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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-200881474-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=200881474&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 200881474,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_032472.4",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.187G>A",
          "hgvs_p": "p.Gly63Ser",
          "transcript": "NM_130906.3",
          "protein_id": "NP_570981.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 187,
          "cds_end": null,
          "cds_length": 486,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000392283.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_130906.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.187G>A",
          "hgvs_p": "p.Gly63Ser",
          "transcript": "ENST00000392283.9",
          "protein_id": "ENSP00000376107.4",
          "transcript_support_level": 1,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 187,
          "cds_end": null,
          "cds_length": 486,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_130906.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000392283.9"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000286175.12",
          "protein_id": "ENSP00000286175.8",
          "transcript_support_level": 1,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000286175.12"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "n.484G>A",
          "hgvs_p": null,
          "transcript": "ENST00000465823.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000465823.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000409264.6",
          "protein_id": "ENSP00000386893.2",
          "transcript_support_level": 2,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 180,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 543,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409264.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "NM_032472.4",
          "protein_id": "NP_115861.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032472.4"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000409449.5",
          "protein_id": "ENSP00000387012.1",
          "transcript_support_level": 5,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409449.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000899880.1",
          "protein_id": "ENSP00000569939.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899880.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000899882.1",
          "protein_id": "ENSP00000569941.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899882.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000899885.1",
          "protein_id": "ENSP00000569944.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899885.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000917429.1",
          "protein_id": "ENSP00000587488.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917429.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000917431.1",
          "protein_id": "ENSP00000587490.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917431.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000917439.1",
          "protein_id": "ENSP00000587498.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917439.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000917442.1",
          "protein_id": "ENSP00000587501.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917442.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000952533.1",
          "protein_id": "ENSP00000622592.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952533.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser",
          "transcript": "ENST00000952534.1",
          "protein_id": "ENSP00000622593.1",
          "transcript_support_level": null,
          "aa_start": 67,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": 199,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952534.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.187G>A",
          "hgvs_p": "p.Gly63Ser",
          "transcript": "ENST00000899879.1",
          "protein_id": "ENSP00000569938.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 187,
          "cds_end": null,
          "cds_length": 486,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899879.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.187G>A",
          "hgvs_p": "p.Gly63Ser",
          "transcript": "ENST00000899881.1",
          "protein_id": "ENSP00000569940.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 187,
          "cds_end": null,
          "cds_length": 486,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899881.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.187G>A",
          "hgvs_p": "p.Gly63Ser",
          "transcript": "ENST00000899883.1",
          "protein_id": "ENSP00000569942.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 187,
          "cds_end": null,
          "cds_length": 486,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899883.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPIL3",
          "gene_hgnc_id": 9262,
          "hgvs_c": "c.187G>A",
          "hgvs_p": "p.Gly63Ser",
          "transcript": "ENST00000899884.1",
          "protein_id": "ENSP00000569943.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 187,
          "cds_end": null,
          "cds_length": 486,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899884.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
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      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9640008211135864,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.388,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.7942,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.28,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.743,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_032472.4",
          "gene_symbol": "PPIL3",
          "hgnc_id": 9262,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.199G>A",
          "hgvs_p": "p.Gly67Ser"
        },
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000364629.1",
          "gene_symbol": "RNU6-312P",
          "hgnc_id": 47275,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*241G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}