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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-27368047-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=27368047&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 27368047,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000347454.9",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.683C>T",
          "hgvs_p": "p.Ala228Val",
          "transcript": "NM_001034116.2",
          "protein_id": "NP_001029288.1",
          "transcript_support_level": null,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 683,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": 707,
          "cdna_end": null,
          "cdna_length": 1644,
          "mane_select": "ENST00000347454.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.683C>T",
          "hgvs_p": "p.Ala228Val",
          "transcript": "ENST00000347454.9",
          "protein_id": "ENSP00000233552.6",
          "transcript_support_level": 1,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 523,
          "cds_start": 683,
          "cds_end": null,
          "cds_length": 1572,
          "cdna_start": 707,
          "cdna_end": null,
          "cdna_length": 1644,
          "mane_select": "NM_001034116.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.743C>T",
          "hgvs_p": "p.Ala248Val",
          "transcript": "ENST00000451130.6",
          "protein_id": "ENSP00000394869.2",
          "transcript_support_level": 1,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": 743,
          "cdna_end": null,
          "cdna_length": 1633,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.680C>T",
          "hgvs_p": "p.Ala227Val",
          "transcript": "ENST00000445933.6",
          "protein_id": "ENSP00000394397.2",
          "transcript_support_level": 1,
          "aa_start": 227,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 680,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": 700,
          "cdna_end": null,
          "cdna_length": 1608,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "n.657C>T",
          "hgvs_p": null,
          "transcript": "ENST00000405940.6",
          "protein_id": "ENSP00000384375.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1570,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.746C>T",
          "hgvs_p": "p.Ala249Val",
          "transcript": "NM_001318965.2",
          "protein_id": "NP_001305894.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": 758,
          "cdna_end": null,
          "cdna_length": 1695,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.746C>T",
          "hgvs_p": "p.Ala249Val",
          "transcript": "ENST00000493344.6",
          "protein_id": "ENSP00000429323.1",
          "transcript_support_level": 5,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": 1059,
          "cdna_end": null,
          "cdna_length": 1949,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.743C>T",
          "hgvs_p": "p.Ala248Val",
          "transcript": "NM_172195.4",
          "protein_id": "NP_751945.2",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": 755,
          "cdna_end": null,
          "cdna_length": 1692,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.680C>T",
          "hgvs_p": "p.Ala227Val",
          "transcript": "NM_015636.4",
          "protein_id": "NP_056451.3",
          "transcript_support_level": null,
          "aa_start": 227,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 680,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": 704,
          "cdna_end": null,
          "cdna_length": 1641,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.638C>T",
          "hgvs_p": "p.Ala213Val",
          "transcript": "NM_001318966.2",
          "protein_id": "NP_001305895.1",
          "transcript_support_level": null,
          "aa_start": 213,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": 638,
          "cds_end": null,
          "cds_length": 1527,
          "cdna_start": 885,
          "cdna_end": null,
          "cdna_length": 1822,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.590C>T",
          "hgvs_p": "p.Ala197Val",
          "transcript": "NM_001318967.2",
          "protein_id": "NP_001305896.1",
          "transcript_support_level": null,
          "aa_start": 197,
          "aa_end": null,
          "aa_length": 492,
          "cds_start": 590,
          "cds_end": null,
          "cds_length": 1479,
          "cdna_start": 700,
          "cdna_end": null,
          "cdna_length": 1637,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.98C>T",
          "hgvs_p": "p.Ala33Val",
          "transcript": "NM_001318968.2",
          "protein_id": "NP_001305897.1",
          "transcript_support_level": null,
          "aa_start": 33,
          "aa_end": null,
          "aa_length": 328,
          "cds_start": 98,
          "cds_end": null,
          "cds_length": 987,
          "cdna_start": 688,
          "cdna_end": null,
          "cdna_length": 1625,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "c.65C>T",
          "hgvs_p": "p.Ala22Val",
          "transcript": "NM_001318969.2",
          "protein_id": "NP_001305898.1",
          "transcript_support_level": null,
          "aa_start": 22,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 65,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": 681,
          "cdna_end": null,
          "cdna_length": 1618,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "n.259C>T",
          "hgvs_p": null,
          "transcript": "ENST00000417567.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 761,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EIF2B4",
          "gene_hgnc_id": 3260,
          "hgvs_c": "n.1804C>T",
          "hgvs_p": null,
          "transcript": "ENST00000475582.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4293,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GTF3C2-AS2",
          "gene_hgnc_id": 55699,
          "hgvs_c": "n.*166G>A",
          "hgvs_p": null,
          "transcript": "ENST00000412749.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 695,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GTF3C2-AS2",
          "gene_hgnc_id": 55699,
          "hgvs_c": "n.*167G>A",
          "hgvs_p": null,
          "transcript": "ENST00000847761.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 759,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GTF3C2-AS2",
          "gene_hgnc_id": 55699,
          "hgvs_c": "n.*167G>A",
          "hgvs_p": null,
          "transcript": "NR_183825.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2202,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GTF3C2-AS2",
          "gene_hgnc_id": 55699,
          "hgvs_c": "n.*167G>A",
          "hgvs_p": null,
          "transcript": "NR_183826.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1125,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GTF3C2-AS2",
          "gene_hgnc_id": 55699,
          "hgvs_c": "n.*167G>A",
          "hgvs_p": null,
          "transcript": "NR_183827.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1220,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GTF3C2-AS2",
          "gene_hgnc_id": 55699,
          "hgvs_c": "n.*167G>A",
          "hgvs_p": null,
          "transcript": "NR_183831.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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        {
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          "cdna_length": 705,
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      ],
      "gene_symbol": "EIF2B4",
      "gene_hgnc_id": 3260,
      "dbsnp": "rs113994027",
      "frequency_reference_population": 0.0000013881178,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000138812,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9773944616317749,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.922,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.3602,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.5,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.052,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 7,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 7,
          "benign_score": 0,
          "pathogenic_score": 7,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000347454.9",
          "gene_symbol": "EIF2B4",
          "hgnc_id": 3260,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.683C>T",
          "hgvs_p": "p.Ala228Val"
        },
        {
          "score": 7,
          "benign_score": 0,
          "pathogenic_score": 7,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000412749.2",
          "gene_symbol": "GTF3C2-AS2",
          "hgnc_id": 55699,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*166G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Leukoencephalopathy with vanishing white matter 4",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Leukoencephalopathy with vanishing white matter 4",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}