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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-38074853-GC-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=38074853&ref=GC&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 38074853,
      "ref": "GC",
      "alt": "G",
      "effect": "frameshift_variant",
      "transcript": "ENST00000610745.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1",
          "gene_hgnc_id": 2597,
          "hgvs_c": "c.535delG",
          "hgvs_p": "p.Ala179fs",
          "transcript": "NM_000104.4",
          "protein_id": "NP_000095.2",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": 908,
          "cdna_end": null,
          "cdna_length": 5218,
          "mane_select": "ENST00000610745.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1",
          "gene_hgnc_id": 2597,
          "hgvs_c": "c.535delG",
          "hgvs_p": "p.Ala179fs",
          "transcript": "ENST00000610745.5",
          "protein_id": "ENSP00000478561.1",
          "transcript_support_level": 1,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": 908,
          "cdna_end": null,
          "cdna_length": 5218,
          "mane_select": "NM_000104.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1",
          "gene_hgnc_id": 2597,
          "hgvs_c": "c.535delG",
          "hgvs_p": "p.Ala179fs",
          "transcript": "ENST00000490576.2",
          "protein_id": "ENSP00000478839.2",
          "transcript_support_level": 4,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": 711,
          "cdna_end": null,
          "cdna_length": 5021,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1",
          "gene_hgnc_id": 2597,
          "hgvs_c": "c.535delG",
          "hgvs_p": "p.Ala179fs",
          "transcript": "ENST00000614273.1",
          "protein_id": "ENSP00000483678.1",
          "transcript_support_level": 5,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": 837,
          "cdna_end": null,
          "cdna_length": 2174,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1",
          "gene_hgnc_id": 2597,
          "hgvs_c": "c.535delG",
          "hgvs_p": "p.Ala179fs",
          "transcript": "ENST00000714520.1",
          "protein_id": "ENSP00000519767.1",
          "transcript_support_level": null,
          "aa_start": 179,
          "aa_end": null,
          "aa_length": 543,
          "cds_start": 535,
          "cds_end": null,
          "cds_length": 1632,
          "cdna_start": 938,
          "cdna_end": null,
          "cdna_length": 5248,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1",
          "gene_hgnc_id": 2597,
          "hgvs_c": "c.-70-3544delG",
          "hgvs_p": null,
          "transcript": "ENST00000494864.1",
          "protein_id": "ENSP00000479876.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 172,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 519,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1479,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1",
          "gene_hgnc_id": 2597,
          "hgvs_c": "n.376-446delG",
          "hgvs_p": null,
          "transcript": "ENST00000613082.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 573,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.116+477delC",
          "hgvs_p": null,
          "transcript": "ENST00000628232.2",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 635,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.165+428delC",
          "hgvs_p": null,
          "transcript": "ENST00000629719.2",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 606,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.164+428delC",
          "hgvs_p": null,
          "transcript": "ENST00000767219.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 536,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.23+667delC",
          "hgvs_p": null,
          "transcript": "ENST00000767220.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 641,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.90+428delC",
          "hgvs_p": null,
          "transcript": "ENST00000767221.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 703,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.-26delC",
          "hgvs_p": null,
          "transcript": "ENST00000627992.4",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.-19delC",
          "hgvs_p": null,
          "transcript": "ENST00000628135.3",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2188,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.-26delC",
          "hgvs_p": null,
          "transcript": "ENST00000767222.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 683,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.-37delC",
          "hgvs_p": null,
          "transcript": "ENST00000767223.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 898,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.-47delC",
          "hgvs_p": null,
          "transcript": "ENST00000767224.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 641,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CYP1B1-AS1",
          "gene_hgnc_id": 28543,
          "hgvs_c": "n.-75delC",
          "hgvs_p": null,
          "transcript": "ENST00000767225.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 637,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "CYP1B1",
      "gene_hgnc_id": 2597,
      "dbsnp": "rs771076928",
      "frequency_reference_population": 0.00005118074,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 80,
      "gnomad_exomes_af": 0.0000503285,
      "gnomad_genomes_af": 0.0000590722,
      "gnomad_exomes_ac": 71,
      "gnomad_genomes_ac": 9,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": -0.027,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 16,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 16,
          "benign_score": 0,
          "pathogenic_score": 16,
          "criteria": [
            "PVS1",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000610745.5",
          "gene_symbol": "CYP1B1",
          "hgnc_id": 2597,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.535delG",
          "hgvs_p": "p.Ala179fs"
        },
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PP5_Very_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000767219.1",
          "gene_symbol": "CYP1B1-AS1",
          "hgnc_id": 28543,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.164+428delC",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " B, primary infantile,Anterior segment dysgenesis 6,CYP1B1-related disorder,Congenital glaucoma,Glaucoma 3,Glaucoma 3A,Primary congenital glaucoma,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:9",
      "phenotype_combined": "not provided|Congenital glaucoma|Anterior segment dysgenesis 6|Glaucoma 3A|CYP1B1-related disorder|Anterior segment dysgenesis 6;Glaucoma 3A;Glaucoma 3, primary infantile, B|Primary congenital glaucoma",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}