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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-227330777-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=227330777&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 227330777,
      "ref": "C",
      "alt": "T",
      "effect": "stop_gained",
      "transcript": "ENST00000304593.14",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "NM_001277062.2",
          "protein_id": "NP_001263991.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 876,
          "cdna_start": 441,
          "cdna_end": null,
          "cdna_length": 1921,
          "mane_select": "ENST00000304593.14",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000304593.14",
          "protein_id": "ENSP00000304898.10",
          "transcript_support_level": 2,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 876,
          "cdna_start": 441,
          "cdna_end": null,
          "cdna_length": 1921,
          "mane_select": "NM_001277062.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000337110.11",
          "protein_id": "ENSP00000338412.7",
          "transcript_support_level": 1,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 243,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 732,
          "cdna_start": 424,
          "cdna_end": null,
          "cdna_length": 1760,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Gln64*",
          "transcript": "NM_001277061.2",
          "protein_id": "NP_001263990.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": 619,
          "cdna_end": null,
          "cdna_length": 2174,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Gln64*",
          "transcript": "NM_020194.5",
          "protein_id": "NP_064579.3",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": 456,
          "cdna_end": null,
          "cdna_length": 2014,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Gln64*",
          "transcript": "ENST00000353339.8",
          "protein_id": "ENSP00000302037.4",
          "transcript_support_level": 5,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": 619,
          "cdna_end": null,
          "cdna_length": 2174,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000707109.1",
          "protein_id": "ENSP00000516738.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 316,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 951,
          "cdna_start": 426,
          "cdna_end": null,
          "cdna_length": 1955,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000707111.1",
          "protein_id": "ENSP00000516740.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": 269,
          "cdna_end": null,
          "cdna_length": 1628,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Gln64*",
          "transcript": "ENST00000707110.1",
          "protein_id": "ENSP00000516739.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 300,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 903,
          "cdna_start": 555,
          "cdna_end": null,
          "cdna_length": 1329,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000456345.2",
          "protein_id": "ENSP00000415313.2",
          "transcript_support_level": 2,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 271,
          "cds_start": 112,
          "cds_end": null,
          "cds_length": 816,
          "cdna_start": 371,
          "cdna_end": null,
          "cdna_length": 1713,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Gln64*",
          "transcript": "ENST00000409616.5",
          "protein_id": "ENSP00000386641.2",
          "transcript_support_level": 5,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 264,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 795,
          "cdna_start": 608,
          "cdna_end": null,
          "cdna_length": 1247,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "NM_001277068.1",
          "protein_id": "NP_001263997.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 252,
          "cds_start": 112,
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          "cds_length": 759,
          "cdna_start": 264,
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          "cdna_length": 1630,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "NM_001277063.2",
          "protein_id": "NP_001263992.1",
          "transcript_support_level": null,
          "aa_start": 38,
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          "aa_length": 243,
          "cds_start": 112,
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          "cdna_start": 441,
          "cdna_end": null,
          "cdna_length": 1777,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
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          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "NM_001277064.2",
          "protein_id": "NP_001263993.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 238,
          "cds_start": 112,
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          "cds_length": 717,
          "cdna_start": 441,
          "cdna_end": null,
          "cdna_length": 1762,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
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          "protein_coding": true,
          "strand": true,
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          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
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          "protein_id": "ENSP00000304134.9",
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 3,
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          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "NM_001277065.2",
          "protein_id": "NP_001263994.1",
          "transcript_support_level": null,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 112,
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          "cdna_start": 441,
          "cdna_end": null,
          "cdna_length": 1702,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 2,
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          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "NM_001277066.2",
          "protein_id": "NP_001263995.1",
          "transcript_support_level": null,
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          "cdna_start": 329,
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          "cdna_length": 1590,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
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          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000354503.10",
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        },
        {
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          ],
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          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000409565.5",
          "protein_id": "ENSP00000386964.1",
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
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          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*",
          "transcript": "ENST00000452930.5",
          "protein_id": "ENSP00000415996.1",
          "transcript_support_level": 4,
          "aa_start": 38,
          "aa_end": null,
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          "cds_start": 112,
          "cds_end": null,
          "cds_length": 414,
          "cdna_start": 434,
          "cdna_end": null,
          "cdna_length": 736,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFF",
          "gene_hgnc_id": 24858,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Gln64*",
          "transcript": "ENST00000418961.5",
          "protein_id": "ENSP00000407547.2",
          "transcript_support_level": 5,
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        {
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          "gene_symbol": "MFF",
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          "transcript": "ENST00000423098.5",
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        }
      ],
      "gene_symbol": "MFF",
      "gene_hgnc_id": 24858,
      "dbsnp": "rs397514615",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.28999999165534973,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.29,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 1.316,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5",
      "acmg_by_gene": [
        {
          "score": 11,
          "benign_score": 0,
          "pathogenic_score": 11,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000304593.14",
          "gene_symbol": "MFF",
          "hgnc_id": 24858,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.112C>T",
          "hgvs_p": "p.Gln38*"
        }
      ],
      "clinvar_disease": "Encephalopathy due to defective mitochondrial and peroxisomal fission 2,Global developmental delay,Mitochondrial encephalomyopathy",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Encephalopathy due to defective mitochondrial and peroxisomal fission 2|Global developmental delay;Mitochondrial encephalomyopathy",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}