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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-13801652-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=13801652&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 13801652,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000378106.10",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.686T>C",
          "hgvs_p": "p.Leu229Pro",
          "transcript": "NM_024120.5",
          "protein_id": "NP_077025.2",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 686,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": 727,
          "cdna_end": null,
          "cdna_length": 5449,
          "mane_select": "ENST00000378106.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.686T>C",
          "hgvs_p": "p.Leu229Pro",
          "transcript": "ENST00000378106.10",
          "protein_id": "ENSP00000367346.5",
          "transcript_support_level": 1,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 686,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": 727,
          "cdna_end": null,
          "cdna_length": 5449,
          "mane_select": "NM_024120.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.602T>C",
          "hgvs_p": "p.Leu201Pro",
          "transcript": "ENST00000463598.1",
          "protein_id": "ENSP00000420497.1",
          "transcript_support_level": 1,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 602,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": 621,
          "cdna_end": null,
          "cdna_length": 991,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.602T>C",
          "hgvs_p": "p.Leu201Pro",
          "transcript": "NM_001039375.3",
          "protein_id": "NP_001034464.1",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 602,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": 643,
          "cdna_end": null,
          "cdna_length": 5365,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.686T>C",
          "hgvs_p": "p.Leu229Pro",
          "transcript": "NM_001352408.2",
          "protein_id": "NP_001339337.1",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 247,
          "cds_start": 686,
          "cds_end": null,
          "cds_length": 744,
          "cdna_start": 727,
          "cdna_end": null,
          "cdna_length": 1081,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.215T>C",
          "hgvs_p": "p.Leu72Pro",
          "transcript": "NM_001352403.2",
          "protein_id": "NP_001339332.1",
          "transcript_support_level": null,
          "aa_start": 72,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 215,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": 623,
          "cdna_end": null,
          "cdna_length": 5345,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.125T>C",
          "hgvs_p": "p.Leu42Pro",
          "transcript": "NM_001352406.2",
          "protein_id": "NP_001339335.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 125,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": 747,
          "cdna_end": null,
          "cdna_length": 5469,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.125T>C",
          "hgvs_p": "p.Leu42Pro",
          "transcript": "NM_001352407.2",
          "protein_id": "NP_001339336.1",
          "transcript_support_level": null,
          "aa_start": 42,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 125,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": 861,
          "cdna_end": null,
          "cdna_length": 5583,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.686T>C",
          "hgvs_p": "p.Leu229Pro",
          "transcript": "XM_047440469.1",
          "protein_id": "XP_047296425.1",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 686,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": 727,
          "cdna_end": null,
          "cdna_length": 5365,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.686T>C",
          "hgvs_p": "p.Leu229Pro",
          "transcript": "XM_011529342.3",
          "protein_id": "XP_011527644.1",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 299,
          "cds_start": 686,
          "cds_end": null,
          "cds_length": 900,
          "cdna_start": 727,
          "cdna_end": null,
          "cdna_length": 1025,
          "mane_select": null,
          "mane_plus": null,
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          "feature": null
        },
        {
          "aa_ref": "L",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.602T>C",
          "hgvs_p": "p.Leu201Pro",
          "transcript": "XM_047440470.1",
          "protein_id": "XP_047296426.1",
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          "aa_start": 201,
          "aa_end": null,
          "aa_length": 219,
          "cds_start": 602,
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          "cds_length": 660,
          "cdna_start": 643,
          "cdna_end": null,
          "cdna_length": 791,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "NDUFAF5",
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          "hgvs_c": "c.215T>C",
          "hgvs_p": "p.Leu72Pro",
          "transcript": "XM_006723624.3",
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          "cdna_start": 757,
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        {
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          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": 6,
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "NDUFAF5",
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          "hgvs_c": "c.215T>C",
          "hgvs_p": "p.Leu72Pro",
          "transcript": "XM_047440471.1",
          "protein_id": "XP_047296427.1",
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          "cds_start": 215,
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        },
        {
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 6,
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          "intron_rank": null,
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          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.125T>C",
          "hgvs_p": "p.Leu42Pro",
          "transcript": "XM_047440472.1",
          "protein_id": "XP_047296428.1",
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          "cdna_start": 1446,
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        },
        {
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          ],
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          "intron_rank": null,
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          "hgvs_c": "c.125T>C",
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        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.686T>C",
          "hgvs_p": null,
          "transcript": "ENST00000378081.9",
          "protein_id": "ENSP00000437325.1",
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          "aa_start": null,
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        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.359T>C",
          "hgvs_p": null,
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          "cdna_length": 550,
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        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
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          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "NDUFAF5",
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          "hgvs_c": "n.563T>C",
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        },
        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
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          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.85T>C",
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          "mane_select": null,
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        },
        {
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          "canonical": false,
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          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
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          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.646T>C",
          "hgvs_p": null,
          "transcript": "ENST00000476536.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
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          "aa_length": null,
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          "cdna_start": null,
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          "cdna_length": 763,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.207T>C",
          "hgvs_p": null,
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      ],
      "gene_symbol": "NDUFAF5",
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      "dbsnp": "rs118203929",
      "frequency_reference_population": 0.000008054343,
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      "gnomad_exomes_af": 0.00000820888,
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      "gnomad_exomes_ac": 12,
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      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.9795644283294678,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.953,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9837,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.42,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.202,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "apogee2_score": null,
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      "acmg_score": 14,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PP3_Strong,PP5_Very_Strong",
      "acmg_by_gene": [
        {
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          "benign_score": 0,
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          "criteria": [
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            "PP3_Strong",
            "PP5_Very_Strong"
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          "verdict": "Pathogenic",
          "transcript": "ENST00000378106.10",
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          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.686T>C",
          "hgvs_p": "p.Leu229Pro"
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      ],
      "clinvar_disease": " nuclear type 16,Mitochondrial complex I deficiency,not provided",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LP:4",
      "phenotype_combined": "Mitochondrial complex I deficiency, nuclear type 16|not provided|Mitochondrial complex I deficiency",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
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  ],
  "message": null
}