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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-218661846-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=218661846&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 218661846,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000359273.8",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "NM_001079866.2",
          "protein_id": "NP_001073335.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 658,
          "cdna_end": null,
          "cdna_length": 1427,
          "mane_select": "ENST00000359273.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "ENST00000359273.8",
          "protein_id": "ENSP00000352219.3",
          "transcript_support_level": 1,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 658,
          "cdna_end": null,
          "cdna_length": 1427,
          "mane_select": "NM_001079866.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "ENST00000392109.5",
          "protein_id": "ENSP00000375957.1",
          "transcript_support_level": 1,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 814,
          "cdna_end": null,
          "cdna_length": 1583,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "ENST00000392111.7",
          "protein_id": "ENSP00000375959.2",
          "transcript_support_level": 1,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 867,
          "cdna_end": null,
          "cdna_length": 1636,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "ENST00000412366.5",
          "protein_id": "ENSP00000406494.1",
          "transcript_support_level": 1,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 661,
          "cdna_end": null,
          "cdna_length": 1430,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "NM_001257342.2",
          "protein_id": "NP_001244271.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 788,
          "cdna_end": null,
          "cdna_length": 1557,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "NM_001257343.2",
          "protein_id": "NP_001244272.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 840,
          "cdna_end": null,
          "cdna_length": 1609,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "NM_001257344.2",
          "protein_id": "NP_001244273.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 715,
          "cdna_end": null,
          "cdna_length": 1484,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "NM_001320717.2",
          "protein_id": "NP_001307646.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": 830,
          "cdna_end": null,
          "cdna_length": 1599,
          "mane_select": null,
          "mane_plus": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "NM_001371443.1",
          "protein_id": "NP_001358372.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
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          "cds_start": 548,
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          "cdna_start": 773,
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        {
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          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "NM_001371444.1",
          "protein_id": "NP_001358373.1",
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          "cds_start": 548,
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          "mane_select": null,
          "mane_plus": null,
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        {
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          "gene_symbol": "BCS1L",
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          "hgvs_c": "c.548G>A",
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          "transcript": "NM_001371446.1",
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        {
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          "gene_symbol": "BCS1L",
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          "hgvs_c": "c.548G>A",
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          "transcript": "NM_001371448.1",
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        {
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          "gene_symbol": "BCS1L",
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          "transcript": "NM_001371450.1",
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        {
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          "gene_symbol": "BCS1L",
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          "hgvs_c": "c.548G>A",
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          "transcript": "ENST00000431802.5",
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        {
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          ],
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          "hgvs_c": "c.548G>A",
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          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "n.-5G>A",
          "hgvs_p": null,
          "transcript": "ENST00000460579.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 985,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCS1L",
          "gene_hgnc_id": 1020,
          "hgvs_c": "n.-72G>A",
          "hgvs_p": null,
          "transcript": "ENST00000465706.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 526,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "BCS1L",
      "gene_hgnc_id": 1020,
      "dbsnp": "rs121908577",
      "frequency_reference_population": 0.000005576063,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 9,
      "gnomad_exomes_af": 0.00000547244,
      "gnomad_genomes_af": 0.00000657151,
      "gnomad_exomes_ac": 8,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9709222316741943,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.902,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.6196,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.36,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.151,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 16,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM5,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 16,
          "benign_score": 0,
          "pathogenic_score": 16,
          "criteria": [
            "PM1",
            "PM5",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000359273.8",
          "gene_symbol": "BCS1L",
          "hgnc_id": 1020,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His"
        }
      ],
      "clinvar_disease": "GRACILE syndrome,Leigh syndrome,Mitochondrial complex III deficiency nuclear type 1,Pili torti-deafness syndrome,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:5",
      "phenotype_combined": "Pili torti-deafness syndrome|GRACILE syndrome|Mitochondrial complex III deficiency nuclear type 1|Mitochondrial complex III deficiency nuclear type 1;GRACILE syndrome;Pili torti-deafness syndrome|not provided|Leigh syndrome",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}