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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-216420437-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=216420437&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 216420437,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000357276.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln",
          "transcript": "NM_014140.4",
          "protein_id": "NP_054859.2",
          "transcript_support_level": null,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 954,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 2865,
          "cdna_start": 1261,
          "cdna_end": null,
          "cdna_length": 3201,
          "mane_select": "ENST00000357276.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln",
          "transcript": "ENST00000357276.9",
          "protein_id": "ENSP00000349823.4",
          "transcript_support_level": 2,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 954,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 2865,
          "cdna_start": 1261,
          "cdna_end": null,
          "cdna_length": 3201,
          "mane_select": "NM_014140.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln",
          "transcript": "ENST00000358207.9",
          "protein_id": "ENSP00000350940.5",
          "transcript_support_level": 1,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 954,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 2865,
          "cdna_start": 1152,
          "cdna_end": null,
          "cdna_length": 3056,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.593G>A",
          "hgvs_p": "p.Arg198Gln",
          "transcript": "ENST00000392128.6",
          "protein_id": "ENSP00000375974.2",
          "transcript_support_level": 1,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 796,
          "cds_start": 593,
          "cds_end": null,
          "cds_length": 2391,
          "cdna_start": 593,
          "cdna_end": null,
          "cdna_length": 2431,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln",
          "transcript": "NM_001127207.2",
          "protein_id": "NP_001120679.1",
          "transcript_support_level": null,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 954,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 2865,
          "cdna_start": 1130,
          "cdna_end": null,
          "cdna_length": 3070,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln",
          "transcript": "ENST00000425815.6",
          "protein_id": "ENSP00000394410.2",
          "transcript_support_level": 3,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 954,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 2865,
          "cdna_start": 1253,
          "cdna_end": null,
          "cdna_length": 3176,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln",
          "transcript": "ENST00000430374.6",
          "protein_id": "ENSP00000405077.2",
          "transcript_support_level": 2,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 954,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 2865,
          "cdna_start": 1286,
          "cdna_end": null,
          "cdna_length": 3210,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln",
          "transcript": "ENST00000444508.6",
          "protein_id": "ENSP00000398969.2",
          "transcript_support_level": 2,
          "aa_start": 334,
          "aa_end": null,
          "aa_length": 954,
          "cds_start": 1001,
          "cds_end": null,
          "cds_length": 2865,
          "cdna_start": 1321,
          "cdna_end": null,
          "cdna_length": 3244,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.698G>A",
          "hgvs_p": "p.Arg233Gln",
          "transcript": "ENST00000427645.5",
          "protein_id": "ENSP00000392997.1",
          "transcript_support_level": 3,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 324,
          "cds_start": 698,
          "cds_end": null,
          "cds_length": 977,
          "cdna_start": 698,
          "cdna_end": null,
          "cdna_length": 977,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.161G>A",
          "hgvs_p": "p.Arg54Gln",
          "transcript": "ENST00000412913.1",
          "protein_id": "ENSP00000390248.1",
          "transcript_support_level": 4,
          "aa_start": 54,
          "aa_end": null,
          "aa_length": 140,
          "cds_start": 161,
          "cds_end": null,
          "cds_length": 424,
          "cdna_start": 275,
          "cdna_end": null,
          "cdna_length": 538,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1362G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697898.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3700,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1277G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697900.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3198,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1001G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697901.1",
          "protein_id": "ENSP00000513471.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 3028,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1233G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697902.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3173,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1001G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697903.1",
          "protein_id": "ENSP00000513472.1",
          "transcript_support_level": null,
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          "cdna_start": null,
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          "cdna_length": 3002,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1001G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697904.1",
          "protein_id": "ENSP00000513473.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 2926,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1001G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697905.1",
          "protein_id": "ENSP00000513474.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 2962,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "n.1001G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697907.1",
          "protein_id": "ENSP00000513476.1",
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          "cdna_start": null,
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          "cdna_length": 3054,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.863-3196G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697899.1",
          "protein_id": "ENSP00000513470.1",
          "transcript_support_level": null,
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          "cds_start": -4,
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          "cds_length": 2631,
          "cdna_start": null,
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          "cdna_length": 2977,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SMARCAL1",
          "gene_hgnc_id": 11102,
          "hgvs_c": "c.863-3196G>A",
          "hgvs_p": null,
          "transcript": "ENST00000697906.1",
          "protein_id": "ENSP00000513475.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 876,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 2631,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2826,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SMARCAL1",
      "gene_hgnc_id": 11102,
      "dbsnp": "rs138575228",
      "frequency_reference_population": 0.00006195649,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 100,
      "gnomad_exomes_af": 0.0000478835,
      "gnomad_genomes_af": 0.000197166,
      "gnomad_exomes_ac": 70,
      "gnomad_genomes_ac": 30,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.03695368766784668,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.147,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0672,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.4,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.166,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000357276.9",
          "gene_symbol": "SMARCAL1",
          "hgnc_id": 11102,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1001G>A",
          "hgvs_p": "p.Arg334Gln"
        }
      ],
      "clinvar_disease": "Schimke immuno-osseous dysplasia,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:1",
      "phenotype_combined": "not provided|Schimke immuno-osseous dysplasia",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}