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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-10044403-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=10044403&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 10044403,
      "ref": "C",
      "alt": "T",
      "effect": "5_prime_UTR_premature_start_codon_gain_variant",
      "transcript": "NM_001303472.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.556C>T",
          "hgvs_p": "p.Arg186Cys",
          "transcript": "NM_022096.6",
          "protein_id": "NP_071379.3",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 776,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 2331,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000378392.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_022096.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.556C>T",
          "hgvs_p": "p.Arg186Cys",
          "transcript": "ENST00000378392.6",
          "protein_id": "ENSP00000367644.1",
          "transcript_support_level": 1,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 776,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 2331,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_022096.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378392.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.-12C>T",
          "hgvs_p": null,
          "transcript": "NM_001303472.2",
          "protein_id": "NP_001290401.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001303472.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.-12C>T",
          "hgvs_p": null,
          "transcript": "XM_047440366.1",
          "protein_id": "XP_047296322.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440366.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.-12C>T",
          "hgvs_p": null,
          "transcript": "XM_047440367.1",
          "protein_id": "XP_047296323.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440367.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.556C>T",
          "hgvs_p": "p.Arg186Cys",
          "transcript": "NM_198798.3",
          "protein_id": "NP_942093.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 776,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 2331,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_198798.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.556C>T",
          "hgvs_p": "p.Arg186Cys",
          "transcript": "ENST00000378380.4",
          "protein_id": "ENSP00000367631.3",
          "transcript_support_level": 2,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 776,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 2331,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378380.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.556C>T",
          "hgvs_p": "p.Arg186Cys",
          "transcript": "ENST00000937999.1",
          "protein_id": "ENSP00000608058.1",
          "transcript_support_level": null,
          "aa_start": 186,
          "aa_end": null,
          "aa_length": 776,
          "cds_start": 556,
          "cds_end": null,
          "cds_length": 2331,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000937999.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.-12C>T",
          "hgvs_p": null,
          "transcript": "NM_001303472.2",
          "protein_id": "NP_001290401.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001303472.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.-12C>T",
          "hgvs_p": null,
          "transcript": "XM_047440366.1",
          "protein_id": "XP_047296322.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440366.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.-12C>T",
          "hgvs_p": null,
          "transcript": "XM_047440367.1",
          "protein_id": "XP_047296323.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440367.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "c.546+1082C>T",
          "hgvs_p": null,
          "transcript": "ENST00000966174.1",
          "protein_id": "ENSP00000636233.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966174.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANKEF1",
          "gene_hgnc_id": 15803,
          "hgvs_c": "n.863C>T",
          "hgvs_p": null,
          "transcript": "ENST00000488991.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000488991.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "SNAP25-AS1",
          "gene_hgnc_id": 44312,
          "hgvs_c": "n.362-17755G>A",
          "hgvs_p": null,
          "transcript": "ENST00000421143.7",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000421143.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "SNAP25-AS1",
          "gene_hgnc_id": 44312,
          "hgvs_c": "n.748-17755G>A",
          "hgvs_p": null,
          "transcript": "ENST00000603542.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000603542.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SNAP25-AS1",
          "gene_hgnc_id": 44312,
          "hgvs_c": "n.629-17755G>A",
          "hgvs_p": null,
          "transcript": "ENST00000655307.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000655307.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SNAP25-AS1",
          "gene_hgnc_id": 44312,
          "hgvs_c": "n.209-17755G>A",
          "hgvs_p": null,
          "transcript": "ENST00000656496.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000656496.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SNAP25-AS1",
          "gene_hgnc_id": 44312,
          "hgvs_c": "n.610-17755G>A",
          "hgvs_p": null,
          "transcript": "ENST00000658443.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000658443.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "SNAP25-AS1",
          "gene_hgnc_id": 44312,
          "hgvs_c": "n.709-17755G>A",
          "hgvs_p": null,
          "transcript": "ENST00000660550.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000660550.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "SNAP25-AS1",
          "gene_hgnc_id": 44312,
          "hgvs_c": "n.533-17755G>A",
          "hgvs_p": null,
          "transcript": "ENST00000661614.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000661614.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
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          ],
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        {
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          ],
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          "gene_symbol": "SNAP25-AS1",
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          "biotype": "pseudogene",
          "feature": "ENST00000670580.1"
        },
        {
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            "intron_variant"
          ],
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          "exon_count": 5,
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          "gene_symbol": "SNAP25-AS1",
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          "hgvs_c": "n.500-17755G>A",
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          "transcript": "NR_040710.1",
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "pseudogene",
          "feature": "NR_040710.1"
        }
      ],
      "gene_symbol": "ANKEF1",
      "gene_hgnc_id": 15803,
      "dbsnp": "rs139757926",
      "frequency_reference_population": 0.000035321107,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 57,
      "gnomad_exomes_af": 0.0000335236,
      "gnomad_genomes_af": 0.0000525935,
      "gnomad_exomes_ac": 49,
      "gnomad_genomes_ac": 8,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6143791079521179,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.25,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2482,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.11,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.892,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001303472.2",
          "gene_symbol": "ANKEF1",
          "hgnc_id": 15803,
          "effects": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-12C>T",
          "hgvs_p": null
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000603542.6",
          "gene_symbol": "SNAP25-AS1",
          "hgnc_id": 44312,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.748-17755G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}