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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-33659657-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=33659657&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 33659657,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_031232.4",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "NM_031232.4",
          "protein_id": "NP_112509.3",
          "transcript_support_level": null,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 396,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 1191,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000246190.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031232.4"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "ENST00000246190.11",
          "protein_id": "ENSP00000246190.6",
          "transcript_support_level": 5,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 396,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 1191,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_031232.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000246190.11"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "ENST00000375238.8",
          "protein_id": "ENSP00000364386.4",
          "transcript_support_level": 1,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375238.8"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "ENST00000883747.1",
          "protein_id": "ENSP00000553806.1",
          "transcript_support_level": null,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883747.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "NM_031231.4",
          "protein_id": "NP_112508.3",
          "transcript_support_level": null,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 362,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 1089,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031231.4"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.767G>A",
          "hgvs_p": "p.Cys256Tyr",
          "transcript": "ENST00000883746.1",
          "protein_id": "ENSP00000553805.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 360,
          "cds_start": 767,
          "cds_end": null,
          "cds_length": 1083,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883746.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "ENST00000883745.1",
          "protein_id": "ENSP00000553804.1",
          "transcript_support_level": null,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 352,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 1059,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883745.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "ENST00000606690.5",
          "protein_id": "ENSP00000475420.1",
          "transcript_support_level": 5,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000606690.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "ENST00000439478.5",
          "protein_id": "ENSP00000392064.1",
          "transcript_support_level": 5,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 288,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 868,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000439478.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.56G>A",
          "hgvs_p": "p.Cys19Tyr",
          "transcript": "ENST00000480994.5",
          "protein_id": "ENSP00000475834.1",
          "transcript_support_level": 5,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 124,
          "cds_start": 56,
          "cds_end": null,
          "cds_length": 377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000480994.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr",
          "transcript": "XM_005260510.1",
          "protein_id": "XP_005260567.1",
          "transcript_support_level": null,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 344,
          "cds_start": 719,
          "cds_end": null,
          "cds_length": 1035,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005260510.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.350G>A",
          "hgvs_p": "p.Cys117Tyr",
          "transcript": "XM_047440369.1",
          "protein_id": "XP_047296325.1",
          "transcript_support_level": null,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 350,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440369.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.350G>A",
          "hgvs_p": "p.Cys117Tyr",
          "transcript": "XM_047440370.1",
          "protein_id": "XP_047296326.1",
          "transcript_support_level": null,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 239,
          "cds_start": 350,
          "cds_end": null,
          "cds_length": 720,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047440370.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.236G>A",
          "hgvs_p": "p.Cys79Tyr",
          "transcript": "XM_011528991.2",
          "protein_id": "XP_011527293.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 201,
          "cds_start": 236,
          "cds_end": null,
          "cds_length": 606,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011528991.2"
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "c.236G>A",
          "hgvs_p": "p.Cys79Tyr",
          "transcript": "XM_017028016.2",
          "protein_id": "XP_016883505.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 201,
          "cds_start": 236,
          "cds_end": null,
          "cds_length": 606,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017028016.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "n.482G>A",
          "hgvs_p": null,
          "transcript": "ENST00000463246.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000463246.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "n.358G>A",
          "hgvs_p": null,
          "transcript": "ENST00000478237.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000478237.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "n.540G>A",
          "hgvs_p": null,
          "transcript": "ENST00000483813.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000483813.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "n.*195G>A",
          "hgvs_p": null,
          "transcript": "ENST00000485399.5",
          "protein_id": "ENSP00000475369.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000485399.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NECAB3",
          "gene_hgnc_id": 15851,
          "hgvs_c": "n.548G>A",
          "hgvs_p": null,
          "transcript": "ENST00000485976.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000485976.5"
        },
        {
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        {
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          "protein_coding": false,
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          "consequences": [
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          "biotype": "pseudogene",
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        {
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          "consequences": [
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          "gene_symbol": "NECAB3",
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          "transcript": "ENST00000493590.1",
          "protein_id": "ENSP00000475755.1",
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          "biotype": "nonsense_mediated_decay",
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        },
        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "NECAB3",
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          "hgvs_c": "n.*2G>A",
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          "transcript": "ENST00000498353.5",
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          "transcript_support_level": 3,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000498353.5"
        }
      ],
      "gene_symbol": "NECAB3",
      "gene_hgnc_id": 15851,
      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.32144778966903687,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.085,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1135,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.33,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_031232.4",
          "gene_symbol": "NECAB3",
          "hgnc_id": 15851,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.719G>A",
          "hgvs_p": "p.Cys240Tyr"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}