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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-218814624-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=218814624&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 218814624,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_000784.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1343G>A",
          "hgvs_p": "p.Arg448His",
          "transcript": "NM_000784.4",
          "protein_id": "NP_000775.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1343,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000258415.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000784.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1343G>A",
          "hgvs_p": "p.Arg448His",
          "transcript": "ENST00000258415.9",
          "protein_id": "ENSP00000258415.4",
          "transcript_support_level": 1,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 531,
          "cds_start": 1343,
          "cds_end": null,
          "cds_length": 1596,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000784.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000258415.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1373G>A",
          "hgvs_p": "p.Arg458His",
          "transcript": "ENST00000901552.1",
          "protein_id": "ENSP00000571611.1",
          "transcript_support_level": null,
          "aa_start": 458,
          "aa_end": null,
          "aa_length": 541,
          "cds_start": 1373,
          "cds_end": null,
          "cds_length": 1626,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901552.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1361G>A",
          "hgvs_p": "p.Arg454His",
          "transcript": "ENST00000901553.1",
          "protein_id": "ENSP00000571612.1",
          "transcript_support_level": null,
          "aa_start": 454,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 1361,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901553.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1337G>A",
          "hgvs_p": "p.Arg446His",
          "transcript": "ENST00000901558.1",
          "protein_id": "ENSP00000571617.1",
          "transcript_support_level": null,
          "aa_start": 446,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 1337,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901558.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1343G>A",
          "hgvs_p": "p.Arg448His",
          "transcript": "ENST00000901557.1",
          "protein_id": "ENSP00000571616.1",
          "transcript_support_level": null,
          "aa_start": 448,
          "aa_end": null,
          "aa_length": 528,
          "cds_start": 1343,
          "cds_end": null,
          "cds_length": 1587,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901557.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1325G>A",
          "hgvs_p": "p.Arg442His",
          "transcript": "ENST00000901561.1",
          "protein_id": "ENSP00000571620.1",
          "transcript_support_level": null,
          "aa_start": 442,
          "aa_end": null,
          "aa_length": 525,
          "cds_start": 1325,
          "cds_end": null,
          "cds_length": 1578,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901561.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1301G>A",
          "hgvs_p": "p.Arg434His",
          "transcript": "ENST00000901554.1",
          "protein_id": "ENSP00000571613.1",
          "transcript_support_level": null,
          "aa_start": 434,
          "aa_end": null,
          "aa_length": 517,
          "cds_start": 1301,
          "cds_end": null,
          "cds_length": 1554,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901554.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1298G>A",
          "hgvs_p": "p.Arg433His",
          "transcript": "ENST00000901555.1",
          "protein_id": "ENSP00000571614.1",
          "transcript_support_level": null,
          "aa_start": 433,
          "aa_end": null,
          "aa_length": 516,
          "cds_start": 1298,
          "cds_end": null,
          "cds_length": 1551,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901555.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1289G>A",
          "hgvs_p": "p.Arg430His",
          "transcript": "ENST00000901562.1",
          "protein_id": "ENSP00000571621.1",
          "transcript_support_level": null,
          "aa_start": 430,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 1289,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901562.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1277G>A",
          "hgvs_p": "p.Arg426His",
          "transcript": "ENST00000901560.1",
          "protein_id": "ENSP00000571619.1",
          "transcript_support_level": null,
          "aa_start": 426,
          "aa_end": null,
          "aa_length": 509,
          "cds_start": 1277,
          "cds_end": null,
          "cds_length": 1530,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901560.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1202G>A",
          "hgvs_p": "p.Arg401His",
          "transcript": "ENST00000901563.1",
          "protein_id": "ENSP00000571622.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 1202,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901563.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1175G>A",
          "hgvs_p": "p.Arg392His",
          "transcript": "ENST00000901564.1",
          "protein_id": "ENSP00000571623.1",
          "transcript_support_level": null,
          "aa_start": 392,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 1175,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901564.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1169G>A",
          "hgvs_p": "p.Arg390His",
          "transcript": "ENST00000901559.1",
          "protein_id": "ENSP00000571618.1",
          "transcript_support_level": null,
          "aa_start": 390,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 1169,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901559.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "c.1145G>A",
          "hgvs_p": "p.Arg382His",
          "transcript": "ENST00000901556.1",
          "protein_id": "ENSP00000571615.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 1145,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000901556.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP27A1",
          "gene_hgnc_id": 2605,
          "hgvs_c": "n.2055G>A",
          "hgvs_p": null,
          "transcript": "ENST00000494263.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000494263.5"
        }
      ],
      "gene_symbol": "CYP27A1",
      "gene_hgnc_id": 2605,
      "dbsnp": "rs370868184",
      "frequency_reference_population": 0.000056998837,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 92,
      "gnomad_exomes_af": 0.0000471996,
      "gnomad_genomes_af": 0.000151125,
      "gnomad_exomes_ac": 69,
      "gnomad_genomes_ac": 23,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9188601970672607,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.9,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2295,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": 0.45,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 6.852,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_000784.4",
          "gene_symbol": "CYP27A1",
          "hgnc_id": 2605,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1343G>A",
          "hgvs_p": "p.Arg448His"
        }
      ],
      "clinvar_disease": "Cholestanol storage disease,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "phenotype_combined": "not provided|Cholestanol storage disease",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}