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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-74527986-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=74527986&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 74527986,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_181575.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "NM_181575.5",
          "protein_id": "NP_853553.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 410,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1233,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000377526.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_181575.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000377526.4",
          "protein_id": "ENSP00000366748.3",
          "transcript_support_level": 1,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 410,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1233,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_181575.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000377526.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "n.692G>A",
          "hgvs_p": null,
          "transcript": "ENST00000425118.5",
          "protein_id": "ENSP00000403430.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000425118.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "n.956G>A",
          "hgvs_p": null,
          "transcript": "ENST00000463900.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000463900.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890035.1",
          "protein_id": "ENSP00000560094.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 422,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1269,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890035.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000954392.1",
          "protein_id": "ENSP00000624451.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 421,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1266,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954392.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890029.1",
          "protein_id": "ENSP00000560088.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 420,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1263,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890029.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890022.1",
          "protein_id": "ENSP00000560081.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 411,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1236,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890022.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890033.1",
          "protein_id": "ENSP00000560092.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 409,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1230,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890033.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.689G>A",
          "hgvs_p": "p.Arg230His",
          "transcript": "ENST00000890037.1",
          "protein_id": "ENSP00000560096.1",
          "transcript_support_level": null,
          "aa_start": 230,
          "aa_end": null,
          "aa_length": 409,
          "cds_start": 689,
          "cds_end": null,
          "cds_length": 1230,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890037.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890024.1",
          "protein_id": "ENSP00000560083.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 407,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1224,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890024.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.665G>A",
          "hgvs_p": "p.Arg222His",
          "transcript": "ENST00000954391.1",
          "protein_id": "ENSP00000624450.1",
          "transcript_support_level": null,
          "aa_start": 222,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 665,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954391.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890027.1",
          "protein_id": "ENSP00000560086.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890027.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890026.1",
          "protein_id": "ENSP00000560085.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 386,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1161,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890026.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.614G>A",
          "hgvs_p": "p.Arg205His",
          "transcript": "ENST00000954390.1",
          "protein_id": "ENSP00000624449.1",
          "transcript_support_level": null,
          "aa_start": 205,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 614,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000954390.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.692G>A",
          "hgvs_p": "p.Arg231His",
          "transcript": "ENST00000890023.1",
          "protein_id": "ENSP00000560082.1",
          "transcript_support_level": null,
          "aa_start": 231,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": 692,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890023.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.554G>A",
          "hgvs_p": "p.Arg185His",
          "transcript": "ENST00000936888.1",
          "protein_id": "ENSP00000606947.1",
          "transcript_support_level": null,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936888.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.554G>A",
          "hgvs_p": "p.Arg185His",
          "transcript": "ENST00000890021.1",
          "protein_id": "ENSP00000560080.1",
          "transcript_support_level": null,
          "aa_start": 185,
          "aa_end": null,
          "aa_length": 364,
          "cds_start": 554,
          "cds_end": null,
          "cds_length": 1095,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890021.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.548G>A",
          "hgvs_p": "p.Arg183His",
          "transcript": "ENST00000890034.1",
          "protein_id": "ENSP00000560093.1",
          "transcript_support_level": null,
          "aa_start": 183,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 548,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890034.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AUP1",
          "gene_hgnc_id": 891,
          "hgvs_c": "c.545G>A",
          "hgvs_p": "p.Arg182His",
          "transcript": "ENST00000890031.1",
          "protein_id": "ENSP00000560090.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 545,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890031.1"
        },
        {
          "aa_ref": "R",
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      "dbsnp": "rs879539638",
      "frequency_reference_population": 0.000012391359,
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      "gnomad_exomes_af": 0.00000957671,
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      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7440836429595947,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2011,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.08,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 2.159,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_181575.5",
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          "effects": [
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          "inheritance_mode": "",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}