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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-20412138-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=20412138&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 0,
          "criteria": [
            "PM2"
          ],
          "effects": [
            "stop_gained"
          ],
          "gene_symbol": "RALGAPA2",
          "hgnc_id": 16207,
          "hgvs_c": "c.5506C>T",
          "hgvs_p": "p.Arg1836*",
          "inheritance_mode": "AR",
          "pathogenic_score": 2,
          "score": 2,
          "transcript": "NM_020343.4",
          "verdict": "Uncertain_significance"
        }
      ],
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_score": 2,
      "allele_count_reference_population": 2,
      "alphamissense_prediction": null,
      "alphamissense_score": null,
      "alt": "A",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.59,
      "chr": "20",
      "clinvar_classification": "Uncertain significance",
      "clinvar_disease": "not provided",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "computational_prediction_selected": "Pathogenic",
      "computational_score_selected": 0.5899999737739563,
      "computational_source_selected": "BayesDel_noAF",
      "consequences": [
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1873,
          "aa_ref": "R",
          "aa_start": 1836,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9545,
          "cdna_start": 5670,
          "cds_end": null,
          "cds_length": 5622,
          "cds_start": 5506,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 40,
          "exon_rank": 38,
          "exon_rank_end": null,
          "feature": "NM_020343.4",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5506C>T",
          "hgvs_p": "p.Arg1836*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000202677.12",
          "protein_coding": true,
          "protein_id": "NP_065076.2",
          "strand": false,
          "transcript": "NM_020343.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1873,
          "aa_ref": "R",
          "aa_start": 1836,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 9545,
          "cdna_start": 5670,
          "cds_end": null,
          "cds_length": 5622,
          "cds_start": 5506,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 40,
          "exon_rank": 38,
          "exon_rank_end": null,
          "feature": "ENST00000202677.12",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5506C>T",
          "hgvs_p": "p.Arg1836*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_020343.4",
          "protein_coding": true,
          "protein_id": "ENSP00000202677.6",
          "strand": false,
          "transcript": "ENST00000202677.12",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1860,
          "aa_ref": "R",
          "aa_start": 1823,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 6850,
          "cdna_start": 5619,
          "cds_end": null,
          "cds_length": 5583,
          "cds_start": 5467,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 40,
          "exon_rank": 38,
          "exon_rank_end": null,
          "feature": "ENST00000909985.1",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5467C>T",
          "hgvs_p": "p.Arg1823*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000580044.1",
          "strand": false,
          "transcript": "ENST00000909985.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1827,
          "aa_ref": "R",
          "aa_start": 1790,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9372,
          "cdna_start": 5519,
          "cds_end": null,
          "cds_length": 5484,
          "cds_start": 5368,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 39,
          "exon_rank": 37,
          "exon_rank_end": null,
          "feature": "ENST00000934890.1",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5368C>T",
          "hgvs_p": "p.Arg1790*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000604949.1",
          "strand": false,
          "transcript": "ENST00000934890.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1739,
          "aa_ref": "R",
          "aa_start": 1652,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 8769,
          "cdna_start": 4956,
          "cds_end": null,
          "cds_length": 5220,
          "cds_start": 4954,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 33,
          "exon_rank": 32,
          "exon_rank_end": null,
          "feature": "ENST00000430436.5",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.4954C>T",
          "hgvs_p": "p.Arg1652*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000400085.1",
          "strand": false,
          "transcript": "ENST00000430436.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1970,
          "aa_ref": "R",
          "aa_start": 1883,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9646,
          "cdna_start": 5811,
          "cds_end": null,
          "cds_length": 5913,
          "cds_start": 5647,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 40,
          "exon_rank": 39,
          "exon_rank_end": null,
          "feature": "XM_006723598.2",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5647C>T",
          "hgvs_p": "p.Arg1883*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_006723661.1",
          "strand": false,
          "transcript": "XM_006723598.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1923,
          "aa_ref": "R",
          "aa_start": 1836,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9505,
          "cdna_start": 5670,
          "cds_end": null,
          "cds_length": 5772,
          "cds_start": 5506,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 39,
          "exon_rank": 38,
          "exon_rank_end": null,
          "feature": "XM_005260768.3",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5506C>T",
          "hgvs_p": "p.Arg1836*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_005260825.1",
          "strand": false,
          "transcript": "XM_005260768.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1920,
          "aa_ref": "R",
          "aa_start": 1883,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9686,
          "cdna_start": 5811,
          "cds_end": null,
          "cds_length": 5763,
          "cds_start": 5647,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 41,
          "exon_rank": 39,
          "exon_rank_end": null,
          "feature": "XM_006723599.2",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5647C>T",
          "hgvs_p": "p.Arg1883*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_006723662.1",
          "strand": false,
          "transcript": "XM_006723599.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1808,
          "aa_ref": "R",
          "aa_start": 1721,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9482,
          "cdna_start": 5647,
          "cds_end": null,
          "cds_length": 5427,
          "cds_start": 5161,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 38,
          "exon_rank": 37,
          "exon_rank_end": null,
          "feature": "XM_011529310.3",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.5161C>T",
          "hgvs_p": "p.Arg1721*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_011527612.1",
          "strand": false,
          "transcript": "XM_011529310.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1371,
          "aa_ref": "R",
          "aa_start": 1284,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 7761,
          "cdna_start": 3926,
          "cds_end": null,
          "cds_length": 4116,
          "cds_start": 3850,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 27,
          "exon_rank": 26,
          "exon_rank_end": null,
          "feature": "XM_047440322.1",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.3850C>T",
          "hgvs_p": "p.Arg1284*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_047296278.1",
          "strand": false,
          "transcript": "XM_047440322.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1344,
          "aa_ref": "R",
          "aa_start": 1257,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 10094,
          "cdna_start": 6259,
          "cds_end": null,
          "cds_length": 4035,
          "cds_start": 3769,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 26,
          "exon_rank": 25,
          "exon_rank_end": null,
          "feature": "XM_011529311.3",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.3769C>T",
          "hgvs_p": "p.Arg1257*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_011527613.2",
          "strand": false,
          "transcript": "XM_011529311.3",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1324,
          "aa_ref": "R",
          "aa_start": 1237,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 7692,
          "cdna_start": 3857,
          "cds_end": null,
          "cds_length": 3975,
          "cds_start": 3709,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 26,
          "exon_rank": 25,
          "exon_rank_end": null,
          "feature": "XM_047440323.1",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.3709C>T",
          "hgvs_p": "p.Arg1237*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_047296279.1",
          "strand": false,
          "transcript": "XM_047440323.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "*",
          "aa_end": null,
          "aa_length": 1297,
          "aa_ref": "R",
          "aa_start": 1210,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9952,
          "cdna_start": 6117,
          "cds_end": null,
          "cds_length": 3894,
          "cds_start": 3628,
          "consequences": [
            "stop_gained"
          ],
          "exon_count": 25,
          "exon_rank": 24,
          "exon_rank_end": null,
          "feature": "XM_047440324.1",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "c.3628C>T",
          "hgvs_p": "p.Arg1210*",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_047296280.1",
          "strand": false,
          "transcript": "XM_047440324.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 11324,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 42,
          "exon_rank": 39,
          "exon_rank_end": null,
          "feature": "XR_001754352.2",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "n.5811C>T",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "XR_001754352.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 10068,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 43,
          "exon_rank": 39,
          "exon_rank_end": null,
          "feature": "XR_007067470.1",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "n.5811C>T",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "XR_007067470.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 11183,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 41,
          "exon_rank": 38,
          "exon_rank_end": null,
          "feature": "XR_007067471.1",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "n.5670C>T",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "XR_007067471.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 9970,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 42,
          "exon_rank": 39,
          "exon_rank_end": null,
          "feature": "XR_937111.3",
          "gene_hgnc_id": 16207,
          "gene_symbol": "RALGAPA2",
          "hgvs_c": "n.5811C>T",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "XR_937111.3",
          "transcript_support_level": null
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs2060075185",
      "effect": "stop_gained",
      "frequency_reference_population": 0.0000013683728,
      "gene_hgnc_id": 16207,
      "gene_symbol": "RALGAPA2",
      "gnomad_exomes_ac": 2,
      "gnomad_exomes_af": 0.00000136837,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": null,
      "gnomad_genomes_af": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Uncertain significance",
      "phenotype_combined": "not provided",
      "phylop100way_prediction": "Benign",
      "phylop100way_score": 1.109,
      "pos": 20412138,
      "ref": "G",
      "revel_prediction": null,
      "revel_score": null,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0.05999999865889549,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0.06,
      "transcript": "NM_020343.4"
    }
  ]
}
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