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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-27364515-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=27364515&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 27364515,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001318965.2",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1457A>G",
          "hgvs_p": "p.Asn486Ser",
          "transcript": "NM_001034116.2",
          "protein_id": "NP_001029288.1",
          "transcript_support_level": null,
          "aa_start": 486,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 1457,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000347454.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001034116.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1457A>G",
          "hgvs_p": "p.Asn486Ser",
          "transcript": "ENST00000347454.9",
          "protein_id": "ENSP00000233552.6",
          "transcript_support_level": 1,
          "aa_start": 486,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 1457,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001034116.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000347454.9"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1517A>G",
          "hgvs_p": "p.Asn506Ser",
          "transcript": "ENST00000451130.6",
          "protein_id": "ENSP00000394869.2",
          "transcript_support_level": 1,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000451130.6"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1454A>G",
          "hgvs_p": "p.Asn485Ser",
          "transcript": "ENST00000445933.6",
          "protein_id": "ENSP00000394397.2",
          "transcript_support_level": 1,
          "aa_start": 485,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1454,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000445933.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "n.*723A>G",
          "hgvs_p": null,
          "transcript": "ENST00000405940.6",
          "protein_id": "ENSP00000384375.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000405940.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "n.*723A>G",
          "hgvs_p": null,
          "transcript": "ENST00000405940.6",
          "protein_id": "ENSP00000384375.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000405940.6"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1538A>G",
          "hgvs_p": "p.Asn513Ser",
          "transcript": "ENST00000945158.1",
          "protein_id": "ENSP00000615217.1",
          "transcript_support_level": null,
          "aa_start": 513,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 1538,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945158.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1520A>G",
          "hgvs_p": "p.Asn507Ser",
          "transcript": "NM_001318965.2",
          "protein_id": "NP_001305894.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 1520,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318965.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1520A>G",
          "hgvs_p": "p.Asn507Ser",
          "transcript": "ENST00000493344.6",
          "protein_id": "ENSP00000429323.1",
          "transcript_support_level": 5,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 1520,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000493344.6"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1517A>G",
          "hgvs_p": "p.Asn506Ser",
          "transcript": "NM_172195.4",
          "protein_id": "NP_751945.2",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_172195.4"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1493A>G",
          "hgvs_p": "p.Asn498Ser",
          "transcript": "ENST00000920939.1",
          "protein_id": "ENSP00000590998.1",
          "transcript_support_level": null,
          "aa_start": 498,
          "aa_end": null,
          "aa_length": 535,
          "cds_start": 1493,
          "cds_end": null,
          "cds_length": 1608,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920939.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1478A>G",
          "hgvs_p": "p.Asn493Ser",
          "transcript": "ENST00000945157.1",
          "protein_id": "ENSP00000615216.1",
          "transcript_support_level": null,
          "aa_start": 493,
          "aa_end": null,
          "aa_length": 530,
          "cds_start": 1478,
          "cds_end": null,
          "cds_length": 1593,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945157.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1475A>G",
          "hgvs_p": "p.Asn492Ser",
          "transcript": "ENST00000945155.1",
          "protein_id": "ENSP00000615214.1",
          "transcript_support_level": null,
          "aa_start": 492,
          "aa_end": null,
          "aa_length": 529,
          "cds_start": 1475,
          "cds_end": null,
          "cds_length": 1590,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945155.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1454A>G",
          "hgvs_p": "p.Asn485Ser",
          "transcript": "NM_015636.4",
          "protein_id": "NP_056451.3",
          "transcript_support_level": null,
          "aa_start": 485,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1454,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_015636.4"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1454A>G",
          "hgvs_p": "p.Asn485Ser",
          "transcript": "ENST00000920928.1",
          "protein_id": "ENSP00000590987.1",
          "transcript_support_level": null,
          "aa_start": 485,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1454,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920928.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1454A>G",
          "hgvs_p": "p.Asn485Ser",
          "transcript": "ENST00000920929.1",
          "protein_id": "ENSP00000590988.1",
          "transcript_support_level": null,
          "aa_start": 485,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1454,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920929.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1451A>G",
          "hgvs_p": "p.Asn484Ser",
          "transcript": "ENST00000920931.1",
          "protein_id": "ENSP00000590990.1",
          "transcript_support_level": null,
          "aa_start": 484,
          "aa_end": null,
          "aa_length": 521,
          "cds_start": 1451,
          "cds_end": null,
          "cds_length": 1566,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920931.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1451A>G",
          "hgvs_p": "p.Asn484Ser",
          "transcript": "ENST00000945153.1",
          "protein_id": "ENSP00000615212.1",
          "transcript_support_level": null,
          "aa_start": 484,
          "aa_end": null,
          "aa_length": 521,
          "cds_start": 1451,
          "cds_end": null,
          "cds_length": 1566,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945153.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1412A>G",
          "hgvs_p": "p.Asn471Ser",
          "transcript": "NM_001318966.2",
          "protein_id": "NP_001305895.1",
          "transcript_support_level": null,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 1412,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318966.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.1409A>G",
          "hgvs_p": "p.Asn470Ser",
          "transcript": "ENST00000945152.1",
          "protein_id": "ENSP00000615211.1",
          "transcript_support_level": null,
          "aa_start": 470,
          "aa_end": null,
          "aa_length": 507,
          "cds_start": 1409,
          "cds_end": null,
          "cds_length": 1524,
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        {
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      ],
      "gene_symbol": "EIF2B4",
      "gene_hgnc_id": 3260,
      "dbsnp": "rs1167111337",
      "frequency_reference_population": 0.0000055760283,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 9,
      "gnomad_exomes_af": 0.00000410426,
      "gnomad_genomes_af": 0.0000197163,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9436227083206177,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.874,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2449,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 6.104,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
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      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM1",
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001318965.2",
          "gene_symbol": "EIF2B4",
          "hgnc_id": 3260,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1520A>G",
          "hgvs_p": "p.Asn507Ser"
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        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000412749.2",
          "gene_symbol": "GTF3C2-AS2",
          "hgnc_id": 55699,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.238-2909T>C",
          "hgvs_p": null
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      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}