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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-28783934-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=28783934&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 28783934,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_002709.3",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "NM_002709.3",
          "protein_id": "NP_002700.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000395366.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002709.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "ENST00000395366.3",
          "protein_id": "ENSP00000378769.2",
          "transcript_support_level": 1,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002709.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395366.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "ENST00000296122.10",
          "protein_id": "ENSP00000296122.6",
          "transcript_support_level": 1,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000296122.10"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.671A>C",
          "hgvs_p": "p.Glu224Ala",
          "transcript": "ENST00000703174.1",
          "protein_id": "ENSP00000515220.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000703174.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.671A>C",
          "hgvs_p": "p.Glu224Ala",
          "transcript": "ENST00000941106.1",
          "protein_id": "ENSP00000611165.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 368,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 1107,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941106.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.575A>C",
          "hgvs_p": "p.Glu192Ala",
          "transcript": "ENST00000868413.1",
          "protein_id": "ENSP00000538472.1",
          "transcript_support_level": null,
          "aa_start": 192,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 575,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868413.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.575A>C",
          "hgvs_p": "p.Glu192Ala",
          "transcript": "ENST00000941109.1",
          "protein_id": "ENSP00000611168.1",
          "transcript_support_level": null,
          "aa_start": 192,
          "aa_end": null,
          "aa_length": 336,
          "cds_start": 575,
          "cds_end": null,
          "cds_length": 1011,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941109.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "NM_206876.2",
          "protein_id": "NP_996759.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_206876.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "ENST00000420282.6",
          "protein_id": "ENSP00000398839.2",
          "transcript_support_level": 4,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000420282.6"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "ENST00000441461.6",
          "protein_id": "ENSP00000414918.2",
          "transcript_support_level": 2,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000441461.6"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "ENST00000703173.1",
          "protein_id": "ENSP00000515219.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000703173.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.545A>C",
          "hgvs_p": "p.Glu182Ala",
          "transcript": "ENST00000941105.1",
          "protein_id": "ENSP00000611164.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 545,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941105.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.545A>C",
          "hgvs_p": "p.Glu182Ala",
          "transcript": "ENST00000941108.1",
          "protein_id": "ENSP00000611167.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 545,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941108.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.536A>C",
          "hgvs_p": "p.Glu179Ala",
          "transcript": "ENST00000868411.1",
          "protein_id": "ENSP00000538470.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 323,
          "cds_start": 536,
          "cds_end": null,
          "cds_length": 972,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868411.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.515A>C",
          "hgvs_p": "p.Glu172Ala",
          "transcript": "ENST00000703176.1",
          "protein_id": "ENSP00000515221.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 316,
          "cds_start": 515,
          "cds_end": null,
          "cds_length": 951,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000703176.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.548A>C",
          "hgvs_p": "p.Glu183Ala",
          "transcript": "ENST00000941113.1",
          "protein_id": "ENSP00000611172.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 316,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 951,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941113.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.482A>C",
          "hgvs_p": "p.Glu161Ala",
          "transcript": "ENST00000941110.1",
          "protein_id": "ENSP00000611169.1",
          "transcript_support_level": null,
          "aa_start": 161,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 482,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941110.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.464A>C",
          "hgvs_p": "p.Glu155Ala",
          "transcript": "ENST00000455580.6",
          "protein_id": "ENSP00000390715.2",
          "transcript_support_level": 3,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 464,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000455580.6"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.464A>C",
          "hgvs_p": "p.Glu155Ala",
          "transcript": "ENST00000703172.1",
          "protein_id": "ENSP00000515218.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 464,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000703172.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP1CB",
          "gene_hgnc_id": 9282,
          "hgvs_c": "c.464A>C",
          "hgvs_p": "p.Glu155Ala",
          "transcript": "ENST00000868412.1",
          "protein_id": "ENSP00000538471.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 464,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000868412.1"
        },
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      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.611345112323761,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.283,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.8069,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.15,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 9.294,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
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      "dbscsnv_ada_score": null,
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      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PS2,PP2,PM2_Supporting,PS4_Supporting,BP4",
      "acmg_by_gene": [
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            "PP2",
            "PM2_Supporting",
            "PS4_Supporting",
            "BP4"
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          "verdict": "Pathogenic",
          "transcript": "NM_002709.3",
          "gene_symbol": "PPP1CB",
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          "effects": [
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        {
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          "inheritance_mode": "AR",
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      ],
      "clinvar_disease": "Cardiovascular phenotype,Noonan syndrome-like disorder with loose anagen hair,Noonan syndrome-like disorder with loose anagen hair 2,RASopathy,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "reviewed by expert panel",
      "clinvar_submissions_summary": "P:6",
      "phenotype_combined": "Noonan syndrome-like disorder with loose anagen hair 2|not provided|Cardiovascular phenotype|Noonan syndrome-like disorder with loose anagen hair|RASopathy",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}