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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-54982628-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=54982628&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 54982628,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_020532.5",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.3247A>G",
          "hgvs_p": "p.Ile1083Val",
          "transcript": "NM_020532.5",
          "protein_id": "NP_065393.1",
          "transcript_support_level": null,
          "aa_start": 1083,
          "aa_end": null,
          "aa_length": 1192,
          "cds_start": 3247,
          "cds_end": null,
          "cds_length": 3579,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000337526.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020532.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.3247A>G",
          "hgvs_p": "p.Ile1083Val",
          "transcript": "ENST00000337526.11",
          "protein_id": "ENSP00000337838.6",
          "transcript_support_level": 1,
          "aa_start": 1083,
          "aa_end": null,
          "aa_length": 1192,
          "cds_start": 3247,
          "cds_end": null,
          "cds_length": 3579,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_020532.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000337526.11"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "ENST00000357376.7",
          "protein_id": "ENSP00000349944.3",
          "transcript_support_level": 1,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000357376.7"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "ENST00000394611.6",
          "protein_id": "ENSP00000378109.2",
          "transcript_support_level": 1,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394611.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "ENST00000404909.5",
          "protein_id": "ENSP00000385650.1",
          "transcript_support_level": 1,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000404909.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "ENST00000405240.5",
          "protein_id": "ENSP00000384471.1",
          "transcript_support_level": 1,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000405240.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.847A>G",
          "hgvs_p": "p.Ile283Val",
          "transcript": "ENST00000357732.8",
          "protein_id": "ENSP00000350365.4",
          "transcript_support_level": 1,
          "aa_start": 283,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 847,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000357732.8"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.790A>G",
          "hgvs_p": "p.Ile264Val",
          "transcript": "ENST00000317610.11",
          "protein_id": "ENSP00000322147.7",
          "transcript_support_level": 1,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 373,
          "cds_start": 790,
          "cds_end": null,
          "cds_length": 1122,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000317610.11"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.268A>G",
          "hgvs_p": "p.Ile90Val",
          "transcript": "ENST00000394609.6",
          "protein_id": "ENSP00000378107.2",
          "transcript_support_level": 1,
          "aa_start": 90,
          "aa_end": null,
          "aa_length": 199,
          "cds_start": 268,
          "cds_end": null,
          "cds_length": 600,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000394609.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "NM_001321859.2",
          "protein_id": "NP_001308788.1",
          "transcript_support_level": null,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001321859.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "NM_001321860.1",
          "protein_id": "NP_001308789.1",
          "transcript_support_level": null,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001321860.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "NM_001321861.2",
          "protein_id": "NP_001308790.1",
          "transcript_support_level": null,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 986,
          "cds_start": 2629,
          "cds_end": null,
          "cds_length": 2961,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001321861.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "NM_001321862.2",
          "protein_id": "NP_001308791.1",
          "transcript_support_level": null,
          "aa_start": 877,
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          "cds_start": 2629,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001321862.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "NM_001321863.2",
          "protein_id": "NP_001308792.1",
          "transcript_support_level": null,
          "aa_start": 877,
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          "aa_length": 986,
          "cds_start": 2629,
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          "feature": "NM_001321863.2"
        },
        {
          "aa_ref": "I",
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          "exon_rank": 6,
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          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "NM_001321904.2",
          "protein_id": "NP_001308833.1",
          "transcript_support_level": null,
          "aa_start": 877,
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          "aa_length": 986,
          "cds_start": 2629,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001321904.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2629A>G",
          "hgvs_p": "p.Ile877Val",
          "transcript": "NM_207521.2",
          "protein_id": "NP_997404.1",
          "transcript_support_level": null,
          "aa_start": 877,
          "aa_end": null,
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          "biotype": "protein_coding",
          "feature": "NM_207521.2"
        },
        {
          "aa_ref": "I",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.2410A>G",
          "hgvs_p": "p.Ile804Val",
          "transcript": "ENST00000938587.1",
          "protein_id": "ENSP00000608646.1",
          "transcript_support_level": null,
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          "cds_start": 2410,
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        {
          "aa_ref": "I",
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          ],
          "exon_rank": 5,
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.991A>G",
          "hgvs_p": "p.Ile331Val",
          "transcript": "ENST00000951906.1",
          "protein_id": "ENSP00000621965.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 991,
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          "cds_length": 1323,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000951906.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.874A>G",
          "hgvs_p": "p.Ile292Val",
          "transcript": "ENST00000852508.1",
          "protein_id": "ENSP00000522567.1",
          "transcript_support_level": null,
          "aa_start": 292,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 874,
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          "cds_length": 1206,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852508.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "c.847A>G",
          "hgvs_p": "p.Ile283Val",
          "transcript": "NM_207520.2",
          "protein_id": "NP_997403.1",
          "transcript_support_level": null,
          "aa_start": 283,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 847,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": null,
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        {
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          "exon_count": 6,
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          "gene_symbol": "RTN4",
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          "hgvs_c": "c.316A>G",
          "hgvs_p": "p.Ile106Val",
          "transcript": "ENST00000438462.1",
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          "cds_start": 316,
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        {
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          "gene_symbol": "RTN4",
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          "hgvs_p": "p.Ile90Val",
          "transcript": "NM_007008.3",
          "protein_id": "NP_008939.1",
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          "cds_start": 268,
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          "feature": "NM_007008.3"
        },
        {
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          "strand": false,
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RTN4",
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          "hgvs_c": "c.169A>G",
          "hgvs_p": "p.Ile57Val",
          "transcript": "ENST00000486085.5",
          "protein_id": "ENSP00000489133.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000486085.5"
        },
        {
          "aa_ref": null,
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          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
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          "exon_count": 7,
          "intron_rank": null,
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          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "n.368A>G",
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          "transcript": "NR_135829.2",
          "protein_id": null,
          "transcript_support_level": null,
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          "aa_length": null,
          "cds_start": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "pseudogene",
          "feature": "NR_135829.2"
        },
        {
          "aa_ref": null,
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          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
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          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTN4",
          "gene_hgnc_id": 14085,
          "hgvs_c": "n.312A>G",
          "hgvs_p": null,
          "transcript": "NR_135830.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_135830.2"
        }
      ],
      "gene_symbol": "RTN4",
      "gene_hgnc_id": 14085,
      "dbsnp": "rs200090073",
      "frequency_reference_population": 0.000008694572,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 14,
      "gnomad_exomes_af": 0.00000754447,
      "gnomad_genomes_af": 0.0000197138,
      "gnomad_exomes_ac": 11,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.07793989777565002,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.044,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0748,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.51,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.392,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_020532.5",
          "gene_symbol": "RTN4",
          "hgnc_id": 14085,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.3247A>G",
          "hgvs_p": "p.Ile1083Val"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}