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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-3228968-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=3228968&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 3228968,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001174090.2",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2062G>A",
          "hgvs_p": "p.Ala688Thr",
          "transcript": "NM_001174089.2",
          "protein_id": "NP_001167560.1",
          "transcript_support_level": null,
          "aa_start": 688,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 2062,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000642402.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001174089.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2062G>A",
          "hgvs_p": "p.Ala688Thr",
          "transcript": "ENST00000642402.1",
          "protein_id": "ENSP00000493503.1",
          "transcript_support_level": null,
          "aa_start": 688,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 2062,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001174089.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000642402.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2110G>A",
          "hgvs_p": "p.Ala704Thr",
          "transcript": "ENST00000380056.7",
          "protein_id": "ENSP00000369396.3",
          "transcript_support_level": 1,
          "aa_start": 704,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 2110,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380056.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2191G>A",
          "hgvs_p": "p.Ala731Thr",
          "transcript": "NM_001174090.2",
          "protein_id": "NP_001167561.1",
          "transcript_support_level": null,
          "aa_start": 731,
          "aa_end": null,
          "aa_length": 918,
          "cds_start": 2191,
          "cds_end": null,
          "cds_length": 2757,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001174090.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2191G>A",
          "hgvs_p": "p.Ala731Thr",
          "transcript": "ENST00000380059.7",
          "protein_id": "ENSP00000369399.3",
          "transcript_support_level": 2,
          "aa_start": 731,
          "aa_end": null,
          "aa_length": 918,
          "cds_start": 2191,
          "cds_end": null,
          "cds_length": 2757,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000380059.7"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2110G>A",
          "hgvs_p": "p.Ala704Thr",
          "transcript": "NM_032034.4",
          "protein_id": "NP_114423.1",
          "transcript_support_level": null,
          "aa_start": 704,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 2110,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_032034.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2077G>A",
          "hgvs_p": "p.Ala693Thr",
          "transcript": "NM_001400280.1",
          "protein_id": "NP_001387209.1",
          "transcript_support_level": null,
          "aa_start": 693,
          "aa_end": null,
          "aa_length": 880,
          "cds_start": 2077,
          "cds_end": null,
          "cds_length": 2643,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400280.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2056G>A",
          "hgvs_p": "p.Ala686Thr",
          "transcript": "ENST00000925552.1",
          "protein_id": "ENSP00000595611.1",
          "transcript_support_level": null,
          "aa_start": 686,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 2056,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925552.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2005G>A",
          "hgvs_p": "p.Ala669Thr",
          "transcript": "NM_001400277.1",
          "protein_id": "NP_001387206.1",
          "transcript_support_level": null,
          "aa_start": 669,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 2005,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400277.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2005G>A",
          "hgvs_p": "p.Ala669Thr",
          "transcript": "NM_001400278.1",
          "protein_id": "NP_001387207.1",
          "transcript_support_level": null,
          "aa_start": 669,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 2005,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400278.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2005G>A",
          "hgvs_p": "p.Ala669Thr",
          "transcript": "NM_001400279.1",
          "protein_id": "NP_001387208.1",
          "transcript_support_level": null,
          "aa_start": 669,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 2005,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001400279.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1993G>A",
          "hgvs_p": "p.Ala665Thr",
          "transcript": "ENST00000644011.1",
          "protein_id": "ENSP00000496214.1",
          "transcript_support_level": null,
          "aa_start": 665,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 1993,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000644011.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.2062G>A",
          "hgvs_p": "p.Ala688Thr",
          "transcript": "ENST00000876657.1",
          "protein_id": "ENSP00000546716.1",
          "transcript_support_level": null,
          "aa_start": 688,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 2062,
          "cds_end": null,
          "cds_length": 2556,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876657.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1990G>A",
          "hgvs_p": "p.Ala664Thr",
          "transcript": "ENST00000925551.1",
          "protein_id": "ENSP00000595610.1",
          "transcript_support_level": null,
          "aa_start": 664,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 1990,
          "cds_end": null,
          "cds_length": 2556,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925551.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1987G>A",
          "hgvs_p": "p.Ala663Thr",
          "transcript": "ENST00000876658.1",
          "protein_id": "ENSP00000546717.1",
          "transcript_support_level": null,
          "aa_start": 663,
          "aa_end": null,
          "aa_length": 850,
          "cds_start": 1987,
          "cds_end": null,
          "cds_length": 2553,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876658.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1948G>A",
          "hgvs_p": "p.Ala650Thr",
          "transcript": "NM_001363745.2",
          "protein_id": "NP_001350674.1",
          "transcript_support_level": null,
          "aa_start": 650,
          "aa_end": null,
          "aa_length": 837,
          "cds_start": 1948,
          "cds_end": null,
          "cds_length": 2514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363745.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1948G>A",
          "hgvs_p": "p.Ala650Thr",
          "transcript": "ENST00000647296.1",
          "protein_id": "ENSP00000495050.1",
          "transcript_support_level": null,
          "aa_start": 650,
          "aa_end": null,
          "aa_length": 837,
          "cds_start": 1948,
          "cds_end": null,
          "cds_length": 2514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000647296.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1942G>A",
          "hgvs_p": "p.Ala648Thr",
          "transcript": "ENST00000876659.1",
          "protein_id": "ENSP00000546718.1",
          "transcript_support_level": null,
          "aa_start": 648,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 1942,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000876659.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1918G>A",
          "hgvs_p": "p.Ala640Thr",
          "transcript": "ENST00000925550.1",
          "protein_id": "ENSP00000595609.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 827,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 2484,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925550.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1933G>A",
          "hgvs_p": "p.Ala645Thr",
          "transcript": "ENST00000644692.1",
          "protein_id": "ENSP00000493824.1",
          "transcript_support_level": null,
          "aa_start": 645,
          "aa_end": null,
          "aa_length": 808,
          "cds_start": 1933,
          "cds_end": null,
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      "dbsnp": "rs748362724",
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      "gnomad_exomes_af": 0.00000273687,
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      "computational_score_selected": 0.9094411134719849,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.11999999731779099,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.793,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.5444,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.08,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.841,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.12,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
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            "PP5"
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          "verdict": "Uncertain_significance",
          "transcript": "NM_001174090.2",
          "gene_symbol": "SLC4A11",
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          "hgvs_p": "p.Ala731Thr"
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      ],
      "clinvar_disease": "Corneal dystrophy-perceptive deafness syndrome,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:1 US:1",
      "phenotype_combined": "Corneal dystrophy-perceptive deafness syndrome|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
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  ],
  "message": null
}