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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-75493327-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=75493327&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 75493327,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_032181.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "NM_001135032.2",
          "protein_id": "NP_001128504.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000393913.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001135032.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000393913.8",
          "protein_id": "ENSP00000377490.3",
          "transcript_support_level": 1,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001135032.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393913.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.425G>A",
          "hgvs_p": "p.Arg142Gln",
          "transcript": "ENST00000910300.1",
          "protein_id": "ENSP00000580359.1",
          "transcript_support_level": null,
          "aa_start": 142,
          "aa_end": null,
          "aa_length": 171,
          "cds_start": 425,
          "cds_end": null,
          "cds_length": 516,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910300.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.425G>A",
          "hgvs_p": "p.Arg142Gln",
          "transcript": "ENST00000910305.1",
          "protein_id": "ENSP00000580364.1",
          "transcript_support_level": null,
          "aa_start": 142,
          "aa_end": null,
          "aa_length": 171,
          "cds_start": 425,
          "cds_end": null,
          "cds_length": 516,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910305.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "NM_001369524.1",
          "protein_id": "NP_001356453.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001369524.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "NM_001369525.1",
          "protein_id": "NP_001356454.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001369525.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "NM_032181.3",
          "protein_id": "NP_115557.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032181.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000233712.5",
          "protein_id": "ENSP00000233712.1",
          "transcript_support_level": 2,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000233712.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000410071.5",
          "protein_id": "ENSP00000386930.1",
          "transcript_support_level": 3,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000410071.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000410113.5",
          "protein_id": "ENSP00000386435.1",
          "transcript_support_level": 3,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000410113.5"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910257.1",
          "protein_id": "ENSP00000580316.1",
          "transcript_support_level": null,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
          "cds_end": null,
          "cds_length": 459,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910257.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "EVA1A",
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          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910258.1",
          "protein_id": "ENSP00000580317.1",
          "transcript_support_level": null,
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          "aa_length": 152,
          "cds_start": 368,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000910258.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "EVA1A",
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          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910259.1",
          "protein_id": "ENSP00000580318.1",
          "transcript_support_level": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910260.1",
          "protein_id": "ENSP00000580319.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 152,
          "cds_start": 368,
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          "cds_length": 459,
          "cdna_start": null,
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          "cdna_length": null,
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        {
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          ],
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          "gene_symbol": "EVA1A",
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          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910261.1",
          "protein_id": "ENSP00000580320.1",
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          "cds_start": 368,
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          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000910261.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
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          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910262.1",
          "protein_id": "ENSP00000580321.1",
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          "aa_length": 152,
          "cds_start": 368,
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          "cdna_start": null,
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        {
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          ],
          "exon_rank": 5,
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          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910263.1",
          "protein_id": "ENSP00000580322.1",
          "transcript_support_level": null,
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        {
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          "gene_symbol": "EVA1A",
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        },
        {
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln",
          "transcript": "ENST00000910266.1",
          "protein_id": "ENSP00000580325.1",
          "transcript_support_level": null,
          "aa_start": 123,
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          "aa_length": 152,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000910266.1"
        },
        {
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        {
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          "gene_symbol": "EVA1A",
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          "hgvs_c": "n.521-23957G>A",
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          "biotype": "pseudogene",
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        {
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          "protein_coding": true,
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          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
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          "exon_count": 3,
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          "gene_symbol": "EVA1A",
          "gene_hgnc_id": 25816,
          "hgvs_c": "c.*65G>A",
          "hgvs_p": null,
          "transcript": "ENST00000452003.1",
          "protein_id": "ENSP00000388105.1",
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": 100,
          "cds_start": null,
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          "cds_length": 303,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000452003.1"
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      ],
      "gene_symbol": "EVA1A",
      "gene_hgnc_id": 25816,
      "dbsnp": "rs370520675",
      "frequency_reference_population": 0.0000136815,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 20,
      "gnomad_exomes_af": 0.0000136815,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 20,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.576988160610199,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.328,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4582,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.09,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.905,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_032181.3",
          "gene_symbol": "EVA1A",
          "hgnc_id": 25816,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.368G>A",
          "hgvs_p": "p.Arg123Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}