
          "aa_start": 981,
          "aa_end": null,
          "aa_length": 1055,
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        {
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          "consequences": [
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          "gene_symbol": "NCKAP1",
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          "hgvs_c": "n.696G>A",
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          "transcript": "ENST00000478449.5",
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          "biotype": "pseudogene",
          "feature": "ENST00000478449.5"
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      ],
      "gene_symbol": "NCKAP1",
      "gene_hgnc_id": 7666,
      "dbsnp": "rs376392398",
      "frequency_reference_population": 0.0000043554655,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 7,
      "gnomad_exomes_af": 0.00000412328,
      "gnomad_genomes_af": 0.00000657791,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.30054372549057007,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.2,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.9719,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.19,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.175,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_205842.3",
          "gene_symbol": "NCKAP1",
          "hgnc_id": 7666,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.3179G>A",
          "hgvs_p": "p.Arg1060His"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Inborn genetic diseases|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}