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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-33360391-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=33360391&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 33360391,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001365728.1",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "NM_016408.4",
          "protein_id": "NP_057492.2",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000346416.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016408.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "ENST00000346416.7",
          "protein_id": "ENSP00000217372.2",
          "transcript_support_level": 1,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_016408.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000346416.7"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1412T>C",
          "hgvs_p": "p.Leu471Pro",
          "transcript": "ENST00000339269.5",
          "protein_id": "ENSP00000341840.5",
          "transcript_support_level": 1,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 510,
          "cds_start": 1412,
          "cds_end": null,
          "cds_length": 1533,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000339269.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1736T>C",
          "hgvs_p": "p.Leu579Pro",
          "transcript": "ENST00000874266.1",
          "protein_id": "ENSP00000544325.1",
          "transcript_support_level": null,
          "aa_start": 579,
          "aa_end": null,
          "aa_length": 618,
          "cds_start": 1736,
          "cds_end": null,
          "cds_length": 1857,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874266.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1694T>C",
          "hgvs_p": "p.Leu565Pro",
          "transcript": "ENST00000874267.1",
          "protein_id": "ENSP00000544326.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874267.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1685T>C",
          "hgvs_p": "p.Leu562Pro",
          "transcript": "NM_001365728.1",
          "protein_id": "NP_001352657.1",
          "transcript_support_level": null,
          "aa_start": 562,
          "aa_end": null,
          "aa_length": 601,
          "cds_start": 1685,
          "cds_end": null,
          "cds_length": 1806,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001365728.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1685T>C",
          "hgvs_p": "p.Leu562Pro",
          "transcript": "ENST00000357886.8",
          "protein_id": "ENSP00000350558.4",
          "transcript_support_level": 5,
          "aa_start": 562,
          "aa_end": null,
          "aa_length": 601,
          "cds_start": 1685,
          "cds_end": null,
          "cds_length": 1806,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000357886.8"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1670T>C",
          "hgvs_p": "p.Leu557Pro",
          "transcript": "ENST00000874261.1",
          "protein_id": "ENSP00000544320.1",
          "transcript_support_level": null,
          "aa_start": 557,
          "aa_end": null,
          "aa_length": 596,
          "cds_start": 1670,
          "cds_end": null,
          "cds_length": 1791,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874261.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1667T>C",
          "hgvs_p": "p.Leu556Pro",
          "transcript": "ENST00000912373.1",
          "protein_id": "ENSP00000582432.1",
          "transcript_support_level": null,
          "aa_start": 556,
          "aa_end": null,
          "aa_length": 595,
          "cds_start": 1667,
          "cds_end": null,
          "cds_length": 1788,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912373.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1646T>C",
          "hgvs_p": "p.Leu549Pro",
          "transcript": "NM_016082.4",
          "protein_id": "NP_057166.4",
          "transcript_support_level": null,
          "aa_start": 549,
          "aa_end": null,
          "aa_length": 588,
          "cds_start": 1646,
          "cds_end": null,
          "cds_length": 1767,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016082.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "ENST00000874262.1",
          "protein_id": "ENSP00000544321.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874262.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "ENST00000874268.1",
          "protein_id": "ENSP00000544327.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874268.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "ENST00000912367.1",
          "protein_id": "ENSP00000582426.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912367.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "ENST00000912374.1",
          "protein_id": "ENSP00000582433.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
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          "biotype": "protein_coding",
          "feature": "ENST00000912374.1"
        },
        {
          "aa_ref": "L",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "ENST00000945688.1",
          "protein_id": "ENSP00000615747.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
          "cds_end": null,
          "cds_length": 1764,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945688.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1643T>C",
          "hgvs_p": "p.Leu548Pro",
          "transcript": "ENST00000945690.1",
          "protein_id": "ENSP00000615749.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 587,
          "cds_start": 1643,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000945690.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1640T>C",
          "hgvs_p": "p.Leu547Pro",
          "transcript": "NM_001278167.2",
          "protein_id": "NP_001265096.1",
          "transcript_support_level": null,
          "aa_start": 547,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 1640,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001278167.2"
        },
        {
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1640T>C",
          "hgvs_p": "p.Leu547Pro",
          "transcript": "ENST00000874263.1",
          "protein_id": "ENSP00000544322.1",
          "transcript_support_level": null,
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          "aa_length": 586,
          "cds_start": 1640,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "L",
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          "protein_coding": true,
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          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1562T>C",
          "hgvs_p": "p.Leu521Pro",
          "transcript": "NM_001278169.1",
          "protein_id": "NP_001265098.1",
          "transcript_support_level": null,
          "aa_start": 521,
          "aa_end": null,
          "aa_length": 560,
          "cds_start": 1562,
          "cds_end": null,
          "cds_length": 1683,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001278169.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CDK5RAP1",
          "gene_hgnc_id": 15880,
          "hgvs_c": "c.1535T>C",
          "hgvs_p": "p.Leu512Pro",
          "transcript": "ENST00000945691.1",
          "protein_id": "ENSP00000615750.1",
          "transcript_support_level": null,
          "aa_start": 512,
          "aa_end": null,
          "aa_length": 551,
          "cds_start": 1535,
          "cds_end": null,
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          "biotype": "pseudogene",
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        {
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          "protein_coding": false,
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          "consequences": [
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          "exon_rank": 7,
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          "gene_symbol": "CDK5RAP1",
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          "hgvs_c": "n.818T>C",
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          "transcript": "ENST00000496381.5",
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          "biotype": "pseudogene",
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        {
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            "intron_variant"
          ],
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          "gene_symbol": "CDK5RAP1",
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          "hgvs_c": "n.535-1304T>C",
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          "transcript": "ENST00000498525.5",
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000498525.5"
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      ],
      "gene_symbol": "CDK5RAP1",
      "gene_hgnc_id": 15880,
      "dbsnp": "rs970247691",
      "frequency_reference_population": 0.0000074352297,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 12,
      "gnomad_exomes_af": 0.00000752512,
      "gnomad_genomes_af": 0.00000657168,
      "gnomad_exomes_ac": 11,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.14071473479270935,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.131,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0874,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.414,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001365728.1",
          "gene_symbol": "CDK5RAP1",
          "hgnc_id": 15880,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1685T>C",
          "hgvs_p": "p.Leu562Pro"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}