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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-33522043-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=33522043&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 33522043,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_170672.3",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "NM_001139488.2",
          "protein_id": "NP_001132960.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000403687.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001139488.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000403687.8",
          "protein_id": "ENSP00000384192.3",
          "transcript_support_level": 1,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001139488.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000403687.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000402538.8",
          "protein_id": "ENSP00000385886.3",
          "transcript_support_level": 1,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000402538.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000407811.5",
          "protein_id": "ENSP00000383917.1",
          "transcript_support_level": 1,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 689,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2070,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000407811.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000850562.1",
          "protein_id": "ENSP00000520853.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000850562.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000850563.1",
          "protein_id": "ENSP00000520854.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000850563.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000967278.1",
          "protein_id": "ENSP00000637337.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 700,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2103,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967278.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "NM_001349975.2",
          "protein_id": "NP_001336904.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349975.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "NM_001349976.2",
          "protein_id": "NP_001336905.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349976.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "NM_001349977.2",
          "protein_id": "NP_001336906.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001349977.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "NM_170672.3",
          "protein_id": "NP_733772.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_170672.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000884676.1",
          "protein_id": "ENSP00000554735.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884676.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000884677.1",
          "protein_id": "ENSP00000554736.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884677.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000884679.1",
          "protein_id": "ENSP00000554738.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884679.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000884682.1",
          "protein_id": "ENSP00000554741.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884682.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000884683.1",
          "protein_id": "ENSP00000554742.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884683.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000884685.1",
          "protein_id": "ENSP00000554744.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000884685.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000884686.1",
          "protein_id": "ENSP00000554745.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000884686.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000967275.1",
          "protein_id": "ENSP00000637334.1",
          "transcript_support_level": null,
          "aa_start": 153,
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          "aa_length": 690,
          "cds_start": 457,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967275.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RASGRP3",
          "gene_hgnc_id": 14545,
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser",
          "transcript": "ENST00000967276.1",
          "protein_id": "ENSP00000637335.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 690,
          "cds_start": 457,
          "cds_end": null,
          "cds_length": 2073,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967276.1"
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      ],
      "gene_symbol": "RASGRP3",
      "gene_hgnc_id": 14545,
      "dbsnp": "rs750271899",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8223418593406677,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.317,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.437,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.905,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_170672.3",
          "gene_symbol": "RASGRP3",
          "hgnc_id": 14545,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.457C>T",
          "hgvs_p": "p.Pro153Ser"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}