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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-32126964-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=32126964&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 32126964,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_014946.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Ile",
          "transcript": "NM_014946.4",
          "protein_id": "NP_055761.2",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 616,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 1851,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000315285.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014946.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Ile",
          "transcript": "ENST00000315285.9",
          "protein_id": "ENSP00000320885.3",
          "transcript_support_level": 1,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 616,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 1851,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_014946.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000315285.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1112G>T",
          "hgvs_p": "p.Arg371Ile",
          "transcript": "ENST00000621856.2",
          "protein_id": "ENSP00000482496.2",
          "transcript_support_level": 1,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000621856.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1220G>T",
          "hgvs_p": "p.Arg407Ile",
          "transcript": "ENST00000713716.1",
          "protein_id": "ENSP00000519019.1",
          "transcript_support_level": null,
          "aa_start": 407,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1220,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000713716.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1112G>T",
          "hgvs_p": "p.Arg371Ile",
          "transcript": "NM_001363823.2",
          "protein_id": "NP_001350752.1",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1848,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363823.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Ile",
          "transcript": "ENST00000933132.1",
          "protein_id": "ENSP00000603191.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 614,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 1845,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933132.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1019G>T",
          "hgvs_p": "p.Arg340Ile",
          "transcript": "NM_199436.2",
          "protein_id": "NP_955468.1",
          "transcript_support_level": null,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 584,
          "cds_start": 1019,
          "cds_end": null,
          "cds_length": 1755,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_199436.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1019G>T",
          "hgvs_p": "p.Arg340Ile",
          "transcript": "ENST00000646571.1",
          "protein_id": "ENSP00000495015.1",
          "transcript_support_level": null,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 584,
          "cds_start": 1019,
          "cds_end": null,
          "cds_length": 1755,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000646571.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1016G>T",
          "hgvs_p": "p.Arg339Ile",
          "transcript": "NM_001363875.2",
          "protein_id": "NP_001350804.1",
          "transcript_support_level": null,
          "aa_start": 339,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 1016,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363875.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1016G>T",
          "hgvs_p": "p.Arg339Ile",
          "transcript": "ENST00000642455.1",
          "protein_id": "ENSP00000493827.1",
          "transcript_support_level": null,
          "aa_start": 339,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 1016,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642455.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1112G>T",
          "hgvs_p": "p.Arg371Ile",
          "transcript": "ENST00000933133.1",
          "protein_id": "ENSP00000603192.1",
          "transcript_support_level": null,
          "aa_start": 371,
          "aa_end": null,
          "aa_length": 574,
          "cds_start": 1112,
          "cds_end": null,
          "cds_length": 1725,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933133.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1115G>T",
          "hgvs_p": "p.Arg372Ile",
          "transcript": "ENST00000644408.2",
          "protein_id": "ENSP00000493748.2",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 571,
          "cds_start": 1115,
          "cds_end": null,
          "cds_length": 1716,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000644408.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.857G>T",
          "hgvs_p": "p.Arg286Ile",
          "transcript": "ENST00000642999.1",
          "protein_id": "ENSP00000496589.1",
          "transcript_support_level": null,
          "aa_start": 286,
          "aa_end": null,
          "aa_length": 530,
          "cds_start": 857,
          "cds_end": null,
          "cds_length": 1593,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642999.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1019G>T",
          "hgvs_p": "p.Arg340Ile",
          "transcript": "NM_001377959.1",
          "protein_id": "NP_001364888.1",
          "transcript_support_level": null,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 519,
          "cds_start": 1019,
          "cds_end": null,
          "cds_length": 1560,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001377959.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
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          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1019G>T",
          "hgvs_p": "p.Arg340Ile",
          "transcript": "ENST00000642751.2",
          "protein_id": "ENSP00000495478.2",
          "transcript_support_level": null,
          "aa_start": 340,
          "aa_end": null,
          "aa_length": 519,
          "cds_start": 1019,
          "cds_end": null,
          "cds_length": 1560,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642751.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.761G>T",
          "hgvs_p": "p.Arg254Ile",
          "transcript": "ENST00000644954.1",
          "protein_id": "ENSP00000494312.1",
          "transcript_support_level": null,
          "aa_start": 254,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 761,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000644954.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.944G>T",
          "hgvs_p": "p.Arg315Ile",
          "transcript": "ENST00000645671.2",
          "protein_id": "ENSP00000494601.2",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 494,
          "cds_start": 944,
          "cds_end": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
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          ],
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          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.461G>T",
          "hgvs_p": "p.Arg154Ile",
          "transcript": "ENST00000645730.1",
          "protein_id": "ENSP00000496072.1",
          "transcript_support_level": null,
          "aa_start": 154,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 461,
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          "cds_length": 1028,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000645730.1"
        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 16,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1099-1444G>T",
          "hgvs_p": null,
          "transcript": "ENST00000647133.2",
          "protein_id": "ENSP00000493742.2",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 591,
          "cds_start": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000647133.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "SPAST",
          "gene_hgnc_id": 11233,
          "hgvs_c": "c.1099-9599G>T",
          "hgvs_p": null,
          "transcript": "ENST00000642281.2",
          "protein_id": "ENSP00000496211.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642281.2"
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      "computational_source_selected": "MetaRNN",
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      "splice_source_selected": "max_spliceai",
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      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.02,
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      "acmg_by_gene": [
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            "PP3_Moderate"
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          "verdict": "Likely_pathogenic",
          "transcript": "NM_014946.4",
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      "clinvar_disease": "Hereditary spastic paraplegia 4",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Hereditary spastic paraplegia 4",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
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  ],
  "message": null
}