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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-203252041-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=203252041&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 203252041,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001371695.1",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val",
          "transcript": "NM_177538.3",
          "protein_id": "NP_803882.1",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 462,
          "cds_start": 364,
          "cds_end": null,
          "cds_length": 1389,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000356079.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_177538.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val",
          "transcript": "ENST00000356079.9",
          "protein_id": "ENSP00000348380.4",
          "transcript_support_level": 1,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 462,
          "cds_start": 364,
          "cds_end": null,
          "cds_length": 1389,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_177538.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000356079.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "n.364A>G",
          "hgvs_p": null,
          "transcript": "ENST00000449301.5",
          "protein_id": "ENSP00000414831.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000449301.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val",
          "transcript": "ENST00000875109.1",
          "protein_id": "ENSP00000545168.1",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 364,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000875109.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val",
          "transcript": "NM_001371695.1",
          "protein_id": "NP_001358624.1",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 470,
          "cds_start": 364,
          "cds_end": null,
          "cds_length": 1413,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371695.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val",
          "transcript": "ENST00000429815.6",
          "protein_id": "ENSP00000407860.2",
          "transcript_support_level": 2,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 470,
          "cds_start": 364,
          "cds_end": null,
          "cds_length": 1413,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000429815.6"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.262A>G",
          "hgvs_p": "p.Met88Val",
          "transcript": "ENST00000875108.1",
          "protein_id": "ENSP00000545167.1",
          "transcript_support_level": null,
          "aa_start": 88,
          "aa_end": null,
          "aa_length": 428,
          "cds_start": 262,
          "cds_end": null,
          "cds_length": 1287,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000875108.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.58A>G",
          "hgvs_p": "p.Met20Val",
          "transcript": "NM_001371696.1",
          "protein_id": "NP_001358625.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 58,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371696.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.58A>G",
          "hgvs_p": "p.Met20Val",
          "transcript": "NM_001371697.1",
          "protein_id": "NP_001358626.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 58,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371697.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.58A>G",
          "hgvs_p": "p.Met20Val",
          "transcript": "NM_001371698.1",
          "protein_id": "NP_001358627.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 58,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371698.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.58A>G",
          "hgvs_p": "p.Met20Val",
          "transcript": "NM_001371700.1",
          "protein_id": "NP_001358629.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": 58,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371700.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.58A>G",
          "hgvs_p": "p.Met20Val",
          "transcript": "NM_001371701.1",
          "protein_id": "NP_001358630.1",
          "transcript_support_level": null,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": 58,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371701.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val",
          "transcript": "ENST00000443941.1",
          "protein_id": "ENSP00000411341.1",
          "transcript_support_level": 5,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 257,
          "cds_start": 364,
          "cds_end": null,
          "cds_length": 776,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000443941.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val",
          "transcript": "XM_047445157.1",
          "protein_id": "XP_047301113.1",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 233,
          "cds_start": 364,
          "cds_end": null,
          "cds_length": 702,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047445157.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.-242A>G",
          "hgvs_p": null,
          "transcript": "NM_001371702.1",
          "protein_id": "NP_001358631.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371702.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.-242A>G",
          "hgvs_p": null,
          "transcript": "NM_001371703.1",
          "protein_id": "NP_001358632.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371703.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.-242A>G",
          "hgvs_p": null,
          "transcript": "NM_001371705.1",
          "protein_id": "NP_001358634.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371705.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.-242A>G",
          "hgvs_p": null,
          "transcript": "NM_001371706.1",
          "protein_id": "NP_001358635.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001371706.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.72+12907A>G",
          "hgvs_p": null,
          "transcript": "ENST00000956048.1",
          "protein_id": "ENSP00000626107.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956048.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "CYP20A1",
          "gene_hgnc_id": 20576,
          "hgvs_c": "c.-70+38A>G",
          "hgvs_p": null,
          "transcript": "NM_001371699.1",
          "protein_id": "NP_001358628.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 295,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 888,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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      ],
      "gene_symbol": "CYP20A1",
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      "dbsnp": "rs200681711",
      "frequency_reference_population": 0.00025624034,
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      "allele_count_reference_population": 413,
      "gnomad_exomes_af": 0.000240387,
      "gnomad_genomes_af": 0.000408906,
      "gnomad_exomes_ac": 351,
      "gnomad_genomes_ac": 62,
      "gnomad_exomes_homalt": 2,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.01151740550994873,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.16,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0616,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.43,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.444,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -8,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -8,
          "benign_score": 8,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_001371695.1",
          "gene_symbol": "CYP20A1",
          "hgnc_id": 20576,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.364A>G",
          "hgvs_p": "p.Met122Val"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}