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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-196993654-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=196993654&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 196993654,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001367495.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "NM_001195144.2",
          "protein_id": "NP_001182073.1",
          "transcript_support_level": null,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 993,
          "cds_start": 2852,
          "cds_end": null,
          "cds_length": 2982,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000282272.15",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001195144.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "ENST00000282272.15",
          "protein_id": "ENSP00000282272.9",
          "transcript_support_level": 5,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 993,
          "cds_start": 2852,
          "cds_end": null,
          "cds_length": 2982,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001195144.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000282272.15"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2297G>A",
          "hgvs_p": "p.Arg766His",
          "transcript": "ENST00000424317.5",
          "protein_id": "ENSP00000403415.1",
          "transcript_support_level": 1,
          "aa_start": 766,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 2297,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000424317.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2906G>A",
          "hgvs_p": "p.Arg969His",
          "transcript": "NM_001367495.1",
          "protein_id": "NP_001354424.1",
          "transcript_support_level": null,
          "aa_start": 969,
          "aa_end": null,
          "aa_length": 1074,
          "cds_start": 2906,
          "cds_end": null,
          "cds_length": 3225,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367495.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "NM_001367497.1",
          "protein_id": "NP_001354426.1",
          "transcript_support_level": null,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 1056,
          "cds_start": 2852,
          "cds_end": null,
          "cds_length": 3171,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367497.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "ENST00000647377.1",
          "protein_id": "ENSP00000496628.1",
          "transcript_support_level": null,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 1056,
          "cds_start": 2852,
          "cds_end": null,
          "cds_length": 3171,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000647377.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2906G>A",
          "hgvs_p": "p.Arg969His",
          "transcript": "ENST00000871701.1",
          "protein_id": "ENSP00000541760.1",
          "transcript_support_level": null,
          "aa_start": 969,
          "aa_end": null,
          "aa_length": 1011,
          "cds_start": 2906,
          "cds_end": null,
          "cds_length": 3036,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000871701.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "NM_001367496.1",
          "protein_id": "NP_001354425.1",
          "transcript_support_level": null,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": 2852,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001367496.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2831G>A",
          "hgvs_p": "p.Arg944His",
          "transcript": "XM_024453216.2",
          "protein_id": "XP_024308984.1",
          "transcript_support_level": null,
          "aa_start": 944,
          "aa_end": null,
          "aa_length": 1049,
          "cds_start": 2831,
          "cds_end": null,
          "cds_length": 3150,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024453216.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2831G>A",
          "hgvs_p": "p.Arg944His",
          "transcript": "XM_047446282.1",
          "protein_id": "XP_047302238.1",
          "transcript_support_level": null,
          "aa_start": 944,
          "aa_end": null,
          "aa_length": 1049,
          "cds_start": 2831,
          "cds_end": null,
          "cds_length": 3150,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047446282.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2777G>A",
          "hgvs_p": "p.Arg926His",
          "transcript": "XM_047446283.1",
          "protein_id": "XP_047302239.1",
          "transcript_support_level": null,
          "aa_start": 926,
          "aa_end": null,
          "aa_length": 1031,
          "cds_start": 2777,
          "cds_end": null,
          "cds_length": 3096,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047446283.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2906G>A",
          "hgvs_p": "p.Arg969His",
          "transcript": "XM_047446284.1",
          "protein_id": "XP_047302240.1",
          "transcript_support_level": null,
          "aa_start": 969,
          "aa_end": null,
          "aa_length": 1023,
          "cds_start": 2906,
          "cds_end": null,
          "cds_length": 3072,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047446284.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2906G>A",
          "hgvs_p": "p.Arg969His",
          "transcript": "XM_005246947.3",
          "protein_id": "XP_005247004.1",
          "transcript_support_level": null,
          "aa_start": 969,
          "aa_end": null,
          "aa_length": 1011,
          "cds_start": 2906,
          "cds_end": null,
          "cds_length": 3036,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005246947.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "XM_047446285.1",
          "protein_id": "XP_047302241.1",
          "transcript_support_level": null,
          "aa_start": 951,
          "aa_end": null,
          "aa_length": 1005,
          "cds_start": 2852,
          "cds_end": null,
          "cds_length": 3018,
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          "feature": "XM_047446285.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 27,
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          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2906G>A",
          "hgvs_p": "p.Arg969His",
          "transcript": "XM_006712838.4",
          "protein_id": "XP_006712901.1",
          "transcript_support_level": null,
          "aa_start": 969,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 2906,
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          "cds_length": 2994,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006712838.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2906G>A",
          "hgvs_p": "p.Arg969His",
          "transcript": "XM_005246948.3",
          "protein_id": "XP_005247005.1",
          "transcript_support_level": null,
          "aa_start": 969,
          "aa_end": null,
          "aa_length": 994,
          "cds_start": 2906,
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          "cds_length": 2985,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "XM_005246948.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "XM_047446286.1",
          "protein_id": "XP_047302242.1",
          "transcript_support_level": null,
          "aa_start": 951,
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          "aa_length": 979,
          "cds_start": 2852,
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          "biotype": "protein_coding",
          "feature": "XM_047446286.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "c.2852G>A",
          "hgvs_p": "p.Arg951His",
          "transcript": "XM_047446287.1",
          "protein_id": "XP_047302243.1",
          "transcript_support_level": null,
          "aa_start": 951,
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          "cds_start": 2852,
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          "cds_length": 2931,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_047446287.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "n.3164G>A",
          "hgvs_p": null,
          "transcript": "NR_160034.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_160034.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKRD44",
          "gene_hgnc_id": 25259,
          "hgvs_c": "n.-16G>A",
          "hgvs_p": null,
          "transcript": "ENST00000486006.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000486006.1"
        }
      ],
      "gene_symbol": "ANKRD44",
      "gene_hgnc_id": 25259,
      "dbsnp": "rs767303463",
      "frequency_reference_population": 0.000037396192,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 58,
      "gnomad_exomes_af": 0.0000400389,
      "gnomad_genomes_af": 0.0000131303,
      "gnomad_exomes_ac": 56,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.10937973856925964,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.237,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.157,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.14,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.872,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001367495.1",
          "gene_symbol": "ANKRD44",
          "hgnc_id": 25259,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.2906G>A",
          "hgvs_p": "p.Arg969His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}