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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-26279244-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=26279244&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 26279244,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_000183.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "NM_000183.3",
          "protein_id": "NP_000174.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000317799.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000183.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000317799.10",
          "protein_id": "ENSP00000325136.5",
          "transcript_support_level": 1,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000183.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000317799.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.824G>A",
          "hgvs_p": "p.Arg275His",
          "transcript": "ENST00000942431.1",
          "protein_id": "ENSP00000612490.1",
          "transcript_support_level": null,
          "aa_start": 275,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 824,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942431.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.770G>A",
          "hgvs_p": "p.Arg257His",
          "transcript": "ENST00000942426.1",
          "protein_id": "ENSP00000612485.1",
          "transcript_support_level": null,
          "aa_start": 257,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 770,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942426.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.767G>A",
          "hgvs_p": "p.Arg256His",
          "transcript": "ENST00000859601.1",
          "protein_id": "ENSP00000529660.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859601.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.752G>A",
          "hgvs_p": "p.Arg251His",
          "transcript": "ENST00000942427.1",
          "protein_id": "ENSP00000612486.1",
          "transcript_support_level": null,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 478,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 1437,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942427.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000859582.1",
          "protein_id": "ENSP00000529641.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859582.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000859593.1",
          "protein_id": "ENSP00000529652.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859593.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000859599.1",
          "protein_id": "ENSP00000529658.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859599.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942424.1",
          "protein_id": "ENSP00000612483.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942424.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942425.1",
          "protein_id": "ENSP00000612484.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942425.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942434.1",
          "protein_id": "ENSP00000612493.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942434.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942437.1",
          "protein_id": "ENSP00000612496.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942437.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942440.1",
          "protein_id": "ENSP00000612499.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942440.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942441.1",
          "protein_id": "ENSP00000612500.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942441.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942445.1",
          "protein_id": "ENSP00000612504.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942445.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000859584.1",
          "protein_id": "ENSP00000529643.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000859584.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000859587.1",
          "protein_id": "ENSP00000529646.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 740,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859587.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His",
          "transcript": "ENST00000942429.1",
          "protein_id": "ENSP00000612488.1",
          "transcript_support_level": null,
          "aa_start": 247,
          "aa_end": null,
          "aa_length": 470,
          "cds_start": 740,
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          "cds_length": 1413,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942429.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HADHB",
          "gene_hgnc_id": 4803,
          "hgvs_c": "c.722G>A",
          "hgvs_p": "p.Arg241His",
          "transcript": "ENST00000859600.1",
          "protein_id": "ENSP00000529659.1",
          "transcript_support_level": null,
          "aa_start": 241,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": 722,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859600.1"
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          "biotype": "pseudogene",
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      ],
      "gene_symbol": "HADHB",
      "gene_hgnc_id": 4803,
      "dbsnp": "rs121913133",
      "frequency_reference_population": 0.000010952078,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 16,
      "gnomad_exomes_af": 0.0000109521,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 16,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9697001576423645,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.816,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.3159,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.41,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.525,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 17,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PM5,PP2,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 17,
          "benign_score": 0,
          "pathogenic_score": 17,
          "criteria": [
            "PM2",
            "PM5",
            "PP2",
            "PP3_Strong",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "NM_000183.3",
          "gene_symbol": "HADHB",
          "hgnc_id": 4803,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.740G>A",
          "hgvs_p": "p.Arg247His"
        }
      ],
      "clinvar_disease": "Mitochondrial trifunctional protein deficiency,Mitochondrial trifunctional protein deficiency 1,Mitochondrial trifunctional protein deficiency 2,not provided",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:1 LP:3",
      "phenotype_combined": "not provided|Mitochondrial trifunctional protein deficiency 2|Mitochondrial trifunctional protein deficiency|Mitochondrial trifunctional protein deficiency 1",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}