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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-3229430-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=3229430&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 3229430,
      "ref": "G",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "ENST00000642402.1",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1765C>T",
          "hgvs_p": "p.Arg589*",
          "transcript": "NM_001174089.2",
          "protein_id": "NP_001167560.1",
          "transcript_support_level": null,
          "aa_start": 589,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 1765,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": 1818,
          "cdna_end": null,
          "cdna_length": 3046,
          "mane_select": "ENST00000642402.1",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1765C>T",
          "hgvs_p": "p.Arg589*",
          "transcript": "ENST00000642402.1",
          "protein_id": "ENSP00000493503.1",
          "transcript_support_level": null,
          "aa_start": 589,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 1765,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": 1818,
          "cdna_end": null,
          "cdna_length": 3046,
          "mane_select": "NM_001174089.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1813C>T",
          "hgvs_p": "p.Arg605*",
          "transcript": "ENST00000380056.7",
          "protein_id": "ENSP00000369396.3",
          "transcript_support_level": 1,
          "aa_start": 605,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 1813,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": 1861,
          "cdna_end": null,
          "cdna_length": 3094,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1894C>T",
          "hgvs_p": "p.Arg632*",
          "transcript": "NM_001174090.2",
          "protein_id": "NP_001167561.1",
          "transcript_support_level": null,
          "aa_start": 632,
          "aa_end": null,
          "aa_length": 918,
          "cds_start": 1894,
          "cds_end": null,
          "cds_length": 2757,
          "cdna_start": 2017,
          "cdna_end": null,
          "cdna_length": 3245,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1894C>T",
          "hgvs_p": "p.Arg632*",
          "transcript": "ENST00000380059.7",
          "protein_id": "ENSP00000369399.3",
          "transcript_support_level": 2,
          "aa_start": 632,
          "aa_end": null,
          "aa_length": 918,
          "cds_start": 1894,
          "cds_end": null,
          "cds_length": 2757,
          "cdna_start": 1996,
          "cdna_end": null,
          "cdna_length": 3229,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1813C>T",
          "hgvs_p": "p.Arg605*",
          "transcript": "NM_032034.4",
          "protein_id": "NP_114423.1",
          "transcript_support_level": null,
          "aa_start": 605,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 1813,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": 1861,
          "cdna_end": null,
          "cdna_length": 3089,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1780C>T",
          "hgvs_p": "p.Arg594*",
          "transcript": "NM_001400280.1",
          "protein_id": "NP_001387209.1",
          "transcript_support_level": null,
          "aa_start": 594,
          "aa_end": null,
          "aa_length": 880,
          "cds_start": 1780,
          "cds_end": null,
          "cds_length": 2643,
          "cdna_start": 1905,
          "cdna_end": null,
          "cdna_length": 3133,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1708C>T",
          "hgvs_p": "p.Arg570*",
          "transcript": "NM_001400277.1",
          "protein_id": "NP_001387206.1",
          "transcript_support_level": null,
          "aa_start": 570,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 1708,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": 1934,
          "cdna_end": null,
          "cdna_length": 3162,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1708C>T",
          "hgvs_p": "p.Arg570*",
          "transcript": "NM_001400278.1",
          "protein_id": "NP_001387207.1",
          "transcript_support_level": null,
          "aa_start": 570,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 1708,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": 1933,
          "cdna_end": null,
          "cdna_length": 3161,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1708C>T",
          "hgvs_p": "p.Arg570*",
          "transcript": "NM_001400279.1",
          "protein_id": "NP_001387208.1",
          "transcript_support_level": null,
          "aa_start": 570,
          "aa_end": null,
          "aa_length": 856,
          "cds_start": 1708,
          "cds_end": null,
          "cds_length": 2571,
          "cdna_start": 1924,
          "cdna_end": null,
          "cdna_length": 3152,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1696C>T",
          "hgvs_p": "p.Arg566*",
          "transcript": "ENST00000644011.1",
          "protein_id": "ENSP00000496214.1",
          "transcript_support_level": null,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 852,
          "cds_start": 1696,
          "cds_end": null,
          "cds_length": 2559,
          "cdna_start": 1749,
          "cdna_end": null,
          "cdna_length": 2961,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Arg551*",
          "transcript": "NM_001363745.2",
          "protein_id": "NP_001350674.1",
          "transcript_support_level": null,
          "aa_start": 551,
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          "aa_length": 837,
          "cds_start": 1651,
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          "cds_length": 2514,
          "cdna_start": 1704,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Arg551*",
          "transcript": "ENST00000647296.1",
          "protein_id": "ENSP00000495050.1",
          "transcript_support_level": null,
          "aa_start": 551,
          "aa_end": null,
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          "cds_start": 1651,
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          "cdna_start": 1704,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1636C>T",
          "hgvs_p": "p.Arg546*",
          "transcript": "ENST00000644692.1",
          "protein_id": "ENSP00000493824.1",
          "transcript_support_level": null,
          "aa_start": 546,
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          "aa_length": 808,
          "cds_start": 1636,
          "cds_end": null,
          "cds_length": 2427,
          "cdna_start": 1852,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1813C>T",
          "hgvs_p": "p.Arg605*",
          "transcript": "XM_047440540.1",
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1708C>T",
          "hgvs_p": "p.Arg570*",
          "transcript": "XM_017028094.2",
          "protein_id": "XP_016883583.1",
          "transcript_support_level": null,
          "aa_start": 570,
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          "aa_length": 856,
          "cds_start": 1708,
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          "cdna_start": 1741,
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          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1708C>T",
          "hgvs_p": "p.Arg570*",
          "transcript": "XM_017028096.2",
          "protein_id": "XP_016883585.1",
          "transcript_support_level": null,
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          "aa_length": 856,
          "cds_start": 1708,
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          "cdna_start": 1969,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
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          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1594C>T",
          "hgvs_p": "p.Arg532*",
          "transcript": "XM_047440543.1",
          "protein_id": "XP_047296499.1",
          "transcript_support_level": null,
          "aa_start": 532,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1894C>T",
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          "transcript": "XM_047440541.1",
          "protein_id": "XP_047296497.1",
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          "cds_start": 1894,
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          "cdna_start": 2019,
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          "cdna_length": 2489,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SLC4A11",
          "gene_hgnc_id": 16438,
          "hgvs_c": "c.1824C>T",
          "hgvs_p": "p.Cys608Cys",
          "transcript": "XM_017028093.2",
          "protein_id": "XP_016883582.2",
          "transcript_support_level": null,
          "aa_start": 608,
          "aa_end": null,
          "aa_length": 878,
          "cds_start": 1824,
          "cds_end": null,
          "cds_length": 2637,
          "cdna_start": 1949,
          "cdna_end": null,
          "cdna_length": 3132,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
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        {
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      ],
      "gene_symbol": "SLC4A11",
      "gene_hgnc_id": 16438,
      "dbsnp": "rs121909390",
      "frequency_reference_population": 0.000009298658,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 15,
      "gnomad_exomes_af": 0.00000889845,
      "gnomad_genomes_af": 0.0000131399,
      "gnomad_exomes_ac": 13,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6200000047683716,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.62,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 4.866,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 16,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 16,
          "benign_score": 0,
          "pathogenic_score": 16,
          "criteria": [
            "PVS1",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000642402.1",
          "gene_symbol": "SLC4A11",
          "hgnc_id": 16438,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1765C>T",
          "hgvs_p": "p.Arg589*"
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      ],
      "clinvar_disease": " 4, Fuchs endothelial,Congenital hereditary endothelial dystrophy of cornea,Corneal dystrophy,Corneal dystrophy-perceptive deafness syndrome,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:2",
      "phenotype_combined": "Congenital hereditary endothelial dystrophy of cornea|not provided|Corneal dystrophy-perceptive deafness syndrome|Corneal dystrophy-perceptive deafness syndrome;Corneal dystrophy, Fuchs endothelial, 4;Congenital hereditary endothelial dystrophy of cornea",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}