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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-71682612-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=71682612&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 71682612,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000410020.8",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6256A>G",
          "hgvs_p": "p.Ile2086Val",
          "transcript": "NM_001130987.2",
          "protein_id": "NP_001124459.1",
          "transcript_support_level": null,
          "aa_start": 2086,
          "aa_end": null,
          "aa_length": 2119,
          "cds_start": 6256,
          "cds_end": null,
          "cds_length": 6360,
          "cdna_start": 6400,
          "cdna_end": null,
          "cdna_length": 6775,
          "mane_select": "ENST00000410020.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6256A>G",
          "hgvs_p": "p.Ile2086Val",
          "transcript": "ENST00000410020.8",
          "protein_id": "ENSP00000386881.3",
          "transcript_support_level": 1,
          "aa_start": 2086,
          "aa_end": null,
          "aa_length": 2119,
          "cds_start": 6256,
          "cds_end": null,
          "cds_length": 6360,
          "cdna_start": 6400,
          "cdna_end": null,
          "cdna_length": 6775,
          "mane_select": "NM_001130987.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 54,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6139A>G",
          "hgvs_p": "p.Ile2047Val",
          "transcript": "NM_003494.4",
          "protein_id": "NP_003485.1",
          "transcript_support_level": null,
          "aa_start": 2047,
          "aa_end": null,
          "aa_length": 2080,
          "cds_start": 6139,
          "cds_end": null,
          "cds_length": 6243,
          "cdna_start": 6577,
          "cdna_end": null,
          "cdna_length": 6952,
          "mane_select": null,
          "mane_plus": "ENST00000258104.8",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 54,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6139A>G",
          "hgvs_p": "p.Ile2047Val",
          "transcript": "ENST00000258104.8",
          "protein_id": "ENSP00000258104.3",
          "transcript_support_level": 1,
          "aa_start": 2047,
          "aa_end": null,
          "aa_length": 2080,
          "cds_start": 6139,
          "cds_end": null,
          "cds_length": 6243,
          "cdna_start": 6577,
          "cdna_end": null,
          "cdna_length": 6952,
          "mane_select": null,
          "mane_plus": "NM_003494.4",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6253A>G",
          "hgvs_p": "p.Ile2085Val",
          "transcript": "ENST00000409582.7",
          "protein_id": "ENSP00000386547.3",
          "transcript_support_level": 1,
          "aa_start": 2085,
          "aa_end": null,
          "aa_length": 2118,
          "cds_start": 6253,
          "cds_end": null,
          "cds_length": 6357,
          "cdna_start": 6530,
          "cdna_end": null,
          "cdna_length": 6790,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6235A>G",
          "hgvs_p": "p.Ile2079Val",
          "transcript": "ENST00000409651.5",
          "protein_id": "ENSP00000386683.1",
          "transcript_support_level": 1,
          "aa_start": 2079,
          "aa_end": null,
          "aa_length": 2112,
          "cds_start": 6235,
          "cds_end": null,
          "cds_length": 6339,
          "cdna_start": 6376,
          "cdna_end": null,
          "cdna_length": 6636,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6232A>G",
          "hgvs_p": "p.Ile2078Val",
          "transcript": "ENST00000413539.6",
          "protein_id": "ENSP00000407046.2",
          "transcript_support_level": 1,
          "aa_start": 2078,
          "aa_end": null,
          "aa_length": 2111,
          "cds_start": 6232,
          "cds_end": null,
          "cds_length": 6336,
          "cdna_start": 6509,
          "cdna_end": null,
          "cdna_length": 6769,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6205A>G",
          "hgvs_p": "p.Ile2069Val",
          "transcript": "ENST00000409366.5",
          "protein_id": "ENSP00000386512.1",
          "transcript_support_level": 1,
          "aa_start": 2069,
          "aa_end": null,
          "aa_length": 2102,
          "cds_start": 6205,
          "cds_end": null,
          "cds_length": 6309,
          "cdna_start": 6346,
          "cdna_end": null,
          "cdna_length": 6606,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6202A>G",
          "hgvs_p": "p.Ile2068Val",
          "transcript": "ENST00000429174.6",
          "protein_id": "ENSP00000398305.2",
          "transcript_support_level": 1,
          "aa_start": 2068,
          "aa_end": null,
          "aa_length": 2101,
          "cds_start": 6202,
          "cds_end": null,
          "cds_length": 6306,
          "cdna_start": 6479,
          "cdna_end": null,
          "cdna_length": 6739,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 54,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6193A>G",
          "hgvs_p": "p.Ile2065Val",
          "transcript": "ENST00000410041.1",
          "protein_id": "ENSP00000386617.1",
          "transcript_support_level": 1,
          "aa_start": 2065,
          "aa_end": null,
          "aa_length": 2098,
          "cds_start": 6193,
          "cds_end": null,
          "cds_length": 6297,
          "cdna_start": 6334,
          "cdna_end": null,
          "cdna_length": 6594,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 54,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6190A>G",
          "hgvs_p": "p.Ile2064Val",
          "transcript": "ENST00000409762.5",
          "protein_id": "ENSP00000387137.1",
          "transcript_support_level": 1,
          "aa_start": 2064,
          "aa_end": null,
          "aa_length": 2097,
          "cds_start": 6190,
          "cds_end": null,
          "cds_length": 6294,
          "cdna_start": 6467,
          "cdna_end": null,
          "cdna_length": 6727,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 54,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6163A>G",
          "hgvs_p": "p.Ile2055Val",
          "transcript": "ENST00000409744.5",
          "protein_id": "ENSP00000386285.1",
          "transcript_support_level": 1,
          "aa_start": 2055,
          "aa_end": null,
          "aa_length": 2088,
          "cds_start": 6163,
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          "cdna_start": 6304,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          "exon_rank": 54,
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          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6142A>G",
          "hgvs_p": "p.Ile2048Val",
          "transcript": "ENST00000394120.6",
          "protein_id": "ENSP00000377678.2",
          "transcript_support_level": 1,
          "aa_start": 2048,
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          "cds_start": 6142,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6253A>G",
          "hgvs_p": "p.Ile2085Val",
          "transcript": "NM_001130981.2",
          "protein_id": "NP_001124453.1",
          "transcript_support_level": null,
          "aa_start": 2085,
          "aa_end": null,
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          "cds_start": 6253,
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          "feature": null
        },
        {
          "aa_ref": "I",
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          "intron_rank": null,
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          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6235A>G",
          "hgvs_p": "p.Ile2079Val",
          "transcript": "NM_001130982.2",
          "protein_id": "NP_001124454.1",
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6232A>G",
          "hgvs_p": "p.Ile2078Val",
          "transcript": "NM_001130979.2",
          "protein_id": "NP_001124451.1",
          "transcript_support_level": null,
          "aa_start": 2078,
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          "feature": null
        },
        {
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 55,
          "exon_rank_end": null,
          "exon_count": 56,
          "intron_rank": null,
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          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6205A>G",
          "hgvs_p": "p.Ile2069Val",
          "transcript": "NM_001130983.2",
          "protein_id": "NP_001124455.1",
          "transcript_support_level": null,
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        {
          "aa_ref": "I",
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          "exon_rank": 55,
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          "exon_count": 56,
          "intron_rank": null,
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          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6202A>G",
          "hgvs_p": "p.Ile2068Val",
          "transcript": "NM_001130978.2",
          "protein_id": "NP_001124450.1",
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        },
        {
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          "strand": true,
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          ],
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          "gene_symbol": "DYSF",
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          "hgvs_c": "c.6193A>G",
          "hgvs_p": "p.Ile2065Val",
          "transcript": "NM_001130985.2",
          "protein_id": "NP_001124457.1",
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 54,
          "exon_rank_end": null,
          "exon_count": 55,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DYSF",
          "gene_hgnc_id": 3097,
          "hgvs_c": "c.6190A>G",
          "hgvs_p": "p.Ile2064Val",
          "transcript": "NM_001130980.2",
          "protein_id": "NP_001124452.1",
          "transcript_support_level": null,
          "aa_start": 2064,
          "aa_end": null,
          "aa_length": 2097,
          "cds_start": 6190,
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          "cds_length": 6294,
          "cdna_start": 6628,
          "cdna_end": null,
          "cdna_length": 7003,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
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      "dbsnp": "rs150834671",
      "frequency_reference_population": 0.00037738046,
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      "allele_count_reference_population": 609,
      "gnomad_exomes_af": 0.00039271,
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      "gnomad_genomes_ac": 35,
      "gnomad_exomes_homalt": 1,
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      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.03323709964752197,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.287,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0824,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.21,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.672,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
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          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
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          "verdict": "Likely_benign",
          "transcript": "ENST00000410020.8",
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          "hgvs_p": "p.Ile2086Val"
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      "clinvar_disease": " recessive,Autosomal recessive limb-girdle muscular dystrophy type 2B,Limb-girdle muscular dystrophy,Miyoshi muscular dystrophy 1,Miyoshi myopathy,Neuromuscular disease caused by qualitative or quantitative defects of dysferlin,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:8 LB:1",
      "phenotype_combined": "Miyoshi myopathy|Limb-girdle muscular dystrophy, recessive|Autosomal recessive limb-girdle muscular dystrophy type 2B|not provided|Miyoshi muscular dystrophy 1|Neuromuscular disease caused by qualitative or quantitative defects of dysferlin",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}