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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-97658089-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=97658089&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 97658089,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_005735.4",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.779A>C",
          "hgvs_p": "p.Glu260Ala",
          "transcript": "NM_005735.4",
          "protein_id": "NP_005726.1",
          "transcript_support_level": null,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 376,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 1131,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000289228.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005735.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.779A>C",
          "hgvs_p": "p.Glu260Ala",
          "transcript": "ENST00000289228.7",
          "protein_id": "ENSP00000289228.5",
          "transcript_support_level": 1,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 376,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 1131,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005735.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000289228.7"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.779A>C",
          "hgvs_p": "p.Glu260Ala",
          "transcript": "ENST00000855774.1",
          "protein_id": "ENSP00000525833.1",
          "transcript_support_level": null,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855774.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.812A>C",
          "hgvs_p": "p.Glu271Ala",
          "transcript": "ENST00000936776.1",
          "protein_id": "ENSP00000606835.1",
          "transcript_support_level": null,
          "aa_start": 271,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 812,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936776.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.797A>C",
          "hgvs_p": "p.Glu266Ala",
          "transcript": "ENST00000855778.1",
          "protein_id": "ENSP00000525837.1",
          "transcript_support_level": null,
          "aa_start": 266,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": 797,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855778.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.776A>C",
          "hgvs_p": "p.Glu259Ala",
          "transcript": "ENST00000936775.1",
          "protein_id": "ENSP00000606834.1",
          "transcript_support_level": null,
          "aa_start": 259,
          "aa_end": null,
          "aa_length": 375,
          "cds_start": 776,
          "cds_end": null,
          "cds_length": 1128,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936775.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.779A>C",
          "hgvs_p": "p.Glu260Ala",
          "transcript": "ENST00000855775.1",
          "protein_id": "ENSP00000525834.1",
          "transcript_support_level": null,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 369,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 1110,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855775.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.743A>C",
          "hgvs_p": "p.Glu248Ala",
          "transcript": "ENST00000855776.1",
          "protein_id": "ENSP00000525835.1",
          "transcript_support_level": null,
          "aa_start": 248,
          "aa_end": null,
          "aa_length": 364,
          "cds_start": 743,
          "cds_end": null,
          "cds_length": 1095,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855776.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.671A>C",
          "hgvs_p": "p.Glu224Ala",
          "transcript": "ENST00000936777.1",
          "protein_id": "ENSP00000606836.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 340,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 1023,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936777.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.638A>C",
          "hgvs_p": "p.Glu213Ala",
          "transcript": "ENST00000855773.1",
          "protein_id": "ENSP00000525832.1",
          "transcript_support_level": null,
          "aa_start": 213,
          "aa_end": null,
          "aa_length": 329,
          "cds_start": 638,
          "cds_end": null,
          "cds_length": 990,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855773.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.572A>C",
          "hgvs_p": "p.Glu191Ala",
          "transcript": "ENST00000855777.1",
          "protein_id": "ENSP00000525836.1",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 572,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000855777.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.437A>C",
          "hgvs_p": "p.Glu146Ala",
          "transcript": "ENST00000936778.1",
          "protein_id": "ENSP00000606837.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 262,
          "cds_start": 437,
          "cds_end": null,
          "cds_length": 789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000936778.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.647A>C",
          "hgvs_p": "p.Glu216Ala",
          "transcript": "XM_017003116.2",
          "protein_id": "XP_016858605.1",
          "transcript_support_level": null,
          "aa_start": 216,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 647,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017003116.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "c.557A>C",
          "hgvs_p": "p.Glu186Ala",
          "transcript": "XM_005263854.6",
          "protein_id": "XP_005263911.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 302,
          "cds_start": 557,
          "cds_end": null,
          "cds_length": 909,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005263854.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTR1B",
          "gene_hgnc_id": 168,
          "hgvs_c": "n.1040A>C",
          "hgvs_p": null,
          "transcript": "ENST00000451664.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000451664.1"
        }
      ],
      "gene_symbol": "ACTR1B",
      "gene_hgnc_id": 168,
      "dbsnp": "rs753284851",
      "frequency_reference_population": 0.0000049569735,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 8,
      "gnomad_exomes_af": 0.0000027365,
      "gnomad_genomes_af": 0.0000262867,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.97719806432724,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.4000000059604645,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.97,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.8218,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.59,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.014,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.4,
      "spliceai_max_prediction": "Uncertain_significance",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_005735.4",
          "gene_symbol": "ACTR1B",
          "hgnc_id": 168,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.779A>C",
          "hgvs_p": "p.Glu260Ala"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}