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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-218340131-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=218340131&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 218340131,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000273077.9",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln",
          "transcript": "NM_015488.5",
          "protein_id": "NP_056303.3",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 455,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": 472,
          "cdna_end": null,
          "cdna_length": 2987,
          "mane_select": "ENST00000273077.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln",
          "transcript": "ENST00000273077.9",
          "protein_id": "ENSP00000273077.4",
          "transcript_support_level": 1,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 455,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": 472,
          "cdna_end": null,
          "cdna_length": 2987,
          "mane_select": "NM_015488.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.383G>A",
          "hgvs_p": "p.Arg128Gln",
          "transcript": "ENST00000258362.7",
          "protein_id": "ENSP00000258362.3",
          "transcript_support_level": 1,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 383,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": 478,
          "cdna_end": null,
          "cdna_length": 2991,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.572G>A",
          "hgvs_p": "p.Arg191Gln",
          "transcript": "ENST00000685415.1",
          "protein_id": "ENSP00000510415.1",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 424,
          "cds_start": 572,
          "cds_end": null,
          "cds_length": 1275,
          "cdna_start": 594,
          "cdna_end": null,
          "cdna_length": 3100,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln",
          "transcript": "ENST00000436005.3",
          "protein_id": "ENSP00000414400.3",
          "transcript_support_level": 5,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": 455,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": 477,
          "cdna_end": null,
          "cdna_length": 2974,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln",
          "transcript": "ENST00000689816.1",
          "protein_id": "ENSP00000508450.1",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 455,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": 477,
          "cdna_end": null,
          "cdna_length": 2919,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.383G>A",
          "hgvs_p": "p.Arg128Gln",
          "transcript": "NM_022572.4",
          "protein_id": "NP_072094.1",
          "transcript_support_level": null,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 383,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": 472,
          "cdna_end": null,
          "cdna_length": 2987,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln",
          "transcript": "ENST00000688179.1",
          "protein_id": "ENSP00000508635.1",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 344,
          "cds_start": 455,
          "cds_end": null,
          "cds_length": 1035,
          "cdna_start": 477,
          "cdna_end": null,
          "cdna_length": 2832,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln",
          "transcript": "ENST00000687736.1",
          "protein_id": "ENSP00000509627.1",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 455,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": 455,
          "cdna_end": null,
          "cdna_length": 2753,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln",
          "transcript": "XM_017003771.2",
          "protein_id": "XP_016859260.1",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 356,
          "cds_start": 455,
          "cds_end": null,
          "cds_length": 1071,
          "cdna_start": 472,
          "cdna_end": null,
          "cdna_length": 2900,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.383G>A",
          "hgvs_p": "p.Arg128Gln",
          "transcript": "XM_017003772.2",
          "protein_id": "XP_016859261.1",
          "transcript_support_level": null,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 383,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": 472,
          "cdna_end": null,
          "cdna_length": 2900,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "n.466G>A",
          "hgvs_p": null,
          "transcript": "ENST00000684905.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 3719,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "n.372G>A",
          "hgvs_p": null,
          "transcript": "ENST00000689098.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 3625,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "n.*363G>A",
          "hgvs_p": null,
          "transcript": "ENST00000690891.1",
          "protein_id": "ENSP00000509744.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3106,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "n.*363G>A",
          "hgvs_p": null,
          "transcript": "ENST00000690891.1",
          "protein_id": "ENSP00000509744.1",
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.165-1403G>A",
          "hgvs_p": null,
          "transcript": "ENST00000692295.1",
          "protein_id": "ENSP00000509392.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 265,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 798,
          "cdna_start": null,
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          "cdna_length": 2601,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "c.237-1850G>A",
          "hgvs_p": null,
          "transcript": "ENST00000691220.1",
          "protein_id": "ENSP00000509580.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2580,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CATIP-AS2",
          "gene_hgnc_id": 41079,
          "hgvs_c": "n.146+11029C>T",
          "hgvs_p": null,
          "transcript": "ENST00000411433.2",
          "protein_id": null,
          "transcript_support_level": 3,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "n.240-4677G>A",
          "hgvs_p": null,
          "transcript": "ENST00000691799.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "n.165-4324G>A",
          "hgvs_p": null,
          "transcript": "ENST00000693423.1",
          "protein_id": "ENSP00000508705.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2308,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CATIP-AS2",
          "gene_hgnc_id": 41079,
          "hgvs_c": "n.120+11029C>T",
          "hgvs_p": null,
          "transcript": "NR_125777.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1183,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PNKD",
          "gene_hgnc_id": 9153,
          "hgvs_c": "n.-82G>A",
          "hgvs_p": null,
          "transcript": "ENST00000689693.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3758,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "PNKD",
      "gene_hgnc_id": 9153,
      "dbsnp": "rs73990423",
      "frequency_reference_population": 0.0032053832,
      "hom_count_reference_population": 146,
      "allele_count_reference_population": 5156,
      "gnomad_exomes_af": 0.00177322,
      "gnomad_genomes_af": 0.0169421,
      "gnomad_exomes_ac": 2583,
      "gnomad_genomes_ac": 2573,
      "gnomad_exomes_homalt": 77,
      "gnomad_genomes_homalt": 69,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0025433897972106934,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.187,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0698,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.24,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.238,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000273077.9",
          "gene_symbol": "PNKD",
          "hgnc_id": 9153,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.455G>A",
          "hgvs_p": "p.Arg152Gln"
        },
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000411433.2",
          "gene_symbol": "CATIP-AS2",
          "hgnc_id": 41079,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.146+11029C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "PNKD-related disorder,Paroxysmal nonkinesigenic dyskinesia,Paroxysmal nonkinesigenic dyskinesia 1,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:5",
      "phenotype_combined": "Paroxysmal nonkinesigenic dyskinesia 1|not provided|Paroxysmal nonkinesigenic dyskinesia|PNKD-related disorder|not specified",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}