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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-25009322-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=25009322&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 25009322,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_032501.4",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1838A>G",
          "hgvs_p": "p.Lys613Arg",
          "transcript": "NM_032501.4",
          "protein_id": "NP_115890.2",
          "transcript_support_level": null,
          "aa_start": 613,
          "aa_end": null,
          "aa_length": 689,
          "cds_start": 1838,
          "cds_end": null,
          "cds_length": 2070,
          "cdna_start": 1875,
          "cdna_end": null,
          "cdna_length": 3632,
          "mane_select": "ENST00000323482.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032501.4"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1838A>G",
          "hgvs_p": "p.Lys613Arg",
          "transcript": "ENST00000323482.9",
          "protein_id": "ENSP00000316924.4",
          "transcript_support_level": 1,
          "aa_start": 613,
          "aa_end": null,
          "aa_length": 689,
          "cds_start": 1838,
          "cds_end": null,
          "cds_length": 2070,
          "cdna_start": 1875,
          "cdna_end": null,
          "cdna_length": 3632,
          "mane_select": "NM_032501.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000323482.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "n.3005A>G",
          "hgvs_p": null,
          "transcript": "ENST00000484396.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4767,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000484396.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1931A>G",
          "hgvs_p": "p.Lys644Arg",
          "transcript": "ENST00000964866.1",
          "protein_id": "ENSP00000634925.1",
          "transcript_support_level": null,
          "aa_start": 644,
          "aa_end": null,
          "aa_length": 720,
          "cds_start": 1931,
          "cds_end": null,
          "cds_length": 2163,
          "cdna_start": 2060,
          "cdna_end": null,
          "cdna_length": 3820,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964866.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1832A>G",
          "hgvs_p": "p.Lys611Arg",
          "transcript": "NM_001252675.2",
          "protein_id": "NP_001239604.1",
          "transcript_support_level": null,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 687,
          "cds_start": 1832,
          "cds_end": null,
          "cds_length": 2064,
          "cdna_start": 1869,
          "cdna_end": null,
          "cdna_length": 3626,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001252675.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1832A>G",
          "hgvs_p": "p.Lys611Arg",
          "transcript": "ENST00000887164.1",
          "protein_id": "ENSP00000557223.1",
          "transcript_support_level": null,
          "aa_start": 611,
          "aa_end": null,
          "aa_length": 687,
          "cds_start": 1832,
          "cds_end": null,
          "cds_length": 2064,
          "cdna_start": 2375,
          "cdna_end": null,
          "cdna_length": 4137,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887164.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1820A>G",
          "hgvs_p": "p.Lys607Arg",
          "transcript": "ENST00000887168.1",
          "protein_id": "ENSP00000557227.1",
          "transcript_support_level": null,
          "aa_start": 607,
          "aa_end": null,
          "aa_length": 683,
          "cds_start": 1820,
          "cds_end": null,
          "cds_length": 2052,
          "cdna_start": 1890,
          "cdna_end": null,
          "cdna_length": 3650,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887168.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1802A>G",
          "hgvs_p": "p.Lys601Arg",
          "transcript": "ENST00000887172.1",
          "protein_id": "ENSP00000557231.1",
          "transcript_support_level": null,
          "aa_start": 601,
          "aa_end": null,
          "aa_length": 677,
          "cds_start": 1802,
          "cds_end": null,
          "cds_length": 2034,
          "cdna_start": 1843,
          "cdna_end": null,
          "cdna_length": 3598,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887172.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1745A>G",
          "hgvs_p": "p.Lys582Arg",
          "transcript": "ENST00000887171.1",
          "protein_id": "ENSP00000557230.1",
          "transcript_support_level": null,
          "aa_start": 582,
          "aa_end": null,
          "aa_length": 658,
          "cds_start": 1745,
          "cds_end": null,
          "cds_length": 1977,
          "cdna_start": 1782,
          "cdna_end": null,
          "cdna_length": 3542,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887171.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1700A>G",
          "hgvs_p": "p.Lys567Arg",
          "transcript": "ENST00000887166.1",
          "protein_id": "ENSP00000557225.1",
          "transcript_support_level": null,
          "aa_start": 567,
          "aa_end": null,
          "aa_length": 643,
          "cds_start": 1700,
          "cds_end": null,
          "cds_length": 1932,
          "cdna_start": 1797,
          "cdna_end": null,
          "cdna_length": 3559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887166.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1685A>G",
          "hgvs_p": "p.Lys562Arg",
          "transcript": "ENST00000887167.1",
          "protein_id": "ENSP00000557226.1",
          "transcript_support_level": null,
          "aa_start": 562,
          "aa_end": null,
          "aa_length": 638,
          "cds_start": 1685,
          "cds_end": null,
          "cds_length": 1917,
          "cdna_start": 1758,
          "cdna_end": null,
          "cdna_length": 3520,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887167.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1679A>G",
          "hgvs_p": "p.Lys560Arg",
          "transcript": "ENST00000964869.1",
          "protein_id": "ENSP00000634928.1",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 636,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1911,
          "cdna_start": 1720,
          "cdna_end": null,
          "cdna_length": 3482,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964869.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1670A>G",
          "hgvs_p": "p.Lys557Arg",
          "transcript": "ENST00000887169.1",
          "protein_id": "ENSP00000557228.1",
          "transcript_support_level": null,
          "aa_start": 557,
          "aa_end": null,
          "aa_length": 633,
          "cds_start": 1670,
          "cds_end": null,
          "cds_length": 1902,
          "cdna_start": 1711,
          "cdna_end": null,
          "cdna_length": 3473,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887169.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1667A>G",
          "hgvs_p": "p.Lys556Arg",
          "transcript": "ENST00000964867.1",
          "protein_id": "ENSP00000634926.1",
          "transcript_support_level": null,
          "aa_start": 556,
          "aa_end": null,
          "aa_length": 632,
          "cds_start": 1667,
          "cds_end": null,
          "cds_length": 1899,
          "cdna_start": 1748,
          "cdna_end": null,
          "cdna_length": 3510,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964867.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1583A>G",
          "hgvs_p": "p.Lys528Arg",
          "transcript": "ENST00000887170.1",
          "protein_id": "ENSP00000557229.1",
          "transcript_support_level": null,
          "aa_start": 528,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1583,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": 1620,
          "cdna_end": null,
          "cdna_length": 3381,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887170.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1541A>G",
          "hgvs_p": "p.Lys514Arg",
          "transcript": "ENST00000964870.1",
          "protein_id": "ENSP00000634929.1",
          "transcript_support_level": null,
          "aa_start": 514,
          "aa_end": null,
          "aa_length": 590,
          "cds_start": 1541,
          "cds_end": null,
          "cds_length": 1773,
          "cdna_start": 1581,
          "cdna_end": null,
          "cdna_length": 3335,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964870.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1475A>G",
          "hgvs_p": "p.Lys492Arg",
          "transcript": "NM_001252676.2",
          "protein_id": "NP_001239605.1",
          "transcript_support_level": null,
          "aa_start": 492,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 1475,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": 1745,
          "cdna_end": null,
          "cdna_length": 3502,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001252676.2"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1475A>G",
          "hgvs_p": "p.Lys492Arg",
          "transcript": "ENST00000537502.5",
          "protein_id": "ENSP00000439304.2",
          "transcript_support_level": 2,
          "aa_start": 492,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 1475,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": 1745,
          "cdna_end": null,
          "cdna_length": 3509,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000537502.5"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1223A>G",
          "hgvs_p": "p.Lys408Arg",
          "transcript": "ENST00000887165.1",
          "protein_id": "ENSP00000557224.1",
          "transcript_support_level": null,
          "aa_start": 408,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 1223,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": 1508,
          "cdna_end": null,
          "cdna_length": 3270,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887165.1"
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACSS1",
          "gene_hgnc_id": 16091,
          "hgvs_c": "c.1217A>G",
          "hgvs_p": "p.Lys406Arg",
          "transcript": "ENST00000964868.1",
          "protein_id": "ENSP00000634927.1",
          "transcript_support_level": null,
          "aa_start": 406,
          "aa_end": null,
          "aa_length": 482,
          "cds_start": 1217,
          "cds_end": null,
          "cds_length": 1449,
          "cdna_start": 1289,
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      "computational_score_selected": 0.031499773263931274,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "acmg_classification": "Likely_benign",
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            "BP6_Moderate"
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          "verdict": "Likely_benign",
          "transcript": "NM_032501.4",
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        {
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          "criteria": [
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            "BP4_Strong",
            "BP6_Moderate"
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          "verdict": "Likely_benign",
          "transcript": "ENST00000818190.1",
          "gene_symbol": "ENSG00000306411",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
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          "inheritance_mode": "",
          "hgvs_c": "n.221T>C",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
For research and educational, non-commercial use only. Not for clinical or diagnostic use. GeneBe does not provide medical advice. Data use for AI modeling is prohibited: if used, the cost is $0.001 per byte of downloaded uncompressed data.