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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-99402317-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=99402317&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 99402317,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001321454.2",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3571G>A",
          "hgvs_p": "p.Val1191Met",
          "transcript": "NM_016316.4",
          "protein_id": "NP_057400.1",
          "transcript_support_level": null,
          "aa_start": 1191,
          "aa_end": null,
          "aa_length": 1251,
          "cds_start": 3571,
          "cds_end": null,
          "cds_length": 3756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000258428.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016316.4"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3571G>A",
          "hgvs_p": "p.Val1191Met",
          "transcript": "ENST00000258428.8",
          "protein_id": "ENSP00000258428.3",
          "transcript_support_level": 1,
          "aa_start": 1191,
          "aa_end": null,
          "aa_length": 1251,
          "cds_start": 3571,
          "cds_end": null,
          "cds_length": 3756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_016316.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000258428.8"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3568G>A",
          "hgvs_p": "p.Val1190Met",
          "transcript": "ENST00000393445.7",
          "protein_id": "ENSP00000377091.3",
          "transcript_support_level": 1,
          "aa_start": 1190,
          "aa_end": null,
          "aa_length": 1250,
          "cds_start": 3568,
          "cds_end": null,
          "cds_length": 3753,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393445.7"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3679G>A",
          "hgvs_p": "p.Val1227Met",
          "transcript": "NM_001321454.2",
          "protein_id": "NP_001308383.1",
          "transcript_support_level": null,
          "aa_start": 1227,
          "aa_end": null,
          "aa_length": 1287,
          "cds_start": 3679,
          "cds_end": null,
          "cds_length": 3864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001321454.2"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3679G>A",
          "hgvs_p": "p.Val1227Met",
          "transcript": "ENST00000879664.1",
          "protein_id": "ENSP00000549723.1",
          "transcript_support_level": null,
          "aa_start": 1227,
          "aa_end": null,
          "aa_length": 1287,
          "cds_start": 3679,
          "cds_end": null,
          "cds_length": 3864,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879664.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3676G>A",
          "hgvs_p": "p.Val1226Met",
          "transcript": "ENST00000879665.1",
          "protein_id": "ENSP00000549724.1",
          "transcript_support_level": null,
          "aa_start": 1226,
          "aa_end": null,
          "aa_length": 1286,
          "cds_start": 3676,
          "cds_end": null,
          "cds_length": 3861,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879665.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3571G>A",
          "hgvs_p": "p.Val1191Met",
          "transcript": "ENST00000879663.1",
          "protein_id": "ENSP00000549722.1",
          "transcript_support_level": null,
          "aa_start": 1191,
          "aa_end": null,
          "aa_length": 1251,
          "cds_start": 3571,
          "cds_end": null,
          "cds_length": 3756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879663.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3571G>A",
          "hgvs_p": "p.Val1191Met",
          "transcript": "ENST00000920279.1",
          "protein_id": "ENSP00000590338.1",
          "transcript_support_level": null,
          "aa_start": 1191,
          "aa_end": null,
          "aa_length": 1251,
          "cds_start": 3571,
          "cds_end": null,
          "cds_length": 3756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920279.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3568G>A",
          "hgvs_p": "p.Val1190Met",
          "transcript": "NM_001037872.3",
          "protein_id": "NP_001032961.1",
          "transcript_support_level": null,
          "aa_start": 1190,
          "aa_end": null,
          "aa_length": 1250,
          "cds_start": 3568,
          "cds_end": null,
          "cds_length": 3753,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001037872.3"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3568G>A",
          "hgvs_p": "p.Val1190Met",
          "transcript": "ENST00000920275.1",
          "protein_id": "ENSP00000590334.1",
          "transcript_support_level": null,
          "aa_start": 1190,
          "aa_end": null,
          "aa_length": 1250,
          "cds_start": 3568,
          "cds_end": null,
          "cds_length": 3753,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920275.1"
        },
        {
          "aa_ref": "V",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3568G>A",
          "hgvs_p": "p.Val1190Met",
          "transcript": "ENST00000949065.1",
          "protein_id": "ENSP00000619124.1",
          "transcript_support_level": null,
          "aa_start": 1190,
          "aa_end": null,
          "aa_length": 1250,
          "cds_start": 3568,
          "cds_end": null,
          "cds_length": 3753,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949065.1"
        },
        {
          "aa_ref": "V",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
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          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3538G>A",
          "hgvs_p": "p.Val1180Met",
          "transcript": "ENST00000920281.1",
          "protein_id": "ENSP00000590340.1",
          "transcript_support_level": null,
          "aa_start": 1180,
          "aa_end": null,
          "aa_length": 1240,
          "cds_start": 3538,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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        {
          "aa_ref": "V",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3460G>A",
          "hgvs_p": "p.Val1154Met",
          "transcript": "ENST00000920280.1",
          "protein_id": "ENSP00000590339.1",
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "V",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
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          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3415G>A",
          "hgvs_p": "p.Val1139Met",
          "transcript": "ENST00000879666.1",
          "protein_id": "ENSP00000549725.1",
          "transcript_support_level": null,
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          "aa_end": null,
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        {
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          "intron_rank": null,
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          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
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          "hgvs_p": "p.Val1120Met",
          "transcript": "NM_001321455.2",
          "protein_id": "NP_001308384.1",
          "transcript_support_level": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001321455.2"
        },
        {
          "aa_ref": "V",
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          "strand": false,
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          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3343G>A",
          "hgvs_p": "p.Val1115Met",
          "transcript": "ENST00000949066.1",
          "protein_id": "ENSP00000619125.1",
          "transcript_support_level": null,
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          "biotype": "protein_coding",
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        {
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          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.3190G>A",
          "hgvs_p": "p.Val1064Met",
          "transcript": "ENST00000920278.1",
          "protein_id": "ENSP00000590337.1",
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        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "REV1",
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          "hgvs_p": "p.Val846Met",
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.2536G>A",
          "hgvs_p": "p.Val846Met",
          "transcript": "ENST00000949067.1",
          "protein_id": "ENSP00000619126.1",
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          "aa_length": 906,
          "cds_start": 2536,
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          "cds_length": 2721,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949067.1"
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "c.2428G>A",
          "hgvs_p": "p.Val810Met",
          "transcript": "ENST00000920277.1",
          "protein_id": "ENSP00000590336.1",
          "transcript_support_level": null,
          "aa_start": 810,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 2428,
          "cds_end": null,
          "cds_length": 2613,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_135651.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "n.4079G>A",
          "hgvs_p": null,
          "transcript": "NR_135652.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_135652.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "n.3616G>A",
          "hgvs_p": null,
          "transcript": "NR_135653.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_135653.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "n.*3812G>A",
          "hgvs_p": null,
          "transcript": "ENST00000413697.5",
          "protein_id": "ENSP00000416274.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000413697.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "REV1",
          "gene_hgnc_id": 14060,
          "hgvs_c": "n.*213G>A",
          "hgvs_p": null,
          "transcript": "ENST00000465086.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000465086.1"
        }
      ],
      "gene_symbol": "REV1",
      "gene_hgnc_id": 14060,
      "dbsnp": "rs1455918852",
      "frequency_reference_population": 0.000042878917,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 66,
      "gnomad_exomes_af": 0.0000468609,
      "gnomad_genomes_af": 0.00000657315,
      "gnomad_exomes_ac": 65,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.45874810218811035,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.257,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.9506,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.1,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.526,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001321454.2",
          "gene_symbol": "REV1",
          "hgnc_id": 14060,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.3679G>A",
          "hgvs_p": "p.Val1227Met"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}