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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-241767734-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=241767734&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 241767734,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_152783.5",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1331T>C",
          "hgvs_p": "p.Val444Ala",
          "transcript": "NM_152783.5",
          "protein_id": "NP_689996.4",
          "transcript_support_level": null,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 521,
          "cds_start": 1331,
          "cds_end": null,
          "cds_length": 1566,
          "cdna_start": 1489,
          "cdna_end": null,
          "cdna_length": 2566,
          "mane_select": "ENST00000321264.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_152783.5"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1331T>C",
          "hgvs_p": "p.Val444Ala",
          "transcript": "ENST00000321264.9",
          "protein_id": "ENSP00000315351.4",
          "transcript_support_level": 1,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 521,
          "cds_start": 1331,
          "cds_end": null,
          "cds_length": 1566,
          "cdna_start": 1489,
          "cdna_end": null,
          "cdna_length": 2566,
          "mane_select": "NM_152783.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000321264.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "n.*2567T>C",
          "hgvs_p": null,
          "transcript": "ENST00000436747.5",
          "protein_id": "ENSP00000400212.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4549,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000436747.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "n.1221T>C",
          "hgvs_p": null,
          "transcript": "ENST00000468064.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2298,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000468064.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "n.812T>C",
          "hgvs_p": null,
          "transcript": "ENST00000470343.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1026,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000470343.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "n.530T>C",
          "hgvs_p": null,
          "transcript": "ENST00000473126.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1612,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000473126.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "n.1155T>C",
          "hgvs_p": null,
          "transcript": "ENST00000486953.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2232,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000486953.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "n.*2567T>C",
          "hgvs_p": null,
          "transcript": "ENST00000436747.5",
          "protein_id": "ENSP00000400212.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4549,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000436747.5"
        },
        {
          "aa_ref": "*",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_lost"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1243T>C",
          "hgvs_p": "p.Ter415Argext*?",
          "transcript": "XM_011511750.4",
          "protein_id": "XP_011510052.1",
          "transcript_support_level": null,
          "aa_start": 415,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 1243,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": 1401,
          "cdna_end": null,
          "cdna_length": 2478,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011511750.4"
        },
        {
          "aa_ref": "*",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "stop_lost"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1165T>C",
          "hgvs_p": "p.Ter389Argext*?",
          "transcript": "XM_047445718.1",
          "protein_id": "XP_047301674.1",
          "transcript_support_level": null,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": 1165,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": 1323,
          "cdna_end": null,
          "cdna_length": 2400,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047445718.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1556T>C",
          "hgvs_p": "p.Val519Ala",
          "transcript": "ENST00000951632.1",
          "protein_id": "ENSP00000621691.1",
          "transcript_support_level": null,
          "aa_start": 519,
          "aa_end": null,
          "aa_length": 596,
          "cds_start": 1556,
          "cds_end": null,
          "cds_length": 1791,
          "cdna_start": 1714,
          "cdna_end": null,
          "cdna_length": 2795,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951632.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1517T>C",
          "hgvs_p": "p.Val506Ala",
          "transcript": "ENST00000951631.1",
          "protein_id": "ENSP00000621690.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": 1682,
          "cdna_end": null,
          "cdna_length": 2759,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951631.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1451T>C",
          "hgvs_p": "p.Val484Ala",
          "transcript": "ENST00000951636.1",
          "protein_id": "ENSP00000621695.1",
          "transcript_support_level": null,
          "aa_start": 484,
          "aa_end": null,
          "aa_length": 561,
          "cds_start": 1451,
          "cds_end": null,
          "cds_length": 1686,
          "cdna_start": 1588,
          "cdna_end": null,
          "cdna_length": 2665,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951636.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1412T>C",
          "hgvs_p": "p.Val471Ala",
          "transcript": "ENST00000951634.1",
          "protein_id": "ENSP00000621693.1",
          "transcript_support_level": null,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 548,
          "cds_start": 1412,
          "cds_end": null,
          "cds_length": 1647,
          "cdna_start": 1569,
          "cdna_end": null,
          "cdna_length": 2644,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951634.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1409T>C",
          "hgvs_p": "p.Val470Ala",
          "transcript": "ENST00000910513.1",
          "protein_id": "ENSP00000580572.1",
          "transcript_support_level": null,
          "aa_start": 470,
          "aa_end": null,
          "aa_length": 547,
          "cds_start": 1409,
          "cds_end": null,
          "cds_length": 1644,
          "cdna_start": 1595,
          "cdna_end": null,
          "cdna_length": 2677,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910513.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1409T>C",
          "hgvs_p": "p.Val470Ala",
          "transcript": "ENST00000910524.1",
          "protein_id": "ENSP00000580583.1",
          "transcript_support_level": null,
          "aa_start": 470,
          "aa_end": null,
          "aa_length": 547,
          "cds_start": 1409,
          "cds_end": null,
          "cds_length": 1644,
          "cdna_start": 1730,
          "cdna_end": null,
          "cdna_length": 2804,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910524.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1406T>C",
          "hgvs_p": "p.Val469Ala",
          "transcript": "ENST00000910522.1",
          "protein_id": "ENSP00000580581.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 546,
          "cds_start": 1406,
          "cds_end": null,
          "cds_length": 1641,
          "cdna_start": 1565,
          "cdna_end": null,
          "cdna_length": 2642,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910522.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1373T>C",
          "hgvs_p": "p.Val458Ala",
          "transcript": "ENST00000910517.1",
          "protein_id": "ENSP00000580576.1",
          "transcript_support_level": null,
          "aa_start": 458,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 1373,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": 1552,
          "cdna_end": null,
          "cdna_length": 2629,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910517.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1349T>C",
          "hgvs_p": "p.Val450Ala",
          "transcript": "ENST00000910519.1",
          "protein_id": "ENSP00000580578.1",
          "transcript_support_level": null,
          "aa_start": 450,
          "aa_end": null,
          "aa_length": 527,
          "cds_start": 1349,
          "cds_end": null,
          "cds_length": 1584,
          "cdna_start": 1519,
          "cdna_end": null,
          "cdna_length": 2596,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910519.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "D2HGDH",
          "gene_hgnc_id": 28358,
          "hgvs_c": "c.1331T>C",
          "hgvs_p": "p.Val444Ala",
          "transcript": "ENST00000910514.1",
          "protein_id": "ENSP00000580573.1",
          "transcript_support_level": null,
          "aa_start": 444,
          "aa_end": null,
          "aa_length": 521,
          "cds_start": 1331,
          "cds_end": null,
          "cds_length": 1566,
          "cdna_start": 1513,
          "cdna_end": null,
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      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.895,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.606,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.2,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.238,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_152783.5",
          "gene_symbol": "D2HGDH",
          "hgnc_id": 28358,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1331T>C",
          "hgvs_p": "p.Val444Ala"
        }
      ],
      "clinvar_disease": "D-2-hydroxyglutaric aciduria 1",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "D-2-hydroxyglutaric aciduria 1",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}
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