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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-13816520-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=13816520&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 13816520,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_024120.5",
      "consequences": [
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.836T>A",
          "hgvs_p": "p.Met279Lys",
          "transcript": "NM_024120.5",
          "protein_id": "NP_077025.2",
          "transcript_support_level": null,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 836,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000378106.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024120.5"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.836T>A",
          "hgvs_p": "p.Met279Lys",
          "transcript": "ENST00000378106.10",
          "protein_id": "ENSP00000367346.5",
          "transcript_support_level": 1,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 836,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024120.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378106.10"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.752T>A",
          "hgvs_p": "p.Met251Lys",
          "transcript": "ENST00000463598.1",
          "protein_id": "ENSP00000420497.1",
          "transcript_support_level": 1,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000463598.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.962T>A",
          "hgvs_p": "p.Met321Lys",
          "transcript": "ENST00000874783.1",
          "protein_id": "ENSP00000544842.1",
          "transcript_support_level": null,
          "aa_start": 321,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 962,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874783.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.806T>A",
          "hgvs_p": "p.Met269Lys",
          "transcript": "ENST00000934787.1",
          "protein_id": "ENSP00000604846.1",
          "transcript_support_level": null,
          "aa_start": 269,
          "aa_end": null,
          "aa_length": 335,
          "cds_start": 806,
          "cds_end": null,
          "cds_length": 1008,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000934787.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.752T>A",
          "hgvs_p": "p.Met251Lys",
          "transcript": "NM_001039375.3",
          "protein_id": "NP_001034464.1",
          "transcript_support_level": null,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 752,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001039375.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.746T>A",
          "hgvs_p": "p.Met249Lys",
          "transcript": "ENST00000949288.1",
          "protein_id": "ENSP00000619347.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 746,
          "cds_end": null,
          "cds_length": 948,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000949288.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.446T>A",
          "hgvs_p": "p.Met149Lys",
          "transcript": "ENST00000874782.1",
          "protein_id": "ENSP00000544841.1",
          "transcript_support_level": null,
          "aa_start": 149,
          "aa_end": null,
          "aa_length": 215,
          "cds_start": 446,
          "cds_end": null,
          "cds_length": 648,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874782.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.365T>A",
          "hgvs_p": "p.Met122Lys",
          "transcript": "NM_001352403.2",
          "protein_id": "NP_001339332.1",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 365,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352403.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.275T>A",
          "hgvs_p": "p.Met92Lys",
          "transcript": "NM_001352406.2",
          "protein_id": "NP_001339335.1",
          "transcript_support_level": null,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352406.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.275T>A",
          "hgvs_p": "p.Met92Lys",
          "transcript": "NM_001352407.2",
          "protein_id": "NP_001339336.1",
          "transcript_support_level": null,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352407.2"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.365T>A",
          "hgvs_p": "p.Met122Lys",
          "transcript": "XM_006723624.3",
          "protein_id": "XP_006723687.1",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 365,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006723624.3"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.365T>A",
          "hgvs_p": "p.Met122Lys",
          "transcript": "XM_047440471.1",
          "protein_id": "XP_047296427.1",
          "transcript_support_level": null,
          "aa_start": 122,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 365,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440471.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.275T>A",
          "hgvs_p": "p.Met92Lys",
          "transcript": "XM_047440472.1",
          "protein_id": "XP_047296428.1",
          "transcript_support_level": null,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440472.1"
        },
        {
          "aa_ref": "M",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.275T>A",
          "hgvs_p": "p.Met92Lys",
          "transcript": "XM_047440473.1",
          "protein_id": "XP_047296429.1",
          "transcript_support_level": null,
          "aa_start": 92,
          "aa_end": null,
          "aa_length": 158,
          "cds_start": 275,
          "cds_end": null,
          "cds_length": 477,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440473.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "c.779-355T>A",
          "hgvs_p": null,
          "transcript": "XM_047440469.1",
          "protein_id": "XP_047296425.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 954,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440469.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.*130T>A",
          "hgvs_p": null,
          "transcript": "ENST00000378081.9",
          "protein_id": "ENSP00000437325.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000378081.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.713T>A",
          "hgvs_p": null,
          "transcript": "ENST00000475968.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000475968.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.497T>A",
          "hgvs_p": null,
          "transcript": "ENST00000476200.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000476200.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NDUFAF5",
          "gene_hgnc_id": 15899,
          "hgvs_c": "n.456T>A",
          "hgvs_p": null,
          "transcript": "ENST00000479682.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000479682.5"
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      "computational_score_selected": 0.9058735966682434,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
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      "bayesdelnoaf_prediction": "Pathogenic",
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      "phylop100way_prediction": "Uncertain_significance",
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      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PM5,PP3_Moderate",
      "acmg_by_gene": [
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            "PM5",
            "PP3_Moderate"
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          "verdict": "Likely_pathogenic",
          "transcript": "NM_024120.5",
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          "hgvs_p": "p.Met279Lys"
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      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}