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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-3227839-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=3227839&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 3227839,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001174090.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2576G>A",
          "hgvs_p": "p.Arg859Gln",
          "transcript": "NM_001174089.2",
          "protein_id": "NP_001167560.1",
          "transcript_support_level": null,
          "aa_start": 859,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 2576,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000642402.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001174089.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2576G>A",
          "hgvs_p": "p.Arg859Gln",
          "transcript": "ENST00000642402.1",
          "protein_id": "ENSP00000493503.1",
          "transcript_support_level": null,
          "aa_start": 859,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 2576,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001174089.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642402.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2624G>A",
          "hgvs_p": "p.Arg875Gln",
          "transcript": "ENST00000380056.7",
          "protein_id": "ENSP00000369396.3",
          "transcript_support_level": 1,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 2624,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380056.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2705G>A",
          "hgvs_p": "p.Arg902Gln",
          "transcript": "NM_001174090.2",
          "protein_id": "NP_001167561.1",
          "transcript_support_level": null,
          "aa_start": 902,
          "aa_end": null,
          "aa_length": 918,
          "cds_start": 2705,
          "cds_end": null,
          "cds_length": 2757,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001174090.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2705G>A",
          "hgvs_p": "p.Arg902Gln",
          "transcript": "ENST00000380059.7",
          "protein_id": "ENSP00000369399.3",
          "transcript_support_level": 2,
          "aa_start": 902,
          "aa_end": null,
          "aa_length": 918,
          "cds_start": 2705,
          "cds_end": null,
          "cds_length": 2757,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380059.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2624G>A",
          "hgvs_p": "p.Arg875Gln",
          "transcript": "NM_032034.4",
          "protein_id": "NP_114423.1",
          "transcript_support_level": null,
          "aa_start": 875,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 2624,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032034.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2591G>A",
          "hgvs_p": "p.Arg864Gln",
          "transcript": "NM_001400280.1",
          "protein_id": "NP_001387209.1",
          "transcript_support_level": null,
          "aa_start": 864,
          "aa_end": null,
          "aa_length": 880,
          "cds_start": 2591,
          "cds_end": null,
          "cds_length": 2643,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400280.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2570G>A",
          "hgvs_p": "p.Arg857Gln",
          "transcript": "ENST00000925552.1",
          "protein_id": "ENSP00000595611.1",
          "transcript_support_level": null,
          "aa_start": 857,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 2570,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925552.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2519G>A",
          "hgvs_p": "p.Arg840Gln",
          "transcript": "NM_001400277.1",
          "protein_id": "NP_001387206.1",
          "transcript_support_level": null,
          "aa_start": 840,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 2519,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400277.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2519G>A",
          "hgvs_p": "p.Arg840Gln",
          "transcript": "NM_001400278.1",
          "protein_id": "NP_001387207.1",
          "transcript_support_level": null,
          "aa_start": 840,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 2519,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400278.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2519G>A",
          "hgvs_p": "p.Arg840Gln",
          "transcript": "NM_001400279.1",
          "protein_id": "NP_001387208.1",
          "transcript_support_level": null,
          "aa_start": 840,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 2519,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400279.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2507G>A",
          "hgvs_p": "p.Arg836Gln",
          "transcript": "ENST00000644011.1",
          "protein_id": "ENSP00000496214.1",
          "transcript_support_level": null,
          "aa_start": 836,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 2507,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000644011.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2504G>A",
          "hgvs_p": "p.Arg835Gln",
          "transcript": "ENST00000876657.1",
          "protein_id": "ENSP00000546716.1",
          "transcript_support_level": null,
          "aa_start": 835,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 2504,
          "cds_end": null,
          "cds_length": 2556,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876657.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2504G>A",
          "hgvs_p": "p.Arg835Gln",
          "transcript": "ENST00000925551.1",
          "protein_id": "ENSP00000595610.1",
          "transcript_support_level": null,
          "aa_start": 835,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 2504,
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          "cds_length": 2556,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925551.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2501G>A",
          "hgvs_p": "p.Arg834Gln",
          "transcript": "ENST00000876658.1",
          "protein_id": "ENSP00000546717.1",
          "transcript_support_level": null,
          "aa_start": 834,
          "aa_end": null,
          "aa_length": 850,
          "cds_start": 2501,
          "cds_end": null,
          "cds_length": 2553,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876658.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2462G>A",
          "hgvs_p": "p.Arg821Gln",
          "transcript": "NM_001363745.2",
          "protein_id": "NP_001350674.1",
          "transcript_support_level": null,
          "aa_start": 821,
          "aa_end": null,
          "aa_length": 837,
          "cds_start": 2462,
          "cds_end": null,
          "cds_length": 2514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363745.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2462G>A",
          "hgvs_p": "p.Arg821Gln",
          "transcript": "ENST00000647296.1",
          "protein_id": "ENSP00000495050.1",
          "transcript_support_level": null,
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          "cds_length": 2514,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000647296.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2456G>A",
          "hgvs_p": "p.Arg819Gln",
          "transcript": "ENST00000876659.1",
          "protein_id": "ENSP00000546718.1",
          "transcript_support_level": null,
          "aa_start": 819,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 2456,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000876659.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2432G>A",
          "hgvs_p": "p.Arg811Gln",
          "transcript": "ENST00000925550.1",
          "protein_id": "ENSP00000595609.1",
          "transcript_support_level": null,
          "aa_start": 811,
          "aa_end": null,
          "aa_length": 827,
          "cds_start": 2432,
          "cds_end": null,
          "cds_length": 2484,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925550.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2375G>A",
          "hgvs_p": "p.Arg792Gln",
          "transcript": "ENST00000644692.1",
          "protein_id": "ENSP00000493824.1",
          "transcript_support_level": null,
          "aa_start": 792,
          "aa_end": null,
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      ],
      "gene_symbol": "SLC4A11",
      "gene_hgnc_id": 16438,
      "dbsnp": "rs751709036",
      "frequency_reference_population": 0.000018599714,
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      "allele_count_reference_population": 30,
      "gnomad_exomes_af": 0.000018483,
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      "gnomad_exomes_ac": 27,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.18119576573371887,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.241,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0801,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.29,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.293,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001174090.2",
          "gene_symbol": "SLC4A11",
          "hgnc_id": 16438,
          "effects": [
            "missense_variant"
          ],
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          "hgvs_p": "p.Arg902Gln"
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      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}