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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-24079596-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=24079596&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 24079596,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_004881.5",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.664T>A",
          "hgvs_p": "p.Trp222Arg",
          "transcript": "NM_004881.5",
          "protein_id": "NP_004872.2",
          "transcript_support_level": null,
          "aa_start": 222,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 664,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": 1172,
          "cdna_end": null,
          "cdna_length": 1653,
          "mane_select": "ENST00000238721.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004881.5"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.664T>A",
          "hgvs_p": "p.Trp222Arg",
          "transcript": "ENST00000238721.9",
          "protein_id": "ENSP00000238721.4",
          "transcript_support_level": 1,
          "aa_start": 222,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 664,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": 1172,
          "cdna_end": null,
          "cdna_length": 1653,
          "mane_select": "NM_004881.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000238721.9"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.664T>A",
          "hgvs_p": "p.Trp222Arg",
          "transcript": "ENST00000335934.8",
          "protein_id": "ENSP00000337834.4",
          "transcript_support_level": 1,
          "aa_start": 222,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 664,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": 1154,
          "cdna_end": null,
          "cdna_length": 1635,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000335934.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.619+1223T>A",
          "hgvs_p": null,
          "transcript": "ENST00000407482.5",
          "protein_id": "ENSP00000384414.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 248,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 747,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 747,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000407482.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM228B",
          "gene_hgnc_id": 24736,
          "hgvs_c": "c.-289-1280A>T",
          "hgvs_p": null,
          "transcript": "ENST00000613899.4",
          "protein_id": "ENSP00000479742.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 187,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 564,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1238,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000613899.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM228B",
          "gene_hgnc_id": 24736,
          "hgvs_c": "n.91-1283A>T",
          "hgvs_p": null,
          "transcript": "ENST00000469867.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2495,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000469867.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.664T>A",
          "hgvs_p": "p.Trp222Arg",
          "transcript": "NM_147184.4",
          "protein_id": "NP_671713.1",
          "transcript_support_level": null,
          "aa_start": 222,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 664,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": 773,
          "cdna_end": null,
          "cdna_length": 1254,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_147184.4"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.640T>A",
          "hgvs_p": "p.Trp214Arg",
          "transcript": "ENST00000861137.1",
          "protein_id": "ENSP00000531196.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 324,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 975,
          "cdna_start": 1362,
          "cdna_end": null,
          "cdna_length": 1840,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000861137.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.625T>A",
          "hgvs_p": "p.Trp209Arg",
          "transcript": "ENST00000861138.1",
          "protein_id": "ENSP00000531197.1",
          "transcript_support_level": null,
          "aa_start": 209,
          "aa_end": null,
          "aa_length": 319,
          "cds_start": 625,
          "cds_end": null,
          "cds_length": 960,
          "cdna_start": 1132,
          "cdna_end": null,
          "cdna_length": 1613,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000861138.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.451T>A",
          "hgvs_p": "p.Trp151Arg",
          "transcript": "ENST00000923540.1",
          "protein_id": "ENSP00000593599.1",
          "transcript_support_level": null,
          "aa_start": 151,
          "aa_end": null,
          "aa_length": 261,
          "cds_start": 451,
          "cds_end": null,
          "cds_length": 786,
          "cdna_start": 541,
          "cdna_end": null,
          "cdna_length": 1019,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923540.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.397T>A",
          "hgvs_p": "p.Trp133Arg",
          "transcript": "XM_024453249.2",
          "protein_id": "XP_024309017.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 243,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 732,
          "cdna_start": 410,
          "cdna_end": null,
          "cdna_length": 891,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024453249.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.619+1223T>A",
          "hgvs_p": null,
          "transcript": "NM_001206802.2",
          "protein_id": "NP_001193731.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 248,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 747,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 953,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001206802.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM228B",
          "gene_hgnc_id": 24736,
          "hgvs_c": "c.-289-1280A>T",
          "hgvs_p": null,
          "transcript": "NM_001291328.2",
          "protein_id": "NP_001278257.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 187,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 564,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1287,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001291328.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "c.619+1223T>A",
          "hgvs_p": null,
          "transcript": "XM_006712150.3",
          "protein_id": "XP_006712213.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 248,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 747,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1057,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006712150.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TP53I3",
          "gene_hgnc_id": 19373,
          "hgvs_c": "n.733T>A",
          "hgvs_p": null,
          "transcript": "ENST00000417886.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 827,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000417886.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM228B",
          "gene_hgnc_id": 24736,
          "hgvs_c": "n.277+415A>T",
          "hgvs_p": null,
          "transcript": "ENST00000460686.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 357,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000460686.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM228B",
          "gene_hgnc_id": 24736,
          "hgvs_c": "n.113-1280A>T",
          "hgvs_p": null,
          "transcript": "ENST00000461972.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2616,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000461972.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM228B",
          "gene_hgnc_id": 24736,
          "hgvs_c": "n.43-1280A>T",
          "hgvs_p": null,
          "transcript": "ENST00000486967.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 581,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000486967.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FAM228B",
          "gene_hgnc_id": 24736,
          "hgvs_c": "n.138-1283A>T",
          "hgvs_p": null,
          "transcript": "NR_111929.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 793,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_111929.2"
        }
      ],
      "gene_symbol": "TP53I3",
      "gene_hgnc_id": 19373,
      "dbsnp": "rs1043776561",
      "frequency_reference_population": 0.000013010685,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 21,
      "gnomad_exomes_af": 0.0000123128,
      "gnomad_genomes_af": 0.0000197156,
      "gnomad_exomes_ac": 18,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8214480876922607,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.07999999821186066,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.468,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9254,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.06,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 5.269,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.08,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004881.5",
          "gene_symbol": "TP53I3",
          "hgnc_id": 19373,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.664T>A",
          "hgvs_p": "p.Trp222Arg"
        },
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001291328.2",
          "gene_symbol": "FAM228B",
          "hgnc_id": 24736,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-289-1280A>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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